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Biomedical subjects

L Plouffe

Publications and source records attributed to L Plouffe.

49 records · Page 3Linked to original sources
PubMed1992

Gonadotropin-releasing hormone, follicle-stimulating hormone beta, luteinizing hormone beta gene structure in idiopathic hypogonadotropic hypogonadism.

OBJECTIVE: To determine if the genes for gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone beta (FSH beta), and luteinizing hormone beta (LH beta) are present, and if so, whether gene structure is normal in patients with idiopathic hypogonadotropic hypogonadism (IHH). DESIGN: Patients with clinical and laboratory characteristics of IHH were studied at the deoxyribonucleic acid (DNA) level to assess gene structure. SETTING: This study took place in an academic setting. PATIENTS: Human volunteers with documented IHH and fertile controls were studied. INTERVENTIONS: Genomic DNAs were extracted from each patient, Southern blots were constructed and hybridized to DNA probes for GnRH, FSH beta, and LH beta. DNA samples were also subjected to polymerase chain reaction analysis. MAIN OUTCOME MEASURES: Gene structure was assessed by analysis of autoradiographs and gel electrophoresis of polymerase chain reaction products in both the study patients and controls. RESULTS: Each analysis for FSH beta, LH beta, and GnRH demonstrated the same sized fragments in both the study group and control group. A 1.2-kilobase fragment containing the coding region for GnRH was present in all patients with IHH and controls by polymerase chain reaction. CONCLUSIONS: The genes for GnRH, LH beta, and FSH beta are present in patients with IHH. No large deletions or rearrangements of any of these genes were identified in any of these patients.

Base Sequence↗
PubMed1990

5-fluorouracil exposure during the period of conception: report on two cases.

Two patients who were administered topical vaginal 5-fluorouracil for human papilloma virus lesions became pregnant during treatment. The outcomes in both cases were healthy with normal developmental landmarks at 6 months of age. This experience highlights the potential for a positive outcome.

Administration, Intravaginal↗
PubMed1990

The detection of Hind III restriction-fragment length polymorphisms using a deoxyribonucleic acid probe for the beta subunit of follicle-stimulating hormone.

Molecular diagnosis of disorders of follicle-stimulating hormone (FSH) production may become possible now that the gene for FSH beta has been characterized. Restriction-fragment length polymorphism (RFLP) analysis provides a means of organized search for molecular variants of FSH. The purpose of this study was to screen controls for the presence of RFLPs using the deoxyribonucleic acid (DNA) probe pFSH beta -1.4. Genomic DNA was digested with 12 different restriction endonucleases; Southern blots were constructed and hybridized to pFSH beta -1.4. No polymorphisms were identified with 11 enzymes. Three of 24 (12.6%) Hind III digests demonstrated a polymorphic fragment of either 5.2, 4.7, or 4.3 kb. These are the first RFLPs identified for the FSH beta gene with pFSH beta -1.4. RFLPs for FSH beta constitute the first step in the molecular analysis of disorders of FSH production.

Blotting, Southern↗
PubMed1990

Deoxyribonucleic acid analysis of the zinc finger Y gene in 45,X/46,XY subjects.

The deoxyribonucleic acid from nine subjects with a 45,X/46,XY karyotype with a cytogenetically intact Y chromosome and phenotypically presenting with bilateral streak gonads, streak and testis, or bilateral scrotal testes along with a control male and female were analyzed for the presence of the zinc finger Y sequence through the molecular probe pDP1007. This particular probe is thought to constitute part of the putative testicular-determining factor gene. All the study subjects demonstrated the presence of zinc finger Y. Laser densitometry studies confirmed a correlation between the intensity of the zinc finger Y band and the percentage of Y cell lines. This study supports the fact that individuals with mixed gonadal dysgenesis and cytogenetically intact Y chromosomes will tend to have intact zinc finger Y sequences.

Adolescent↗
PubMed1989

Age, sex, and personality correlates of self-actualization in elderly adults.

The present study examined age, sex and personality correlates of self-actualization among older adults. The Personal Orientation Inventory and Personality Research Form were administered individually to 80 community men and women aged 56 yr.-84 yr. No main effect for sex was observed on inventory scores, but younger subjects (56 yr.-67 yr.) obtained higher scores than older subjects (68 yr.-84 yr.) on four subscales: Existentiality, Feeling Reactivity, Acceptance of Aggression and Capacity for Intimate Contact. Age was negatively correlated with Feeling Reactivity in both sexes; Inner-directedness decreased with age in men and Capacity for Intimate Contact diminished with age in women. For men, inventory scores were positively associated with the traits of Change and Endurance and negatively correlated with Abasement, Order and Succorance. For women, Impulsivity correlated positively and Order was associated negatively with scores on the inventory. These results suggest that the components of self-actualization are differentially influenced by age for men and women and that the traits accompanying self-actualization are sex-linked.

Aged↗
PubMed1985

Screening for sexual problems through a simple questionnaire.

Screening for sexual problems should be part of the medical assessment, but there is controversy on the best method to use. This study compared the yield of a simple questionnaire with that of a more detailed interview. Ninety-eight sexually active women (out of a total of 110) admitted on an elective basis to general gynecology wards were studied. Forty-six patients presented with a sexual complaint and 33 (33%) of these requested medical attention for the problem. In all cases, the simple questionnaire was as effective as the detailed inquiry in detecting a sexual problem. It is concluded that a simple questionnaire can be used effectively by all physicians as a screening test for sexual dysfunction with regard to heterosexual coital activity. Further research is required to confirm this hypothesis in other conditions of medical practice.

Adult↗
PubMed1984

Non-Hodgkin's lymphoma in Bartholin's gland: case report and review of literature.

Described in this report is a systemic lymphoma which had selectively involved the Bartholin gland. The true prevalence of this phenomenon is not known, but greater attention to these areas of the genital tract may uncover additional cases and provide better insight into the natural history of lymphomas that involve the lower female genital tract.

Bartholin's Glands↗
PubMed1982

Effect of saliva on sperm motility and activity.

Infertile couples frequently experience sexual dysfunction during their infertility investigations, including inadequate vaginal lubrication. In a attempt to look for a lubricant that would not impair sperm motility and activity, saliva was added to normal semen from healthy male donors. Saliva induced a "shaking movement" in 12% of the total sperm population incubated with high concentrations of saliva. This phenomenon did not occur with low concentrations of saliva, but sperm motility and progression significantly decreased. The results indicate that saliva has a deleterious effect on sperm motility and activity and should not be encouraged as a vaginal lubricant for the infertile couple.

Adult↗
PubMed1979

Neuroticism and the effect of stress on the pupillary light reflex.

The pupillary light reflex was recorded under control, stress and fatigue conditions for 30 subjects equally divided into groups low, moderate, and high in Neuroticism. The effects of stress were distinguished by a decrease in both latency and extent of pupillary constriction during the initial light stimulation trials, as well as by a greater magnitude of redilation. Latency to maximum constriction and extent of constriction and redilation progressively decreased during the fatigue condition. No differences between Neuroticism groups were observed although it was noted that Neuroticism was negatively correlated with magnitude of redilation during the post-stress condition.

Humans↗
PubMedDate not supplied

Selective estrogen receptor modulators (SERMs) in clinical practice.

The objective of this literature review is to familiarize the reader with the clinical data on selective estrogen receptor modulators (SERMs) and antiestrogens currently in use in the US, excluding data on breast effects. Four compounds in the SERM and antiestrogen families are presently in clinical use in the US: clomiphene (CC), tamoxifen (TAM), toremifene (TOR), and raloxifene (RLX). The clinical database on these compounds is among the largest available. Each compound demonstrates a specific profile for its target tissue effects, and this may differ between premenopausal and postmenopausal women. CC is the most widely used agent for ovulation induction. TAM is indicated in the management of breast cancer and for prevention in women at high risk. TAM may have additional effects on the cardiovascular and skeletal systems. TOR also is used for its effects on breast tissue and may have positive cardiovascular effects. RLX is approved in the management of osteoporosis with data supporting favorable effects on the cardiovascular system and breast tissue. TAM and TOR appear to have stimulatory effects on the uterus and endometrium, whereas RLX is neutral. Few adverse events have been attributed to these agents, with hot flashes being the most common one. There appears to be an increased risk of thromboembolic events with continuous use of TAM, TOR, and RLX. SERMs and antiestrogens continue to be studied extensively. Their evolving profiles support key roles for these agents in modern day medicine, particularly in the management of postmenopausal women's health.

Breast Neoplasms↗
PubMedDate not supplied

A pharmacological review of selective oestrogen receptor modulators.

Selective oestrogen receptor modulators (SERMs) are structurally diverse non-steroidal compounds that bind to oestrogen receptors and produce oestrogen agonist effects in some tissues and oestrogen antagonist effects in others. SERMs are being evaluated for a number of oestrogen-related diseases, including post-menopausal osteoporosis, hormone-dependent cancers, and cardiovascular disease. Several compounds that exhibit a SERM profile are currently available for clinical use, including clomiphene, tamoxifen, and toremifene (which are triphenylethylenes) and raloxifene (a benzothiophene). Clomiphene is used for the induction of ovulation in sub-fertile women attempting pregnancy. Tamoxifen and toremifene are both used to treat breast cancer. Tamoxifen may have beneficial effects on bone mineral density and serum lipids. The effects of toremifene on serum lipids are similar to that of tamoxifen. Both compounds have stimulatory effects on the endometrium. Raloxifene, indicated for the treatment and prevention of post-menopausal osteoporosis, has beneficial effects on bone mineral density and serum lipids, but does not increase the risk of endometrial hyperplasia or endometrial cancer. Recently, raloxifene was shown to reduce the incidence of vertebral fractures in otherwise healthy women with osteoporosis; in the same study, a reduced incidence of breast cancer was also observed. Similar to oestrogens, SERMs increase the incidence of venous thromboembolism. Several newer compounds that exhibit a SERM profile are also in clinical development, including other triphenylethylenes (droloxifene, idoxifene) and benzothiophenes (LY353381.HCl), benzopyrans (EM-800), and naphthalenes (CP-336,156).

Breast Neoplasms↗