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Biomedical subjects

L Pajor

Publications and source records attributed to L Pajor.

At least 73 records · Page 4Linked to original sources
PubMed1991

Morphologic and flow cytometric analysis of circulating megakaryoblasts in chronic myeloid leukaemia.

The immunophenotype (a), ultrastructural features (b) and cell kinetics (c) of circulating megakaryoblasts have been studied in two cases of pure megakaryoblastic and one case of mixed (myeloblastic, megakaryoblastic) cell proliferation in chronic myeloid leukaemia (CML). (a) The blast cells showed early megakaryocyte differentiation antigen (HLA-DR), platelet specific GpIIIa (CD61) and GpIIb-IIIa (CD41) antigens in different percentages. (b) The megakaryoblasts were recognized by the presence of platelet GpIIIa (CD61) demonstrated by an immunoelectron microscopic method. The labelled cells were "lymphocyte-like" megakaryoblasts and cells with features of cytoplasmic maturation (demarcation membranes, alpha granules and vacuoles). (c) Cellular DNA content of the megakaryoblasts was measured by propidium iodide (PI) staining of cells expressing platelet GpIIIa (CD61). Flow cytometric (FC) DNA analysis revealed no aneuploidy and high ploidy (greater than 4N) cell population. In the two cases of pure megakaryoblastic proliferation a high percentage of the megakaryoblasts were in the S-phase, while the non-megakaryoblastic cell fraction showed no elevated S-phase compartment. It is concluded that in CML the circulating megakaryoblasts (1) have a nuclear maturation arrest and accumulation at the level of tetraploid DNA content, (2) surface antigen expression and cytoplasmic organelles show a tendency to mature and (3) in pure megakaryoblastic proliferation the myeloid cells are not in the cell compartment showing high proliferation.

Antigens, CD↗
PubMed1991

Intraoperative injuries during transperitoneal urological operation.

The mechanism of development of 19 severe side-injuries arising during 230 transperitoneal urological operations was analysed. During a total of 158 RLAs performed for testicular tumour, vascular injuries--of the aorta, inferior caval vein and renal vessels--were suffered in 10 cases, and injury of the small intestine in one case. In another case, occidental ligation and transection of the ureter were made. During radical nephrectomy, liver and splenic injuries were inflicted in two cases each. The left renal vein was injured also in two cases. In one case the contralateral ureter was ligated. Disregarding the first five cases, the injuries were always made in cases of tumours. After analysing the mistakes, suggestions were put forward for eliminating these complications.

Blood Vessels↗
PubMed1991

Cholesteryl hemisuccinate's inductive effect on membrane rigidization regarding both, its remodelling of the cells' surface receptor pattern and its decreasing the natural killer susceptibility of K-562 cells.

The relationship of changes in membrane fluidity to natural killer susceptibility of K-562 target cells was investigated. Membrane rigidization was performed by the chemical modulator cholesteryl hemisuccinate. Steady-state fluorescence polarization measurements of the diphenyl hexatriene labelled, modified K-562 cells revealed that cholesteryl hemisuccinate increased the structural order of the hydrophobic region of membranes in a dose dependent way. Investigation of natural killer susceptibility followed by 51Cr release assay indicated that modified cells are less sensitive to natural killer attack. To elucidate whether surface structures such as transferrin and lectin receptors are associated with the altered susceptibility, the surface density of these receptors was followed by (I-125)-transferrin binding assay and quantitative immunofluorescence. We found that the number of transferrin and concanavalin A receptors increased by a factor of 2.44 and 2.00, respectively, whereas that of the wheat germ agglutinin receptor failed to exhibit any changes upon rigidization. From the results we concluded that i the membrane structural order does influence the natural killer susceptibility, ii changes in membrane structural order result in alteration of the number of cell surface transferrin and lectin receptors, iii however, no direct relationship seems to exist between these two events.

Cholesterol Esters↗
PubMed1990

Radical prostatectomy and complications.

Radical prostatectomy has been successfully applied in the treatment of intracapsular prostatic tumour. A few modifications of the surgical technique are suggested to prevent complications. Initial results confirm that the method works, as all patients have remained continent. One of them died of pulmonary embolism right in the postoperative phase.

Humans↗
PubMed1990

[Malignant lymphomas: pathomorphology and experiment].

The last fifteen years have provided a significant development in pathomorphological diagnosis of malignant lymphomas (ml), in clinical fellow-up of patients and in its therapy. Diagnosis is built on functional morphological basis (immunohistology). New methods have reslited in identification of new types of mls. On the basis of high number of cases registered in Lymphoma Reference Centre, specific organic distribution new entities can be determined. Perifollicular B-cellular mls of MALT type are frequent in gastrointestinal tract and are rare in Waldeyer ring and in lymph nodes. Flow cytometric determination of DNA content of cells proved to be very useful in diagnostics of mls, so the probably determination of aneuploid and proliferative activity, respectively. These parameters are of prognostic significance. Recirculation of lymphocytes may play a role in dissemination os nHmls. It can be studied in immunomorphological (in vitro) test ased on the interaction (adherence) of lymphocytes and of endothelial cells of high endothelial postcapillary venule. Our observations present that B-cellular CLL and clonal cells of plasma cellular leukemia show endothelial adherence, while multiple cells of myeloma do not.

Animals↗
PubMed1989

Radical nephrectomy through total median laparotomy.

Twenty-two carcinoma patients aged less than 65 years were subjected to radical nephrectomy through total median laparotomy. This type of approach provides excellent access to both essential operation phases: vessel management and lymphadenectomy. Postoperative abdominal complications and hernia have not been encountered.

Adult↗
PubMed1989

Multiple urolithiasis in bilharziasis patients.

A total of 780 patients were operated on for lithiasis. Among them 97 presented multiple urolithiasis which was often associated with Schistosoma infection. Bilharziasis, in accord with theoretical considerations, plays a part in the formation of calculi, especially in that of multiple urolithiasis.

Cross-Sectional Studies↗
PubMed1989

The relationship of membrane fluidity to growth and nuclear cycle in mitogen stimulated lymphocytes.

Fluorescence polarization (P) and anisotropy (rs) of the diphenyl-hexatriene labeled, stimulated human lymphocytes were followed in relation to cell cycle progression. Fluorescence anisotropy is proportional to the structural order parameter of the apolar lipid region, thus, indicative of the membrane fluidity. Cell cycle progression was defined by monitoring the RNA, protein and DNA synthesis as well as the expression of interleukin 2 and transferrin receptors. Cellular growth in size was also measured. A decrease in P and rs values was seen when the RNA and protein synthesis as well as the density of growth factor receptors and cellular volume increased. At the same time no DNA synthesizing cell was detected. The low P and rs values remained unaltered throughout the cycle, thus proving to be independent from cell proliferation (DNA synthesis) and from further progressive increase in cellular volume. The data indicate that the structural change in the membranes is an early event of cell activation and occurs in the course of G0-G1 transition as part of the growth cycle.

Cell Cycle↗
PubMed1989

Schistosomal granulation masquerading as testicular tumour.

The case of a 7-year-old boy is reported in whom the Schistosoma haematobium infection manifested as the clinical picture of metastasizing testicular tumour. The correct diagnosis was established by the postoperative pathohistological examination. No similar observations was found in the literature studied.

Child↗
PubMed1989

The effectiveness of venous hypothermia in the left kidney.

Twenty renal parenchyma operations of the left kidney were performed in venous hypothermia. The hypothermic solution was perfused via the spermatic (ovarian) vein, outflow occurred through the vessels opened by the incision. The effectiveness of venous hypothermia was measured by the probe of an electrical thermometre inserted into the renal tissue. The temperature of the cooled kidney became constant between 20 and 23 degrees C by using a perfusion solution of 4-5 degrees C. The method can be used for its simplicity and safety also in extensive renal parenchyma operations, its only limitation is that it can be performed only on the left kidney.

Humans↗
PubMed1989

[Successful surgical treatment of masculine pseudohermaphroditism].

It is reported on the successful surgical therapy of a male pseudohermaphrodit. The transformation to a woman was made in two stages. In the first session castration and penile amputation were performed and in the second operation a vaginal reconstruction using a small bowel segment has been done.

Adolescent↗
PubMed1988

Comparison of membrane fluidity and transferrin receptor expression as proliferation markers in lymphoproliferative disorders and in mitogen induced lymphoblasts.

The correlation of two proliferation related events, membrane fluidization and transferrin receptor expression was studied in different lymphoid cells. With increasing grade of malignancy the membrane microviscosity decreased. Moreover, resting lymphocytes differed significantly from chronic lymphocytic leukaemia cells in this parameter. [125I]Fe2-transferrin binding increased with increasing proliferation capacity of cells. The membrane microviscosity of mitogen induced blasts was very similar to that of acute lymphoblastic cells, whereas the [125I]Fe2-transferrin binding capacity exceeded roughly 5 times that of the blastic leukaemia cells. This dissociation indicates that these two proliferation markers do not necessarily parallel each other.

Cell Division↗
PubMed1988

Histogenesis of metastatic tumours: lectin reactive substances and intermediate filament proteins.

The reactivity of 38 anaplastic tumours for cytokeratin, vimentin, desmin, milk fat globule membrane antigen. Ia antigen as well as peanut agglutinin and Ulex europaeus I lectins were investigated immunohistologically in paraffin embedded tissue samples. By this typing of tumours establishment of the histogenesis of 34 out of 38 neoplasms was possible. Among the epithelial markers milk fat globule membrane antigen proved to be superior to cytokeratin. Vacuolar or intraluminal binding of peanut lectin was found to be more sensitive than PAS reaction for detecting early secretory activity. Our study substantiates the previous data that binding of Ulex europaeus I lectin is indicative of nasopharyngeal carcinoma. In this study we also provide evidence for vimentinization in solid metastases as well as for shift in the cytokeratin profile upon malignant transformation.

Carbohydrates↗
PubMed1988

Course and transformation of polycythaemia vera in relation to therapy.

The fate of the polycythaemic patient depends on the treatment employed which may determine the nature of the transformation commonly occurring late in the course of the disease. Treatment is, on the other hand, aimed at prevention of the most frequent complications, that is of thromboembolic processes. In the last 30 years the authors treated a total of 118 PV patients, of whom 60 have died. Initially 32P treatment was applied, which was modified later, because of acute leukaemia that had occurred in 9% of the treated cases, to a single 5 mC 32P+Myelobromol (DBM) treatment. Still later only DBM was administered in the form of stosstherapy (2500 mg per day over a period of 4 days). In the latter two groups, acute leukaemia occurred as few as two cases. The course of untreated polycythaemia vera is characterized by transformation into another myeloproliferative disease. This phenomenon occurs in 50% of the cases on drastic treatment and in patients treated with 32P. Of the patients who were alive when the report was finished 35% had been free of complications, while 5.2% were suffering from chronic granulocytic leukaemia (CGL), 34.5% from sclerotic osteo-myelofibrosis (OMF-SC) and 3.4% from chronic megakaryocytic granulocytiv leukaemia (CMGL). Of the 60 patients having died, 15% had suffered from other complications being predominantly of vascular nature. 11.8% of them died of AML, 10% of CGL, 26.7% of OMF-SC and 26.7% of CMGL. The terminal stage was characterized, in the majority of cases, by blastic crisis. Based on their own results and literary data authors recommend DBM treatment besides the indispensable phlebotomy.

Adult↗
PubMed1986

Microsomal desaturation of stearic acid in relation to lymphocyte activation.

The conversion of stearic acid to oleic acid (delta 9-desaturase) was followed in mouse thymocytes stimulated by either concanavalin A or concanavalin A + interleukin-2 resulting in different rates of cell proliferation. To estimate the plasma membrane turnover of oleic acid as compared to that of a saturated fatty acid, double-label experiments ([14C]oleic acid, [3H]palmitic acid) were performed. Following an inhibition delta 9-desaturase was found to be activated from the fourth hour of stimulation. In the early period of cell activation this process proved to be independent of protein synthesis, whereas in the stage of proliferation it was dependent on it. Increased membrane fluidity in the first 30 min of activation is not likely due to enrichment of oleic acid. Cell proliferation and microsomal desaturation seem to be coupled and an increasing amount of oleic acid is at least one of the factors resulting in increased fluidity of the surface membrane of proliferating cells.

Animals↗