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Biomedical subjects

L R Overby

Publications and source records attributed to L R Overby.

At least 55 records · Page 3Linked to original sources
PubMed1975

Comparison of confirmation methods for hepatitis-B antigen and the nature of false-positives detected by 125I-immunoglobulins.

Immunologic specificity associated with solid-phase direct radioimmunoassay (RIA) using guinea pig antibodies for HBsAg detection (Ausria-125TM) was examined further with physicochemical techniques. The RIA reactivities which could not be neutralized by wide-spectrum human anti-HBs serum appeared to be physically distinct from true HBsAg particles; hence, they were truly false-positive, resulting from immunologic cross-reactions. In order to ensure the full advantages of using this highly sensitive RIA test, a confirmatory test using specific human anti-HBs for neutralization is therefore required to distinguish the true- and false-positives. The basic RIA technique is a two-step procedure. Two basic confirmation procedures, namely, a first-step neutralization and a second-step neutralization, were investigated in depth to assess their efficacy and practicality for confirmation. Both procedures were effective for confirmation purposes; however, the first-step neutralization procedure failed to confirm some high-titered HBsAg samples unless these samples were appropriately diluted. The second-step neutralization method did not require dilutions of any test samples.

Animals↗
PubMed1975

Antigenic subgroups of hepatitis B (surface) antigen in Berlin (West) and their epidemiological aspects.

Sera of 334 hepatitis B patients and 118 sera of HBsAg carriers were tested for the distribution of the subgroups ad and ay by means of rheophoresis technique or solid phase radiommunoassay. The distribution of subgroups revealed a typical pattern. The ad-antigen determinant was more frequent in hepatitis B patients, blood donors and hemodialysis patients whereas the ay determinant was detected in drug users, guest workers and juveniles at a considerably higher percentage. The difference in distribution is discussed as an epidemiological phenomenon of hepatitis B virus, dependent on environmental factors characteristic for certain groups of the population. The routine testing for HGsAg subgroups is recommended as a valuable epidemiologic tool.

Adolescent↗
PubMed1974

Inhibition of herpes simplex virus replication by phosphonoacetic acid.

Replication of herpes simplex virus in WI-38 cells was inhibited by phosphonoacetic acid, as measured by decreased virus cytopathogenic effect and incorporation of radiolabeled thymidine in virus-infected cells. The drug appeared to have no effect on adsorption, penetration, or release of the virus nor on the synthesis of ribonucleic acid or protein. It appeared to inhibit virus deoxyribonucleic acid synthesis.

Antiviral Agents↗
PubMed1973

Hepatitis B virus antigen: validation and immunologic characterization of low-titer serums with 125 I-antibody.

Radioimmunnoassay was used to determine the serologic subspecificities of 85 blood donor serums positive for hepatitis B virus-associated antigen. There was complete agreement with results obtained by immunoprecipitatiotn of 43 serums. The remaining 42 serums were negative by immunoprecipitation but followed type-specific immunology by radioimmunoassay, and this served as a validation for authentic hepatitis B virus antigen.

Animals↗
PubMed1973

Suppression of herpes simplex virus infection by phosphonoacetic acid.

Disodium phosphonoacetate when administered orally or topically to mice experimentally infected with herpes simplex virus was able to significantly reduce the mortality associated with the agent. In addition, this compound was able to reduce herpesvirus lesions on the corneas of infected rabbits.

Acetates↗
PubMed1969

Self-assembly of Q-beta and MS2 phage particles: possible function of initiation complexes.

Four kinds of particles were reconstituted with RNA and protein from the genetically unrelated bacteriophages Qbeta and MS2, namely, two homologous and two heterologous, with respect to RNA and protein. However, once Qbeta RNA (or MS2 RNA) reacted with a few molecules of either Qbeta or MS2 protein to form a nucleoprotein complex (initiation complex), it formed a phagelike particle only with subsequent addition of the same protein.

Centrifugation, Density Gradient↗