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Biomedical subjects

L Perelmutter

Publications and source records attributed to L Perelmutter.

12 recordsLinked to original sources
PubMed1979

Immunoglobulin E response during viral infections.

One hundred and three patients (90 nonatopics and 13 atopics) with respiratory infections to various viral agents were studied retrospectively with respect to IgE immunoglobulin levels during acute (1 to 7 days) and convalescent (8 to 30 days) phases of infection. It was found that 59% of patients had a decrease of 20% or more in IgE level, 27% remained the same, and only 14% showed a rise 20% or more from the acute to the convalescent phases of infection. IgE levels decreased up to 3 to 4 wk after symptoms and the degree of decrease was more apparent for the nonatopics who had higher IgE levels in their acute phase of infection. Less dramatic decrease in IgE was observed for the 13 atopics studied. The changes in IgE levels during the viral infectious period are discussed in terms of possible cellular mechanisms that may control IgE immunoglobulin.

Acute Disease↗
PubMed1978

Possible role of IgG4 in discordant correlations between intracutaneous skin tests and RAST.

Comparison of intracutaneous skin tests and RAST in 2 groups of patients, one consisting of 16 individuals having multiple allergies to pollen, mold and animal dander and the other of 10 patients allergic to mold only, revealed that skin tests were more sensitive than RAST. Skin reactions of 3+ to 4+ were frequently associated with negative RAST results. This was observed more so in mold-allergic patients. In a few cases (6%) RAST showed 2+ reactions while the skin tests were negative. Leukocytes from allergic individuals who had positive skin tests and negative RAST released a significant amount of histamine upon challenge with specific allergen as well as antihuman IgG4, suggesting that this immunoglobulin is a functional component of some immediate hypersensitivity reactions.

Adult↗
PubMed1978

A study on the IgE levels of military recruits and association with HLA antigens.

To identify antigen marker(s) on lymphocytes for the immunoregulation of IgE immunoglobulin. For this purpose, IgE determinations were performed on 58 recruits who were arbitrarily selected for HLA typing. Thirty-three recruits with IgE values ranging from 24 to 142 U/ml (71 U/ml (71 U/ml average) showed a slight increase in incidence for HLA-A23, -A33 and BW-15 when compared to a group of 25 recruits whose IgE values ranged from 153 to 2128 U/ml (615 U/ml average). Examination for C-loci specificity indicated that CW-1, CW-3 and CW-4 similar for both groups; however, the "low" IgE group had a frequency of 55.5% of recruits possessing CW-2 antigen compared with only 15% for the "high" group. The value for the "high" IgE appears to be similar to that of the normal population (10% incidence) and only slightly elevated when compared to 7% (2/29) found when the identical CW-2 antisera were tested with laboratory personnel. Thus the associations of HLA-CW2 with the recruits within the "low" IgE group suggests that this HLA marker may be an expression for a suppressed response for IgE immunoglobulin. Viral and bacterial infections, found frequently in these recruits, are discussed as a possible cause of this suppression.

HLA Antigens↗
PubMed1978

Viral infections and IgE levels.

Six non-atopic patients with respiratory infections to a variety of viruses demonstrated a consistent drop in IgE levels (35% to 87%) in the convalescent phase compared to the acute phase of infection, suggesting that the viral agent affects T lymphocytes to suppress and hence B lymphocytes to secrete IgE immunoglobulin.

Asthma↗
PubMed1977

Allergy testing.

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Animals↗
PubMed1977

Inhibition of reagin-mediated PCA reactions in monkeys and histamine release from human leukocytes by human IgG4 subclass.

Human myeloma proteins of IgG4 subclass in contrast to myeloma proteins IgG1, IgG2 and IgG3, were capable of blocking PCA reactions in monkeys mediated by human reaginic antibodies of IgE class. In addition to IgE, IgG4 myeloma protein was also capable of sensitizing leukocytes from normal individuals and gave histamine release (HR) upon challenge with anti-human IgG4. Leukocytes from 11 allergic individuals and from 9 normal subjects sensitized with the serum of allergic patients, were capable of releasing histamine with anti-human IgG4, anti-human IgE, and the specific allergen. No response was obtained with anti-human IgG1 and IgG3 sera. Leukocytes from the normal individuals released histamine from 3 to 20% with anti-human IgG4 and from 6 to 30% with anti-human IgE. Moreover, normal leukocytes sensitized with IgG4 myeloma protein or a serum of an allergic patient heated at 56 degrees C for 2 h, released a significant amount of histamine on challenge with anti-human IgG4 whereas no response was obtained with anti-human IgE. The biological role of human IgG4 in immediate hypersensitivity reactions is discussed in relation to human IgE.

Animals↗
PubMed1976

An electron microscope study of receptor site on rat mast cells for human immunoglobulin E.

Previous studies using immunofluorescence and autoradiography demonstrated the presence of specific immunoglobulin E (IgE) receptors on rat mast cells (RMC). This finding was confirmed by the use of transmission electron microscopy (TEM) combined with X-ray microanalysis (XMA) employing ferritin labelled antisera XMA was used to detect ferritin as Fe in the target cells. On examination by TEM and XMA, 25 per cent of RMC showed a dense area of Fe deposits on the surface of the cells. No Fe could be detected on neutrophils or eosinophils or in the control cell preparations without human IgE. The Fe was concentrated in well-defined areas of the treated mast cells and this distinct concentration of receptor sites was similar to that observed by other investigators who have termed this phenomenon, "cap" formation.

Animals↗
PubMed1975

Production of rat homocytotropic antibodies using low dose, long term oral exposure to penicillin G.

Sera from 15 patients with immediate hypersensitivity reactions to penicillin G gave positive responses in the rat mast cell test (RMCT) indicating the presence of IgE-type antibodies in the sera. Five sera were from patients who had had reactions to penicillin 15 to 22 years previously without known re-exposure to this antigen. To explore the possibility that non-therapeutic exposure to penicillin may have produced continued sensitization in these patients, an animal model system was developed to explore the efficacy of low dose, long term oral exposure to penicillin G in rats for producing homocytotropic antibodies in these animals. It was found that when rats were given penicillin G in their drinking water at concentrations of 0.1 to 1 U/ml over a period of 1 to 3 months they produced serum IgE and IgGa antibodies. In addition, IgE antibodies were actively bound to the peritoneal mast cells of these animals. The presence of circulating or cell bound antibodies was detected using the rat mast cell test. It was also shown that rats given penicillin G orally for 1 month were more prone to antibody production after a single intramuscular injection of penicillin G compared to a control group receiving only the intramuscular injection of this antigen. The results of this study are discussed in terms of possible non-therapeutic sensitization towards penicillin G in the human population.

Administration, Oral↗
PubMed1975

Production of anaphylactic antibodies to drugs in experimental animals. I. Benzylpenicillin.

Studies were undertaken to determine if animals exposed to benzylpenicillin could produce anaphylactic antibodies to the drug moiety. For this purpose, Albino Wistar rats were divided into four groups. Two groups of animals were immunized with benzylpenicillin using either the intraperitoneal or the subcutaneous route. The remaining two groups were used for feeding experiments. The animals were bled upon termination of the experiments; their sera were collected and pooled per group. The pooled sera were tested for the presence of anaphylactic antibodies. Histamine release from rat mast cells under the appropriate conditions was used as an indicator of antibody production. Penicilloylpolylysine and benzylpenicillin were both employed to determine antibody specificity. It was found that sera from all groups of animals contained anaphylactic antibodies with specificity towards the penicilloyl group and "benzylpenicillin".

Administration, Oral↗
PubMed1975

Lymphocytotoxins and mixed leucocyte culture blocking factor activity in the plasma of uraemic patients undergoing haemodialysis.

Mixed leucocyte culture blocking factor activity (MLC-BFA) in the plasma of haemodialysis patients appears as a result of recent blood transfusions and is concentrated in IgG fractions of the blocking serum. Four patients neither developed lymphocytotoxins nor MLC-BFA in spite of having received multiple blood transfusions. Such 'unresponsive' recipients had an excellent clinical course upon receiving an allograft.

Blood Transfusion↗