Thyroidal hormones restore cell proliferation to the lenses of hypophysectomized adult frogs.
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Biomedical subjects
Publications and source records attributed to L Roberts.
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Occasionally in cases of porcine intestinal adenomatosis (PIA) epithelial dysplasia is seen with infiltration of the epithelium into underlying tissues and spread, via the lymphatics, to drainage lymph nodes. The intracellular bacterium Campylobacter sputorum subspecies mucosalis, associated with PIA, can be demonstrated in the epithelial cells of the metastases. This dysplasia and infiltration appears to be related to surface damage and inflammation.
Pigs dosed orally with Campylobacter sputorum subsp mucosalis in the neonatal period became infected and mucosalis was recovered from the intestinal mucosa for up to 40 days after infection. Rapid spread to undosed litter mates occurred. Gross adenomatous change did not develop.
The weaned pig proved resistant to infection with Campylobacter sputorum subsp mucosalis after oral dosing. Pretreatment with benzetimide to reduce gastrointestinal peristalsis allowed mucosalis infection to be established.
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Since January 1978 four outbreaks of bovine venereal campylobacteriosis, associated with Campylobacter fetus subspp fetus, have been investigated. The herds demonstrated a variety of presenting signs, and the outbreaks were confirmed by vaginal mucus agglutination tests and isolation of C fetus subspp fetus. A greater recovery of C fetus was achieved by reducing the oxygen tension in the atmosphere used for incubation to a greater extent than usual. Bradford's brucella agar was found to be a suitable selective media for C fetus.
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The weight gains of pigs in a hysterectomy-derived herd were evaluated using the cumulative sum technique. Representative animals growing poorly were selected by this method and a high proportion were found to be affected with porcine intestinal adenomatosis. Some possible effects of the condition on production in the herd were evaluated from these findings.
This study indicates that viable Campylobacter sputorum subsp mucosalis are not present or are present in small numbers in the mucosa of pigs dying of proliferative haemorrhagic enteropathy. The changes present in the mucosa are similar to those seen in pigs recovering from adenomatosis and the evidence obtained indicates that the intracellular organisms observed in this condition are indeed mucosalis. The presence of large amounts of IgA in the altered tissue of both proliferative haemorrhagic enteropathy and porcine intestinal adenomatosis indicates that the failure to recover bacteria may be immunologically mediated but is not simply related to the presence or absence of antibody in the respective conditions.
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Histochemical studies of normal and adenomatous intestinal mucosa of the pig showed the adenomatous epithelium to be deficient in enzymes normally found in mature absorptive cells. Electron microscopic studies confirmed that the adenomatous cells closely remsembled the crypt cells of normal mucosa.
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