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Biomedical subjects

L Mendoza

Publications and source records attributed to L Mendoza.

At least 91 records · Page 5Linked to original sources
PubMed1990

Immunodiffusion test for serodiagnosing subcutaneous zygomycosis.

Culture filtrate antigens of Basidiobolus ranarum and Conidiobolus coronatus were analyzed by immunodiffusion (ID) with homologous rabbit antisera. B. ranarum and C. coronatus were each found to have five specific antigens. Results of tests with heterologous antisera indicated that all of the species shared at least one antigen. ID tests incorporating the specific precipitin bands as references were developed for detection of basidiobolomycosis and conidiobolomycosis. These tests were performed with sera from humans and horses with proven basidiobolomycosis and conidiobolomycosis as well as with control sera from humans and animals with and without heterologous mycotic and oomycotic infections. Only sera from cases of basidiobolomycosis and conidiobolomycosis produced lines of identity with the reference precipitates of B. ranarum and C. coronatus, respectively. The ID tests were found to be completely sensitive and specific for determining the etiology of zygomycosis caused by these two species. In addition they appeared useful for monitoring resolution of the infections.

Animals↗
PubMed1990

[Prevalence of hepatitis B surface antigen in general population of coast, mountain and forest regions of Peru. A preliminary report].

A prospective study was performed in order to establish HBsAg prevalence on population living in urban areas of three geographical regions of Peru. There were included 100 apparently healthy people of each city, with a residence major to 5 years, no migrate neither transitory. Each subdivided group in 50 adults and 50 children. Of these last ones 15 between 1 to 7 years old and 20 cases between 7 to 14 years old. Samples were obtained by venipuncture, then they were centrifuged and the serum obtained was stored at -20 degrees C before being sent to Lima for processing. Determination of HBsAg was made by the Elisa's technic (Abbott Lab-III.-USA). In this preliminary report we informed about results in 7 cities (Lima, Iquitos, Chiclayo, Arequipa,Ica, Chachapoyas y Tarapoto) that include 680 persons of which 373 were adults and 307 children. The HBsAg, was positive in 26 cases (3.8%). In the adult group were 13 positives (3.4%), and in the children also 13 cases (4.2%). Result of all sample shows figures of prevalence (3.8%) major to that reported before at national level. The same phenomenon was observed in the cost while in the jungle our results were similar to those found before. In the andean area not studied previously there were obtained similar results to the rest of the country. It is also interesting to mention the high percentages of prevalence in children. These results indicate that hepatitis B is a serious problem of public health in our country that merit to take immediate prophylactic measures.

Adult↗
PubMed1989

Antigenic relationship between Pythium insidiosum de Cock et al. 1987 and its synonym Pythium destruens Shipton 1987.

Antigens and rabbit-antisera from holotypes of Pythium insidiosum and P. destruens were prepared to elucidate their antigenic relationship. The antigens and rabbit-antisera of P. insidiosum as well as P. destruens used as a reference system showed that both shared three precipitin bands in common. The antigen and rabbit-antisera of P. destruens and P. insidiosum used as a reference system against other strains isolated from humans and animals with pythiosis, also showed three precipitin bands in common. When we used sera taken from horses with proven pythiosis against antigens of P. insidiosum and P. destruens, six common bands were observed. We concluded that the etiologic agent of pythiosis is a single species P. insidiosum, and could be identified by serologic methods.

Animals↗
PubMed1988

A method to obtain rapid zoosporogenesis of Pythium insidiosum.

Nine strains of Pythium insidiosum the etiologic agent of pythiosis, were inoculated on 2% water agar plus grass blades and then incubated one day at 25 degrees C, 35 degrees C and 37 degrees C. Sporangium and secondary biflagellate-type zoosporas from the parasitized grass blades were noticed in induction medium after one hour of incubation at 35 degrees C and 37 degrees C. The number of sporangia and zoospores were lower at 25 degrees C, than 35 degrees C and 37 degrees C. Increasing the days of incubation of the parasitized grass blades resulted in the increase in the time of incubation in the induction medium. Corn meal agar, Schmitthenner medium and Sabouraud dextrose agar were also tested but the sporangium and zoosporas were always observed after five hours of incubation in induction medium.

Animals↗
PubMed1988

Bone lesions caused by Pythium insidiosum in a horse.

A 5-year-old, female saddle horse developed a tumoral mass anterolaterally on the metacarpal region of its right front leg. Histopathological study showed hyaline, aseptate, broad hyphae in the sequestered coral-like necrotic masses. Radiographs revealed an extensive osteomyelitis with disorganized bone proliferation of the metacarpal bones, as well as exostosis of the distal radius and the proximal and distal row of carpal bones. The etiologic agent was detected histologically, isolated in culture and identified as Pythium insidiosum. Serological tests were positive. Immunotherapy was applied but no cure resulted. The horse was sacrificed and necropsy confirmed the X-ray findings.

Animals↗
PubMed1987

Idiopathic multicentric osteolysis with facial anomalies and nephropathy.

Idiopathic osteolysis denotes a group of rare bone disorders differentiated on the basis of clinical, radiological, and genetic criteria. Idiopathic multicentric osteolysis (IMO) is one form of osteolysis that can occur as an autosomal dominant condition. In childhood, affected individuals have arthritic-like episodes, followed by progressive deformities, radiological osteolytic changes, and variable degrees of disability. A peculiar face and variable renal involvement have been described as associated manifestations. We report on a family with three members affected by IMO in two generations showing variable bone changes and persistent proteinuria. A sporadic case with severe renal damage is also described in which a de novo dominant mutation is suggested. All patients had peculiar facial manifestations including triangular shape, protruding eyes, and micrognathia. These manifestations may be part of the syndrome of IMO.

Arthritis↗
PubMed1987

Antigenic relationship between the animal and human pathogen Pythium insidiosum and nonpathogenic Pythium species.

Identification of the newly named pathogenic oomycete Pythium insidiosum and its differentiation from other Pythium species by morphologic criteria alone can be difficult and time-consuming. Antigenic analysis by fluorescent-antibody and immunodiffusion precipitin techniques demonstrated that the P. insidiosum isolates that cause pythiosis in dogs, horses, and humans are identical and that they were distinguishable from other Pythium species by these means. The immunologic data agreed with the morphologic data. This indicated that the animal and human isolates belonged to a single species, P. insidiosum. Fluorescent-antibody and immunodiffusion reagents were developed for the specific identification of P. insidiosum.

Animals↗
PubMed1986

Equine pythiosis in Costa Rica: report of 39 cases.

Thirty-nine pythiosis equine cases, were studied at the Veterinary Medicine School of the National University of Costa Rica, between 1981 and 1984. Lesions were located in different parts of their anatomy: anterior and posterior extremities, abdomen, thorax, breast and mammary gland, and were characterized by their tumoral appearance with necrotic tissue in which yellow-white coral-like necrotic masses, called kunker or leeches were shown. Splendore-Hoeppli like phenomenon and eosinophilic inflammatory reaction around the hyphae, was microscopically observed. Pythium sp. (Hyphomyces destruens) was isolated in Sabouraud dextrose agar from ground kunkers. Immunodiffusion (ID) to diagnose this disease in equines, was performed with success. Immunotherapy was applied to 5 of affected horses, and three were cured. Some epizootiological aspects of the pythiosis are also discussed.

Animals↗
PubMed1986

Immunodiffusion test for diagnosing and monitoring pythiosis in horses.

A practical, sensitive, and specific immunodiffusion test was developed for diagnosing and monitoring pythiosis in horses. Culture filtrates, a soluble cell mass, and trypsinized Pythium sp. antigens were evaluated against prepared rabbit anti-Pythium sp. serum and pythiosis horse case sera. The culture filtrate antigens demonstrated the greatest capacity for detecting precipitins and the greatest stability during storage. In contrast, the trypsinized antigens had the weakest capability for detecting multiple precipitins and the poorest stability. The 13 sera from horses with proven active pythiosis were positive in immunodiffusion tests with the culture filtrate antigens. Each serum contained from three to six precipitins. Treated horses lost precipitins, and some became antibody negative. No false-positive reactions were noted in tests with sera from normal horses and humans or with sera from a variety of heterologous horse and human infections.

Animals↗
PubMed1985

Experiences in the use of eight cutaneous and osteocutaneous superficial and deep circumflex iliac free flaps.

Eight patients underwent free tissue transfers involving groin cutaneous or osteocutaneous flaps using the superficial and deep circumflex iliac arteries and veins. Four were used for head and neck reconstruction, two for severe neck scar contractures, and two for composite skin and mandibular reconstruction. Three were used in lower extremity reconstructions, all in situations requiring bone and skin reconstruction. One was used for skin cover alone in an upper extremity reconstruction for wrist cover. One of the head and neck cases sustained vascular thrombosis requiring a second successful free flap coverage for a 12.5% failure rate. One required re-exploration with successful salvage. The average operating time was 12 hours. The anatomy and surgical techniques are discussed. The main values of the procedure are the presence of a double vascular supply of both arteries and veins for complex reconstructions, and the fact that a large area of skin and subcutaneous tissue can be procured, with a relatively inconspicuous donor site.

Adolescent↗
PubMed1985

Canine mycotic keratoconjuntivitis caused by Acremonium kiliense.

Mycotic keratoconjuntivitis caused by Acremonium kiliense in a German Shepherd bitch was diagnosed with the aid of laboratory tests. The dog presented with photophobia, tearing, corneal edema and reduction of the visual capacity. A thick white layer partially covered the right eye. The left eye showed irritation and small brown stains which were diagnosed as pigmentary keratitis. The initial treatment consisted of 2% yellow mercury oxide. Natamycin was used as final treatment. Seven days later, the natural brightness of the eye as well as the visual capacity were restored.

Acremonium↗
PubMed1982

A demonstration that 5-hydroxytryptamine administered peripherally can affect sexual behavior in male rats.

In the male rat, subcutaneous injections for 7 days of 20 mg/Kg B.W./day of 5-hydroxytryptamine creatinin sulphate (5-HT), caused remarkable inhibitory effects on sexual behavior. The mount and intromission latencies were increased in rats treated with 5-HT, whereas ejaculation latency in the few rats treated with 5-HT that it achieved, was similar to that obtained in control rats. The mount and intromission frequencies were decreased in the rats treated with 5-HT. The mean inter-intromission interval (MIII) and post-ejaculatory interval were prolonged in rats treated with 5-HT. These data provide evidence for the role of peripheral 5-HT in regulating sexual behavior of male rats.

Animals↗