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Biomedical subjects

L Pagani

Publications and source records attributed to L Pagani.

At least 73 records · Page 4Linked to original sources
PubMed1985

Degradation of human fibronectin by strains of E. coli K12 carrying R factors from Klebsiella pneumoniae.

We studied the ability to degrade human fibronectin by strains of E. coli K12 carrying R factors from Klebsiella pneumoniae, previously shown to possess a caseinolytic activity. The preliminary data obtained showed that at least two of these R+ strains are able to degrade fibronectin to small peptides retaining the gelatin-binding site of the molecule. We also found indications of non-fibronectin gelatin-binding polypeptides secreted by the strains tested into the culture broth. The possible significance of this bacterial property as a factor of pathogenicity is discussed.

Carrier Proteins↗
PubMed1985

R factor-mediated adhesiveness to mammalian cells in E. coli K12.

We checked the possible effects of the standard plasmids of known compatibility groups on the ability of three strains of E. coli K12, J62, J53 and C600, to agglutinate human and guinea pig erythrocytes and to adhere to cultures of human epithelial cells. The results obtained showed that under defined experimental conditions one plasmid, R478, from a clinical isolate of Serratia marcescens is able to modulate the adhesive properties of the strain J62 which lacks adhesiveness. On the contrary, the same factor did not alter the ability of strains J53 and C600 to agglutinate erythrocytes and to adhere to human epithelial cells in culture, nor did it induce adhesive properties in wild-type strains of E. coli from clinical isolates that lacked them. This would suggest a possible plasmidic control of chromosomally encoded surface structures that mediated adhesiveness of E. coli to mammalian cells.

Adhesiveness↗
PubMed1985

[Prospective use of immunofluorescence polarization in the study of enzymes modifying aminoglycosides].

A number of assays have been described for aminoglycoside-modifying enzymes; the aim of our investigation was to check the validity of polarized immunofluorescence assay for aminoglycoside enzymes. We have studied three enzymes encoded by R-factors from clinical isolates of Enterobacteriaceae conferring various patterns of aminoglycoside-resistance. The assay resulted valid: this is a simple, rapid and sensitive method.

Aminoglycosides↗
PubMed1985

[The role of beta-lactamases and the permeability barrier in resistance to beta-lactam antibiotics].

The role played by beta-lactamases and by permeability barrier in resistance to beta-lactam antibiotics was investigated in 250 clinical isolates of Enterobacteriaceae. The data obtained showed that in 73 strains (52.9%) was a significant correlation between the specific enzyme activity and resistance, however in 38 strains (27.5%) an essential role was played by permeability barrier because the addition of subinhibitory levels of EDTA produced markedly reduction in the MIC.

Anti-Bacterial Agents↗
PubMed1984

Bovine leukemia virus inhibiting activity in fetal calf serum.

We have obtained a new cell line persistently infected with bovine leukemia virus (BLV). BLV-infected cells can be easily detected by indirect immunofluorescence or immunoperoxidase staining technique. The morphology and number of infected cells seem to be related to serum concentration in culture medium: fetal calf serum (FCS) showed a marked effect on virus growth and virus distribution in cell cultures. All of the commercial lots of FCS tested showed BLV inhibiting activity very similar to that previously described for other viruses.

Animals↗
PubMed1984

Relationship between beta-lactamases production and resistance to beta-lactam antibiotics in clinical isolates of Enterobacteriaceae.

288 clinical isolates of Enterobacteriaceae were assayed for beta-lactamase production in order to assess the role played by beta-lactamases in resistance to beta-lactam antibiotics. The data obtained showed that beta-lactamase production is undoubtedly a fundamental determinant for resistance. In fact, there was a significant correlation between the specific activity of the enzyme and the MIC of the strains considered. However, the role played by permeability barriers and/or other factors should be stressed, especially in determining resistance to cephalosporins. The validity of the nitrocefin test for detection of beta-lactamase production in Enterobacteriaeae from clinical isolates was also investigated.

Anti-Bacterial Agents↗
PubMed1983

A plasmid-linked caseinolytic activity in Enterobacteriaceae.

We have tested for production of caseinolytic activity the strain of E. coli K12 J62 carrying different R factors from various Enterobacteriaceae of clinical isolates. The results obtained showed the presence of a caseinolytic activity in 7 out of 139 R factors tested. All of these positive (cas+) R factors derived from strains of Klebsiella pneumoniae and all were able to transfer a strong lac+ phenotype as well as multiple antibiotic resistances (R-lac factors). Another strain of E. coli K12, J53, which is plasmid-free, produced levels of caseinolytic activity comparable to those of the Klebsiella R factors; J62, on the contrary, appeared to possess an inhibitor specific for the caseinolytic activities of J53 and of the R factors but not of other cas+ Gram+ and Gram- organisms. The hypothesis that the expression of the cas+ phenotype in E. coli K12 may be due to the presence of plasmid-linked regulatory genes is discussed.

Caseins↗
PubMed1983

[Epidemiologic observations on Chlamydia trachomatis infections].

A group of 82 patients (77 with urethritis) was studied for the presence of Chlamydia trachomatis infection. The agent was isolated by inoculation on McCoy cell cultures, followed by Giemsa and/or Lugol-iodine staining. The results obtained showed that the frequency of Chlamydia trachomatis infection in the group of patients considered was comparable with that previously reported on other groups. Male patients showed a higher infection rate than females. Different percentages of positivity were obtained in patients with gonococcal infection and in those with post-gonococcal urethritis.

Chlamydia Infections↗
PubMed1981

The prolonged Q-T syndrome presenting as a focal neurological lesion.

A patient with a prolonged electrocardiographic Q-T interval suddenly developed left hemiplegia after an episode of ventricular fibrillation. A CT scan showed a cerebral infarction in the right internal capsule. The prolonged Q-T interval, by virtue of its association with cardiac arrhythmias, may cause cerebral hypoperfusion that commonly results in generalized neurological deficits. The lack of evidence for embolization in this patient suggests that decreased cerebral perfusion was responsible for the genesis of her neurological signs and symptoms. The prolonged Q-T interval is an easily overlooked cardiac abnormality that must be considered in a patient who exhibits a focal neurological deficit.

Adult↗
PubMed1980

[Surgical treatment of gastric cancer. New experimental reconstructive technic after total gastrectomy].

The Authors deal the always present problem of a proper reconstruction of the digestive tract after total gastrectomy operations for gastric cancer. The Authors, then, describe a experimental operation, after having dealth with the considerations concerning the symptomatic consequences that usual different kind of operations cause upon patients. That operation has been experimented upon animals, and consists in a neo-stomach. In this reconstruction they interpose a tract of jejunum loop between oesophagus and duodenum. The jejunum loop must be lengthwise dissected, twisted and sutured in such away as to obtain a new structure that will be last anatomically similar to a stomach. The Authors hope that this new technique might be successfully applied upon man sometime in the future.

Animals↗
PubMed1978

R factors from Enterobacter group.

Twenty-six R factors transmissible to E. coli K12 were derived from 79 strains of Enterobacter species isolated in various clinical specimens (urine, sputum, blood, pus); 71 of 79 strains were Enterobacter cloacae. Fifteen R factors were fi+: 14 belonged to group FII, 1 to group FI. Eleven R factors were fi-: 5 belonged to group M, of the others 6 (all controlling the cloramphenicol-resistance) 1 was recognized of group K, 1 was incompatible with standard plasmids of both groups S and W, and 4 belonged to group S.

Anti-Bacterial Agents↗