Search PubMed⌕ Search

Biomedical subjects

L P Smirnov

Publications and source records attributed to L P Smirnov.

10 recordsLinked to original sources
PubMedDate not supplied

[Cytoplasmic protein vaccine against bacterial hemorrhagic septicemia (aeromonosis) of fish].

Water-soluble proteins from Aeromonas sobria, a causative agent of bacterial hemorrhagic septicemia of fishes, were separated into six fractions by gel chromatography on Sephadex G-100. Injections of fraction II (67 kDa) provided the highest protection of carps against the disease. Injections of proteins contained in fraction II caused stronger effects on certain biochemical parameters in the fish liver (fatty acids of phospholipids and cathepsin B and D activities) in comparison to infections of the live culture.

Aeromonas↗
PubMedDate not supplied

[Lipids of microorganisms from the family Vibrionaceae causing diseases in fishes].

Lipid fractional and fatty acid compositions of microorganisms from the genera Aeromonas, Pseudomonas, and Vibrio (the family Vibrionaceae), causing diseases of different fish species, were studied. Motile aeromonads and vibrios displayed higher relative contents of membrane lipids and oleic acid and lower relative contents of storage lipids compared with immotile aeromonads and pseudomonads, which is connected with the activities of their movements. Immotile aeromonads and vibrios exhibited higher levels of polyunsaturated fatty acids and higher absolute phospholipid contents compared to motile aeromonads and pseudomonads. This is likely to be related to host specificity of these bacteria and reflects the specific patterns of fatty acid compositions of the infected fish (salmonid and cyprinid) tissues.

Animals↗
PubMedDate not supplied

[Biochemical characterization of aeromonad strains differing in pathogenicity].

A virulent strain of motile aeromonad (77-18) and an avirulent strain (78-16) differed in the contents of lipids and phospholipids, odd fatty acids, activity of hydrolytic enzymes, and amount of proteins with molecular weights of 47-56 kDa. It is assumed that proteins with molecular weights of 47-56 kDa, proteolytic enzymes active within a wide pH range, and odd fatty acids may act as pathogenicity factors. Each of these compounds or their combination determines certain stage of infection.

Aeromonas↗
PubMedDate not supplied

[Cestode lysosomes].

By differential centrifugation method a lysosomal fraction was obtained from five species of cestodes, which possesses the highest specific activity of acidic phosphatases as compared to other subcellular fractions. By isopyknic centrifugation in the density gradient of saccharose the lysosomal fraction is divided into primary and secondary lysosomes. Lysosomes of cestodes are similar to those of vertebrate animals in the character of fractional distribution of acidic phosphatase, sedimentation abilities and sensitivity of membranes to triton X-100.

Acid Phosphatase↗
PubMedDate not supplied

[Fatty acid makeup of the cestodes, Eubothrium crassum and Diphyllobothrium dendriticum].

The fat-acidic composition of E. crassum and D. dendriticum was investigated. Lipids of E. crassum differ in greater unsaturation as compared to these of D. dendriticum as well as in greater amount of acids of type omega 3, whereas acids of type omega 6 and stearic acid were found in extracts of E. crassum in less quantity than in D. dendriticum. This phenomenon is characteristic of organisms living under conditions of low temperatures.

Animals↗
PubMedDate not supplied

[Fatty acid composition of the helminths of cold- and warm-blooded vertebrates].

Studies have been made on fatty acid composition of 5 species of parasitic helminthes from fish, birds and mammals. As compared to the lipids from helminthes of endothermic animals (Diphylobothrium dendriticum and Toxascaris leonina), those from fish parasites (Eubothrium crassum, Triaenophorus nodulosus and Metechynorhynchus salmonis) are less saturated and contain higher amounts of penta- and hexaenic acids, acids of omega 3 type, being less rich in the content of stearic, oleic, dienic and omega 6 acids. It is concluded that the formation of fatty acid composition in helminthes is controlled not only by the feeding pattern of the host, but also by the temperature of the latter.

Animals↗
PubMedDate not supplied

[The effect of host temperature on the fatty acid composition of the total and membrane lipids in the cestodes Eubothrium crassum and Diphyllobothrium dendriticum].

A comparative qualitative and quantitative analysis of the fatty acid composition of total and lysosome membrane lipids of two cestode species Eubothrium crassum (from cold-blooded host) and Diphyllobothrium dendriticum (from warm-blooded host) has been carried out. It is shown that quantitative ratio of the fatty acids in the total and membranous lipids of the helminth of cold-blooded host was very similar whereas for the helminth of warm-blooded host fatty acid composition of membranous lipids was considerably more saturated compared with the one of total lipids. It is assumed that the high level of unsaturation of total lipids from D. dendriticum is associated with preadaptation mechanism of fatty acid spectra in parasites with the development coupled with a consecutive rapid change of different thermal regime habitats.

Animals↗
PubMedDate not supplied

[Activity of lysosomal proteinases in carb tissue in aeromonas infection].

The activity of major lysosomal proteinases (cathepsins B and D) increased in carp tissues infested with Aeromonas hydrophila. The value and character of changes in the activity of cathepsins depended on the degree of infestation and the physiological state of fishes (normally fed or starved).

Aeromonas↗