Search PubMed⌕ Search

Biomedical subjects

L Mastrolia

Publications and source records attributed to L Mastrolia.

32 records · Page 2Linked to original sources
PubMed1981

Comparative acid phosphatase distribution in the suprarenal gland of Discoglossus pictus, Xenopus laevis and Bufo bufo (Anurans, Amphibia).

Acid phosphatase activity was found to have a similar distribution in the suprarenal glands of Discoglossus pictus, Xenopus laevis and Bufo Bufo (Anurans, Amphibia) as determined by light and electron histochemical localization. The enzymatic activity is localized in the lysosomes of both the interrenal cells and the chromaffin cells. It is, moreover, positive on the granule membranes of the adrenaline cells whereas it appears only occasionally on the granule membranes of the noradrenaline cells. Some precipitates can also be seen occasionally at the level of the Golgi membranes.

Acid Phosphatase↗
PubMed1979

[Some observations on the formation of the primary tunica albuginea in the ovary of the chick embryo (author's transl)].

The ovarian cortex of chicken embryos of 10 to 14 days of incubation was observed in order to study the formation of the primary tunica albuginea and to discern in its cells an ultrastructural confirmation of an eventual steroidogenic capacity which has been proposed by some authors. From observations at the electron microscope, it is probable that the mesenchyme-like cells which constitute the primary tunica albuginea may originate from the somatic cells of the cortex, either from those of the germinal epithelium, or from those which, because of their position, may be termed prefollicular. There is not apparent ultrastructural confirmation of an active steroidogenic capacity in the albugineal cells. A possible physiological difference between clear cells and dark cells due to a different electron density, is furthermore discussed.

Animals↗
PubMedDate not supplied

Ontogeny of the thymus in a teleost fish, Cyprinus carpio L.: developing thymocytes in the epithelial microenvironment.

A monoclonal antibody, WCL9, specific for membrane molecules of a thymocyte subpopulation was used to detect these cells in situ during the ontogeny of thymus. Cryo-sections revealed WCL9+ cells in the rudiment of the thymus (day 4 post fertilization); thereafter, the positive cells were observed exclusively in the cortex from the first appearance of thymic regionalization (week 4 post fertilization) until adult age. Whole-mount immunostaining of the thymus with WCL9 revealed the three-dimensional structure of the cortex by specific staining. The presence and distribution of apoptotic cells during thymus development was studied by in situ end-labelling of fragmented DNA. From week 4 post fertilization onwards, apoptotic cells were more frequently detected in the cortex than medulla, suggesting a continuous selection of thymocytes in the cortex. Ultrastructural studies confirmed the presence of numerous cortical apoptotic cells inside macrophages. Electron microscopy provided evidence for the existence of epithelial heterogeneity in the thymus. During the ontogeny, the differentiation of epithelial cells was followed from the first weeks until the juvenile age. Cell types were classified on the basis of their localization and cytological characteristics as: i) limiting epithelial cells located in subcapsular, perivascular and peritrabecular zones; ii) reticular epithelial cells situated in medullary and cortical zones; iii) nurse-like cells at the border between the cortex and medulla, iiii) Hassall's body-like structures localized in the medulla. This study could suggest the occurrence of a wide range of lympho-epithelial interactions throughout thymocytes differentiation.

Animals↗