Biomedical subjects

L Naess

Publications and source records attributed to L Naess.

3 recordsLinked to original sources

PubMed1991

Toxic oxygen metabolites increase microvascular permeability in isolated perfused rat lungs: the effect of methylprednisolone.

Toxic oxygen metabolites (TOM) have been suggested to be mediators of permeability edema associated with the adult respiratory distress syndrome (ARDS). Because corticosteroids have possible beneficial effects in ARDS, we have examined the effect of methylprednisolone (MP) on TOM-induced lung edema in isolated, plasma-perfused rat lungs. TOM were generated by adding xanthine oxidase (XO) and hypoxanthine (HX) to the perfusate. Microvascular permeability was assessed by fluid filtration rate (FFR). FFR was determined before and 30 min after administration of XO and HX by measuring the weight increase of the lungs for the last 3 min during a standard 5 min elevation of the outlet pressure. MP was administered in two different ways: 1) Added to the perfusate 5 or 60 min before XO and HX (0.1 and 1 mg ml-1), and 2) given as pretreatment to the rats 12 and 2 hr before preparation of the lungs (40 mg kg -1). XO and HX significantly increased FFR compared to lungs perfused with untreated plasma. Pretreatment with MP significantly attenuated the increase in FFR caused by XO and HX. Addition of MP to the perfusate also inhibited the effect of TOM. This latter protection occurred irrespective of when MP was added before XO and HX. However, when the highest dose of MP was added 5 min before XO and HX, there was a loss of the protective effect. In summary, this study provides evidence that MP may directly prevent microvascular injury induced by TOM in isolated perfused rat lungs. The effect was dependent on the dose of MP applied, but not on when MP was administered prior to exposure to TOM.

Animals↗

PubMed1990

[Jet ventilation].

High frequency jet ventilation gives adequate alveolar ventilation with very small tidal volumes, gas exchange at lower airway pressure, reduced risk of pulmonary barotrauma, and less cardiovascular impairment. Use of small insufflation catheters facilitates surgery when access is restricted, as in laryngeal surgery, and makes it possible to ventilate a patient through a cricithyroid membrane puncture in an emergency situation. In intensive care medicine, jet ventilation can have some advantage over conventional ventilation in patients with bronchopleural fistulas, and when it is difficult to wean patients from a respirator. The article describes experience using an Acutronic AMS-1000 Universal Jet Ventilator.

Adult↗

PubMed1986

Uptake, intracellular transport and release of 125I-poly(vinylpyrrolidone) and [14C]-sucrose-asialofetuin in rat liver parenchymal cells. Effects of ammonia on the intracellular transport.

We have studied the intracellular transport of 125I-labeled poly(vinylpyrrolidone) (125I-PVP) and [14C]sucrose-asialofetuin (14C-SAF) in isolated rat hepatocytes. 125I-PVP and 14C-SAF are taken up in the cells by fluid phase and receptor-mediated endocytosis, respectively. The labeled degradation products formed from 14C-SAF are trapped in the lysosomes. They can therefore serve as markers for lysosomes in subcellular fractionation studies. The accumulation of 125I-PVP in the cells was rapid initially and then decreased to a constant value. The diminished rate of accumulation was due to release (exocytosis) of previously endocytosed 125I-PVP. The release of 125I-PVP was studied in cells that had accumulated 125I-PVP for various times and then after washing incubated in new medium at 37 degrees. About 25% of the radioactivity associated with the cells after 1 hr was released to the medium subsequently. No such release was observed in cells that had taken up 14C-SAF. Subcellular distribution of 125I-PVP and 14C-SAF was studied by isopycnic centrifugation in sucrose gradients. Both compounds were sequentially associated with light (1.13 g/ml) and dense (1.19 g/ml) vesicles. Exocytosed 125I-PVP was derived from the light vesicles. The denser organelles were probably lysosomes as their distribution coincided with that of lysosomal enzymes. By measuring radioactivity soluble and precipitable in trichloroacetic acid it could be shown that only degraded 14C-SAF was associated with lysosomes. Undegraded 14C-SAF was associated with vesicles banding at 1.13 g/ml. Degraded 14C-SAF was, however, also seen first in this region of the gradient, suggesting that degradation started in a light lysosome. Both uptake and release of 125I-PVP were temperature dependent; both processes ceased at 10 degrees. Ammonium ions had negligible effects on uptake and release of 125I-PVP. The amine inhibited, however, the transfer of both 125I-PVP and 14C-SAF to the lysosomes.

Acetylglucosaminidase↗