Search PubMed⌕ Search

Biomedical subjects

L Pearson

Publications and source records attributed to L Pearson.

At least 73 records · Page 4Linked to original sources
PubMed1985

Remitting seronegative symmetrical synovitis with pitting edema. RS3PE syndrome.

Eight elderly men and two elderly women presented with symmetrical polysynovitis of acute onset involving most of their appendicular joints and flexor digitorum tendons associated with pitting edema of the dorsum of both hands and both feet. Onset of seven of the ten cases could be pinpointed almost to the hour. Rheumatoid factors were absent from serum samples in all, and no radiologically evident erosions developed. Clinical and laboratory signs of inflammation and the edema disappeared gradually in each case. Treatment consisted of aspirin or other nonsteroidal anti-inflammatory drugs. Hydroxychloroquine, 200 to 400 mg/day, was given in six and gold therapy in two cases. Painless limitation of motion of the wrists and/or fingers persisted in all, although the patients were both unaware of and unhampered by this abnormality. Six of eight cases where typing was possible were positive for HLA-B7, CW7, and DQW2 (relative risk for B7, 9.5). Three cases of this syndrome were found in a consecutive series of 52 men diagnosed as having definite "rheumatoid arthritis," and thus represent a distinctive condition with an excellent prognosis.

Acute Disease↗
PubMed1985

The use of pNJ5000 as an intermediate vector for the genetic manipulation of Agrobacterium Ti-plasmids.

The use of broad-host-range plasmids derived from RP4 as intermediate vectors for the transfer of narrow-host-range recombinant plasmids from Escherichia coli to Agrobacterium tumefaciens as a preliminary to marker exchange is described. Recombinant plasmids having a ColE1 type origin were linked to the RP4 derivative. Cointegrate formation appeared to take place by RecA-independent, homologous recombination within a short piece of DNA derived from the beta-lactamase gene of Tn1/Tn3 carried by both vector components, so that it never disrupted the recombinant portion of the construction. pNJ5000 provides an unstable intermediate vector for use in marker exchange experiments, while its stable relative pNJ1020 provides a carrier for use in binary vector systems.

Cloning, Molecular↗
PubMed1983

The role of cytosine methylation in the control of nopaline synthase gene expression in a plant tumor.

The FT37/1 tumor line induced by Agrobacterium tumefaciens on flax epicotyls contains 22-24 copies of the T-DNA encoded nopaline synthase gene per cell. All the gene copies are methylated to some extent but the methylation is not uniform, nor does it reflect the methylation level of the flanking plant DNA. This extensive methylation correlates with an extremely low level of expression of the nopaline synthase gene. Treatment of the tumor line with the in vivo demethylating drug 5-azacytidine at a concentration of 3 X 10(-5) M, resulted in the demethylation of, on average, one copy of the nopaline synthase gene per cell. This demethylation was paralleled by an increase in the transcription of the gene and indicates that cytosine methylation is capable of suppressing the expression of plant genes in vivo.

Amino Acid Oxidoreductases↗
PubMed1982

Prophylactic lymph node dissection in clinical stage I cutaneous malignant melanoma: results of surgical treatment in 1319 patients.

A retrospective study was made of 1319 patients with cutaneous primary malignant melanoma and regional lymph nodes clinically free of disease, whose first definitive surgical treatment was either (a) wide excision of the primary lesion (WE) or (b) wide excision of the primary lesion plus a prophylactic lymph node dissection (PLND). Patients were stratified according to three dominant prognostic factors, as revealed by multifactorial analyses: sex of patient, the anatomical site and thickness of the primary lesion. Of 380 patients who underwent PLND for lymph nodes clinically free of disease, there was microscopic evidence of nodal metastases in 5 per cent. Despite this, prognosis in these patients was nearly twice as good as in those patients undergoing lymph node dissection for clinically involved nodes. Five-year survival rates for all those clinical stage I patients with lesions less than 0.8 mm thick were high irrespective of initial surgical treatment. In men with lesions of intermediate thickness (particularly 1.6-3.0 mm), survival rates for those undergoing PLND were considerably higher than for those undergoing WE. There were no survival benefits of PLND in men with lesions thicker than 3.0 mm. This was in contrast to the situation in women, where PLND was indicated for patients with the thickest lesions. This study has defined three parameters which can serve as useful practical guides for selecting alternative surgical procedures in the management of clinical stage I cutaneous malignant melanoma.

Adult↗
PubMed1982

Climacteric.

Explore the source record for details and available documents.

Adult↗