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Biomedical subjects

L Pereira

Publications and source records attributed to L Pereira.

161 records · Page 9Linked to original sources
PubMed1980

Type-common and type-specific monoclonal antibody to herpes simplex virus type 1.

Hybridoma cells produced by fusing mouse myeloma cells with spleen cells from mice primed with herpes simplex virus (HSV) type 1 yielded five clones producing neutralizing antibody against homologous virus. Two clones, HCl and HC2, produced antibody capable of precipitating glycoprotein C and its precursor, whereas three clones, HD1, HD2, and HD3, produced antibody capable of precipitating glycoprotein D and its precursor. Antibody produced by the HC1 and HC2 clones neutralized HSV type 1 but not HSV type 2 or HSV type 1 strain MP, which is known to lack glycoprotein C. Antibody produced by the HD1 and HD2 clones neutralized both HSV type 1 and HSV type 2, whereas antibody produced by the HD3 clone neutralized HSV type 1 but not HSV type 2. The two clones which produced antibody to glycoprotein C and the two clones which produced type-common antibody to glycoprotein D were independently derived and not clonally related inasmuch as the antibody in each pair belonged to a different subclass of immunoglobulin.

Animals↗
PubMed1978

Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 X HSV-2 recombinants.

In an earlier paper (Morse et al., J. Virol 24:231--248, 1977) we reported on the provenance of the DNA sequences in 26 herpes simplex virus type 1 (HSV-1) X HSV-2 recombinants as determined from analyses of their DNAs with at least five restriction endonucleases. This report deals with the polypeptides specified by the recombinants and by their HSV-1 and HSV-2 parents. We have identified (i) the corresponding HSV-1 and HSV-2 polypeptides with molecular weights ranging from 20,000 to more than 200,000, (ii) the polypeptides that undergo rapid post-translational processing, and (iii) polypeptides that vary intratypically in apparent molecular weight. By comparing the segregation patterns of the polypeptides with those of the DNA sequence of the recombinants, we have mapped the templates specifying 26 polypeptides and several viral functions on the physical map of HSV DNA. The data show the following: (i) alpha polypeptides map at the termini of the L and S components of the HSV DNA. Although alpha ICP 27 maps entirely within the reiterated region of the L component, the template for alpha ICP 4 may lie only in part within the reiterated sequences of the S component. Of note is the finding that cells infected with a recombinant that contains both HSV-1 and HSV-2 DNA sequences in the S component produced alpha ICP 4 of both HSV-1 and HSV-2. (ii) Templates specifying beta and gamma polypeptides map in the L component and appear to be randomly distributed. (iii) Thymidine kinase and resistance to phosphonoacetic acid mapped in the L component. In addition, we have taken advantage of the rapid inhibition of host protein synthesis characteristic of HSV-2 infections and syncytial plaque morphology to also map the template(s) responsible for these functions in the L component. The implications of the template arrangement in HSV DNA are discussed.

Base Sequence↗
PubMed1978

The use of intertypic recombinants for analysis of gene organization in herpes simplex virus.

Analysis of the DNA sequence arrangement and polypeptides specified by 28 HSV-1 x HSV-2 recombinants show the following: (i) Recombinants with heterogeneous L and S components or with heterogenous inverted repeats are viable. (ii) HSV-1 and HSV-2 genes appear to be functionally equivalent and with few exceptions co-linearly arranged. Co-linear DNA maps have been established. (iii) At most two arrangements of HSV DNA are capable of replication. This is consistant with current studies suggesting that sequence arrangements are the consequence of obligatory post-synthesis repair. (iv) alpha Polypeptides map at the termini of the L and S components of HSV DNA. Although alpha ICP 27 maps entirely within the reiterated region of the L component, the template for alpha ICP 4 may lie only in part within the reiterated sequences of the S component. Of note is the finding that cells infected with a recombinant that contains both HSV-1 and HSV-2 DNA sequences in the S component, produced alpha ICP 4 of both HSV-1 and HSV-2. (v) Templates specifying beta and gamma polypeptides may in the L component and appear to be randomly distributed. (vi) The genes specifying thymidine kinase, resistance to phosphonoacetic acid and syncytial plaque morphology mapped in the L component. In addition, we have taken advantage of the rapid inhibition of host protein synthesis to map the gene(s) specifying this inhibition in the L component.

Chromosome Mapping↗
PubMed1976

Variability in the structural polypeptides of herpes simplex virus 1 strains: potential application in molecular epidemiology.

This paper reports on the variability of structural polypeptides of 53 strains of herpes simplex virus 1 isolated from Italy, Uganda, South Africa, and various locations in the United States. Most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numberous passages at variable multiplicities in cell culture and experimental animals. The acrylamide gel electrophoresis of polypeptides from purified virions revealed seven variable polypeptides. Virion polypeptides (VP) 7, 11, 13, 14, 15.2 and 23 were present in at least two isotypic forms characterized by fast and slow electrophoretic mobilities. VP8 could not be detected in three strains. In addition, VP13, 15.2, and 23 in some strains were either absent or comigrated with other polypeptides. A variety of tests showed that the variability in electrophoretic mobility of polypeptides was reproducible and could not be attributed to artifacts of purification, solubilization, or electrophoresis. Attempts to classify the strains on the basis of electrophoretic mobility of five or all seven variable polypeptides yielded 14 and 19 groups, respectively. The bulk of the strains (41 to 53) fell into six groups. Not all possible permutations of variable polypeptides were observed. Comparison of early and late passages of laboratory strains showed that in the few instances tested the variability could not be attributed to the propagation of the virus outside the human host. Clustering of strains on the basis of country of origin was not observed. Some clustering of isolates on the basis of site of isolation was observed, and the data do suggest that further analyses of isolates for evidence of a correlation between the site of localization on the human body and the structural polypeptides might be useful. Electrophoretic characterization of structural polypeptides has the potential of becoming a powerful tool for epidemiological studies of herpes simplex virus infections.

Electrophoresis, Polyacrylamide Gel↗
PubMedDate not supplied

Prevalence of lower genital tract infections in pregnancy.

A descriptive profile of 106 pregnant women with various forms of cervicitis and vaginitis is provided. Fifty women attending individual physicians' private offices are compared with 56 clinic patients: 34 attending a university prenatal teaching clinic and 22 attending a pregnancy termination unit. Univariate analysis showed that single women were significantly more likely to be infected by mycoplasmas, yeasts, trichomonads, and Gardnerella vaginalis than were married women. Teenagers were more frequently infected by Mycoplasma hominis, yeasts, and Trichomonas vaginalis than were women older than 20 years. After stepwise logistic regression analysis, the most significant predictor of infection with M. hominis, Ureaplasma urealyticum, or yeasts was being a clinic patient; for G. vaginalis, the most significant variable was being unmarried. These data suggest that teenaged and single women who are pregnant will benefit most from routine screening for vaginitis/cervicitis-producing microorganisms.

Adolescent↗
PubMedDate not supplied

[Aneurysm of the Valsalva sinus. Echocardiographic diagnosis. Apropos of a case of long post-rupture survival].

The authors present a case of aneurysm of the right sinus of Valsalva with rupture and aorto-cardiac shunt to the exit chamber of right ventricle. Based on clinical information, one can assume that it could be a case which has been with few symptoms for a period of seven years or more. The diagnosis was made possible due to the images obtained by M mode and two dimensional echocardiography, which allowed an unmistakable view of an aneurysmatic dilatation and the site of the rupture which was confirmed later by Doppler echocardiography with colour codification and and anatomical assessment at surgery.

Adult↗