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Biomedical subjects

L Reynolds

Publications and source records attributed to L Reynolds.

12 recordsLinked to original sources
PubMed1992

A polymerase chain reaction-based method for isolation of gene-specific sequences from the interferon-alpha gene cluster.

The interferon-alpha gene is a gene family of over 20 distinct genes having 80-95% homology with one another at a nucleotide level. Because of the high homology in the gene cluster, the available interferon-alpha gene probes can hybridize to multiple bands of different size on Southern blot analysis of restricted human genomic DNA. We used the polymerase chain reaction with the primers synthesized from Alu repetitive sequence and the conserved sequences of the interferon-alpha gene cluster to generate specific probes for individual interferon-alpha genes. The amplification products were subcloned into a plasmid vector and analyzed by DNA sequencing and Southern blotting of the restricted human placental DNA. One clone, which derived from interferon-alpha 14 gene, produced a single 5.2-kb band in Southern blots of the HindIII-restricted human placental DNA. This stands in contrast to the 10 bands of different size that were detected with a cDNA for the interferon-alpha I' gene. Our results indicate that a polymerase chain reaction-based method can be used to isolate gene-specific sequences from the interferon-alpha gene cluster. Since a variety of human cancers has been found to have the complete or partial deletion of the interferon-alpha gene cluster, the gene-specific probe generated by this method may aid in determining the breakpoints in the vicinity of the gene cluster.

Animals↗
PubMed1992

Cattle production systems in the derived Savannah and southern Guinea Savannah regions of Oyo State, southern Nigeria.

A single visit questionnaire survey was undertaken on 50 cattle owners in Oyo State, south-western Nigeria. Data were collected on grazing patterns, calving dates, calf mortalities and attitudes to the N'Dama breed and its crosses with Bunaji cattle. All cattle herds had similar grazing patterns, using natural pasture from April to November, crop residues in December, pasture regrowth after burning plus crop residues in January and regrowth and browse in February and March. The conception pattern was unimodal, reaching a peak in July after the new grass growth from the first rains. Calf mortalities were highest between 2 to 4 months of age during the early rainy season, but were significantly lower in mixed N'Dama/Bunaji herds than in the Bunaji herds. Most cattle owners were interested in purchasing N'Dama breeding stock, believing them to be more productive. This is a move away from the traditional pastoralist practice of keeping only Bos indicus breeds.

Animal Husbandry↗
PubMed1992

Hearing thresholds in CAPD patients.

Ultra high frequency (UHF, 10-20 kHz) and conventional audiometric thresholds (0,25-8 kHz) were obtained from 42 stable CAPD patients. Twenty-one (50%) and 11 (25%) of the patients had UHF and conventional hearing loss respectively, when compared to age related control data. This was unrelated to length of chronic renal failure and number of treatments with ototoxic drugs. Sixteen of these patients were monitored audiometrically using UHF during a course of vancomycin therapy for peritonitis. There was no significant change in hearing thresholds. In conclusion there is high incidence of hearing loss in CAPD patients which is unrelated to ototoxic drugs.

Adult↗
PubMed1991

High frequency thresholds: variations with age and industrial noise exposure.

The effects of age and noise exposure were investigated. 97 normal subjects representing 6 age groups were tested with both conventional pure tone audiometry and high frequency audiometry (10-20 kHz) using a commercial high frequency audiometer. Thresholds were obtained by means of earphones as opposed to a quasi-free field technique. The results were discussed in terms of the age effect. In addition, conventional and high frequency thresholds were obtained for 64 subjects who had a history of industrial noise exposure representing 4 age groups. Significant differences were found between the noise exposed group and the normal subjects. The implications of the results are discussed.

Adolescent↗
PubMed1979

Effect of micropore filtration on pulmonary function after massive transfusion.

1. This study demonstrates a positive correlation between the number and size of infused microaggregates and the subsequent abnormality in pulmonary function as measured by oxygenation and dead space. 2. No such correlation between the severity of injury and the altered pulmonary function or transfusion volume was demonstrated. 3. We were unable to demonstrate an advantage to the use of a 40 mu micropore filter in preventing the adult respiratory distress syndrome (ARDS) or in improving pulmonary function in our patients. 4. One explanation for the failure to demonstrate such an advantage is the low efficiency of the filter used.

Adolescent↗
PubMed1977

Intravenous infusion of glucose and insulin in relation to milk secretion in the sow.

1. A comparison was made of the composition of milk from front and rear tetas in four sows. There were small and not significant differences in fat, protein and lactose contents, and in the fatty-acid composition of the milk fat with the exception of the 18:3 acid where the difference was also small but significant. 2. The effects of intravenous infusions of glucose and insulin in lactating sows on milk secretion and blood composition were investigated in two sows. 3. Intravenous infusion of glucose had no effect on blood plasma glucose concentration but increased the yields of lactose, protein and water. 4. Intravenous infusion of insulin depressed plasma glucose concentration and the yields of lactose and water. The yield of protein was unaffected. 5. It is concluded that differences between the non-ruminant (the sow) and the ruminant in the responses in milk secretion to glucose infusion may be related to differences in the sensitivity to insulin of mammary tissue.

Animals↗
PubMed1976

Adding a Medex to the medical mix: an evaluation.

Three classes of Medex were followed during their preceptorship training and subsequent employment to assess the impact of adding such physician assistants on 13 private practices. The data suggest that the Medex are readily incorporated into the practice, seeing a similar spectrum of patients and generally functioning as a semicolleague. Although the Medex was almost universally a contributing addition to the practice, increasing patient volume, the specific effects varied with the setting.

Adolescent↗
PubMed1975

The profession.

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Australia↗