Biomedical subjects

L Pays

Publications and source records attributed to L Pays.

8 recordsLinked to original sources

PubMed2002

Serine protease inhibitor Spi2 mediated apoptosis of olfactory neurons.

The olfactory epithelium of adult mouse, where primary sensory neurons are massively committed to apoptosis by removal of their synaptic target, was used as a model to determine in vivo mechanisms for neuronal cell death induction. A macro-array assay revealed that the death of olfactory neurons is accompanied with over-expression of the serine protease inhibitor Spi2. This over-expression is associated with decreased serine protease activity in the olfactory mucosa. Moreover, in vitro or in vivo inhibition of serine proteases induced apoptotic death of olfactory neuronal cells. Interestingly, Spi2 over-expression is not occurring in olfactory neurons but in cells of the lamina propria, suggesting that Spi2 may act extracellularly as a cell death inducer. In that sense, we present evidence that in vitro Spi2 overexpression generates a secreted signal for olfactory neuron death. Hence, taken together these results document a possible novel mechanism for apoptosis induction that might occur in response to neurodegenerative insults.

Animals↗

PubMed2000

Semaphorin 3A is required for guidance of olfactory axons in mice.

Semaphorin 3A (Sema3A) is a membrane-associated secreted protein that has chemorepulsive properties for neuropilin-1 (npn-1)- expressing axons. Although mice lacking the Sema3A protein display skeletal abnormalities and heart defects, most axonal projections in the CNS develop normally. We show here that Sema3A is expressed in the lamina propria surrounding the olfactory epithelium (OE) and by ensheathing cells in the nerve layer of the ventral olfactory bulb (OB) throughout development. Subsets of sensory neurons expressing npn-1 are distributed throughout the OE and extend fibers to the developing OB. In wild-type mice, npn-1-positive (npn-1(+)) axons extend to lateral targets in the rostral OB and medial targets in the caudal OB, avoiding regions expressing Sema3A. In Sema3A homozygous mutant mice, many npn-1(+) axons are misrouted into and through the ventral nerve layer, beginning as early as embryonic day 13 and continuing at least until birth. At postnatal day 0, npn-1(+) glomeruli are atypically located in the ventral OB of Sema3A(-/-) mice, indicating that aberrant axon trajectories are not corrected during development and that connections are made in inappropriate target regions. In addition, subsets of OCAM(+) axons that normally project to the ventrolateral OB and some lactosamine-containing glycan(+) axons that normally target the ventral OB are also misrouted in Sema3A mutants. These observations indicate that Sema3A expression by ensheathing cells plays an important role in guiding olfactory axons into specific compartments of the OB.

Animals↗

PubMed2000

Patterning of olfactory sensory connections is mediated by extracellular matrix proteins in the nerve layer of the olfactory bulb.

In early rat embryos when axons from sensory neurons first contact the olfactory bulb primordium, lactosamine-containing glycans (LCG) are detected on neurons that are broadly distributed within the olfactory epithelium, but that project axons to a very restricted region of the ventromedial olfactory bulb. LCG(+) axons extend through channels defined by the coexpression of galectin-1 and beta2-laminin. These two extracellular matrix molecules are differentially expressed, along with semaphorin 3A, by subsets of ensheathing cells in the ventral nerve layer of the olfactory bulb. The overlapping expression of these molecules creates an axon-sorting domain that is capable of promoting and repelling subsets of olfactory axons. Specifically, LCG(+) axons preferentially grow into the region of the nerve layer that expresses high amounts of galectin-1, beta2-laminin, and semaphorin 3A, whereas neuropilin-1(+) axons grow in a complementary pattern, avoiding the ventral nerve layer and projecting medially and laterally. These studies suggest that initial patterning of olfactory epithelium to olfactory bulb connections is, in part, dependent on extracellular components of the embryonic nerve layer that mediate convergence and divergence of specific axon subsets.

Age Factors↗

PubMed2000

Gal-NCAM is a differentially expressed marker for mature sensory neurons in the rat olfactory system.

A new monoclonal antibody, 2E11, was produced by immunizing mice with the microsomal fraction of rat accessory olfactory bulb cells. This IgM recognizes a previously described complex alpha-galactosyl containing glycolipid, as well as N-linked glycoproteins at 170 and 210 kD. These proteins correspond to a new nerve cell adhesion molecule (NCAM) glycoform, Gal-NCAM, which contains a blood group B-like oligosaccharide. During embryonic development, the 2E11 epitope is expressed by a subset of mature olfactory sensory neurons randomly dispersed throughout the olfactory epithelium, whereas in the olfactory bulb, immunostaining is restricted to medial areas of the nerve layer. When compared to PSA-NCAM, another NCAM glycoform, Gal-NCAM has a mutually exclusive distribution pattern both in the olfactory epithelium and in the olfactory bulb. We propose a model for the hierarchy of neuronal maturation in the olfactory epithelium, including a switch from PSA-NCAM expression by immature neurons to the expression of Gal-NCAM by mature neurons.

Animals↗

PubMed1997

Close link between cutaneous nerve pattern development and feather morphogenesis demonstrated by experimental production of neo-apteria and ectopic feathers: implication of chondroitin sulphate proteoglycans and other matrix molecules.

In chick skin, nerve arcades develop around the base of feathers. In order to understand the mechanisms of their formation, we have tried to dissociate arcade formation from feather morphogenesis in various ways. Nerve patterns were analysed (1) in hydrocortisone-treated embryos that are partially devoid of feathers, (2) after retinoic acid treatment that produces ectopic feathers, (3) in dorsal root ganglia-skin co-cultures. Whenever tested, immunochemistry revealed that nerve arcades form around chondroitin sulphate proteoglycan-rich areas. Hydrocortisone treatment modifies the distribution of two out of three chondroitin sulphate proteoglycan epitopes tested, as well as the shapes of the feathers and nerve arcades, but not fibronectin, tenascin or laminin localizations. Chondroitinase digestion in co-cultures eliminated the nerve arcade formation and produced abnormally thin feathers, but nevertheless with a normal spatial distribution. Thus, chondroitin sulphate proteoglycans are probably not involved in the overall arrangement of feathers, but appear to play a fundamental role in both the formation of nerve arcades and the morphogenesis of the feather.

Animals↗

PubMed1997

Regulation of the chick cutaneous innervation pattern in retinoic acid-induced ectopic feathers and in the naked neck mutant.

In chick skin, nerve fibers develop in a typical network formed by arcades around the base of feathers. In this study, we tried to dissociate the morphogenesis of nerve arcades and feathers, and to clarify the implication of several matricial molecules in these two developmental events. For this purpose, cutaneous nerve pattern and distribution of fibronectin, tenascin, and three epitopes of chondroitin sulfate proteoglycans (CSPGs) have been immunohistologically studied in the skin of the specific apteria of naked neck chick mutants, which lack feathers in the neck area, and in the tarso-metatarsal zone of retinoic acid-treated embryos where ectopic feathers grow. The presence of feathers was always associated with nerve arcades; no arcades were present in featherless areas. Specific immunofluorescence for tenascin and two epitopes of CSPGs revealed different distributions in the naked-neck neo-apteria as compared to control apteria. Moreover, the only difference in matricial composition in ectopic feathers concerned a CSPG isoform, bringing additional evidence that extracellular matrix molecules, and especially some (but not all) CSPGs, are involved both directly and indirectly in the cutaneous nerve pattern development.

Animals↗

PubMed1996

[Development of the cutaneous nervous system].

Skin of vertebrates is richly innervated, mainly by sensory nerve fibres which form a well organized pattern, particularly around phaners. This innervation develops segmentally (dermatomes) from cutaneous branches provided by spinal nerves. The innervation begins at 13 days (E 13) in the mouse embryo and, although hair buds form at E 16, follicles are only innervated from 5 days postnatally being complete at about 20 days. In the chick skin, innervation forms a regular and characteristic pattern around feathers, and can be visualized on whole mounts. Its development can be traced from 6 days of development in relation to feather morphogenesis. Experiments producing non formation of spinal ganglia (X-ray irradiation or neural tube ablation) or production of neoapteria (hydrocortisone treatment) or ectopic feathers on scales (retinoic acid treatment) show there is a close link between feather development and nerve pattern formation. In vitro co-cultures of dorsal root ganglia and epidermis combined with the use of synthesis inhibitors and antibodies, showed that epidermis has a repulsive effect on nerve fibres mediated, at least in part, by chondroitin sulphate proteoglycans. These compounds have been localized, using antibodies mainly at the base of the feather buds and seem to play a key role in the construction of the fine nerve pattern around feather follicles. In conclusion, the specific nerve patterns are the final result of selective responses of growing nerve endings to unique combinations of local cues and conflicting interactions which are developmentally regulated in parallel with the morphogenesis of phaners.

Aggrecans↗

PubMed1994

Development of sensory innervation in chick skin: comparison of nerve fibre and chondroitin sulphate distributions in vivo and in vitro.

In bird skin, nerve fibres develop in the dermis but do not enter the epidermis. In co-cultures of 7-day-old chick embryo dorsal root ganglia and epidermis, the neurites also avoid the epidermis. Previous studies have shown that chondroitin sulphate proteoglycans may be involved. Chondroitin sulphate has therefore been visualized by immunocytochemistry, using the monoclonal antibody CS-56, both in vivo and in vitro using light and electron microscopy. Its distribution was compared to those of 2 other chondroitin sulphate epitopes and to that of the growing nerve fibres. In cultures of epidermis from 7-day-old embryonic chicks, immunoreactivity is found uniformly around the epidermal cells while at 7.5 days the distribution in dermis is heterogeneous, and particularly marked in feather buds. In vivo, chondroitin sulphate immunoreactivity is detected in the epidermis, on the basal lamina, on the surfaces of fibroblasts and along collagen fibrils. This localization is complementary to the distribution of cutaneous nerves. Chondroitin sulphate in the basal lamina could prevent innervation of the epidermis and the dermal heterogeneities could partly explain the nerve fibres surrounding the base of the feathers. Chondroitin sulphate could therefore be important for neural guidance in developing chick skin.

Animals↗