Biomedical subjects

L Ren

Publications and source records attributed to L Ren.

96 records · Page 6Linked to original sources

PubMed1986

Establishment of mutant murine mammary carcinoma FM3A cell strains transformed with the herpes simplex virus type 2 thymidine kinase gene.

To establish cell systems appropriate for investigating the mode of action of antiherpetic nucleoside analogues, mutant cell strains were constructed from murine mammary carcinoma FM3A cells, which were deficient in TK, but were transformed with a recombinant plasmid DNA containing the HSV-2 TK gene. The transformed cells incorporated the viral DNA, expressed viral TK activity and showed unusually high sensitivity to the cytostatic action of the antiherpetic nucleoside analogues ACV and IVDU, both of which were only weakly inhibitory to the growth of the parent cells. Curiously, the FM3A cell strains transformed with HSV-2 TK gene showed a higher sensitivity to ACV and IVDU than the previously established cell line transformed with HSV-1 TK gene. This contrasts with the inhibitory effects of ACV and IVDU on acute HSV infection, since HSV-2 infection is slightly or considerably less susceptible than HSV-1 infection to inhibition by ACV or IVDU, respectively.

Acyclovir↗

PubMed1984

UM-UC-1 and UM-UC-2: characterization of two new human transitional cell carcinoma lines.

Two new human transitional cell carcinoma lines have been established in long term tissue culture. UM-UC-1 was derived from a bladder cancer metastasis, and UM-UC-2 originated from a ureteral carcinoma. Both cell lines produce tumors in athymic nude mice. The cell lines have been characterized by isoenzyme phenotype and karyotype. Both of these methods differentiate UM-UC-1 from UM-UC-2.

Adult↗

PubMed1984

UM-SCP-1, a new human cell line derived from a prostatic squamous cell carcinoma.

A permanent cell line (UM-SCP-1) has been established from a primary squamous cell carcinoma of the prostate. UM-SCP-1 has been passaged 40 times and has been in culture for 22 months. The doubling time of this aneuploid cell line is approximately 36 hr. In nude mice, UM-SCP-1 produces rapidly growing tumors with distinct histological features of squamous cell carcinoma. UM-SCP-1 cells express pemphigus and pemphigoid antigens and bind antibodies to beta 2 microglobulin and HLA-A,B,C common antigen. Cells of this line are unreactive with anti-A and anti-B blood group typing sera, autologous serum, and monoclonal anti-HLA-DR antibodies.

Animals↗

PubMedDate not supplied

Septicemia and septic shock in pediatric patients: 140 consecutive cases on a pediatric hematology-oncology service.

PURPOSE: This report describes the incidence of septic shock in pediatric hematology-oncology patients with positive blood cultures and investigates parameters of potential use in early diagnosis of gram-negative (GN) bacteremia and septic shock. PATIENTS: In a 12-month period, 140 consecutive episodes of septicemia (135 bacterial and 5 fungal) were seen in 100 patients. The absolute neutrophil count (ANC) was > 500/microl in 89 episodes (65%). RESULTS: Septic shock developed in patients with positive blood cultures with an overall incidence of approximately 19%. Of the 12 bacteremic patients who required transfer to the intensive care unit, 83% had a GN isolate recovered. The incidence of septic shock was not significantly lower in the group of patients with ANC > 500/microl. Low serum bicarbonate correlated with GN infection in patients with bacteremia. CONCLUSIONS: GN organisms were the major cause of septic shock in a group of pediatric hematology-oncology patients with positive blood cultures although they were recovered less frequently than gram-positive organisms. In our study, non-neutropenic patients with indwelling catheters were at approximately the same risk for GN shock as neutropenic patients. Monitoring blood carbon dioxide content may be useful in the early diagnosis of GN infection.

Adolescent↗

PubMedDate not supplied

[Congenital total ectropion of upper lid].

Two cases with congenital total ectropion of the upper eyelids are reported (1 bilateral and 1 unilateral). There were no associated ocular or general abnormalities nor any apparent causes. One child was born normally, the other by cesarean section. The disease is not caused by difficult labor through natural birth passages. The condition is usually cured by conservative therapy. If surgery is carried out, it should be performed as early as possible in order to rule out secondary infections, amblyopia, epidermization of the conjunctiva, etc.

Ectropion↗

PubMedDate not supplied

Secretion of a soluble T cell promoting factor by the human prostate adenocarcinoma cell line DU-145.

INTRODUCTION: A variety of cancers suppress host immune defenses by secretion of soluble factors. Conditioned media (CM) from numerous cancer cell lines possess the ability to suppress proliferation of activated T cells. The effects of CM from the prostate cancer cell line DU-145 on T cell activation was investigated. MATERIALS AND METHODS: Human PBMC, purified T cells and Jurkat T cells were treated with DU-145 CM. Proliferation, cell cycle, apoptosis, Fas expression/and cytokine secretion were assessed by thymidine incorporation, flow cytometry and ELISA. RESULTS: DU-145 CM increased proliferation of concanavalin-A (ConA) activated peripheral blood mononuclear cells (PBMC) increasing the percentage of cells in the S/G2 phase of cell cycle. Treatment of the Jurkat T cell line with DU-145 CM induced a potent proliferative response. ConA-induced proliferation of purified T cells from human PBMC and murine splenocytes was augmented in a dose-dependent manner by addition of DU- 145 CM. DU- 145 CM treatment of ConA-activated T cells induced an increase in interleukin-2 (IL-2) production. The soluble factor(s) responsible for promoting T cell proliferation was dependent on protein synthesis by the DU- 145 cells and possessed a molecular weight greater than 10 kDa. CONCLUSION: DU- 145 cells secrete a soluble factor(s) > 10 kDa, whose production is dependent on protein synthesis and which acts as a promoter of T cell activation.

Adenocarcinoma↗