[Technical difficulties in clinical immunohaematology].
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ABO Blood-Group System↗
Biomedical subjects
L Mannessier
Publications and source records attributed to L Mannessier.
[Follow-up of the feto-maternal allo-immunization].
Immunohaematological tests used in antenatal patients have come a long way. However, despite a great deal of progress, we should not loose sight of the fact that these tests give only an indirect measurement and will only help the obstetrician, in conjunction with other fetal parameters, to assess the severity of the haemolytic disease (HD) of the fetus and newborn. The best method to assess the severity is the direct determination of foetal blood group hemoglobin after foetal blood sampling but this procedure is not without risk. Since 10 years ago, it is possible to determine the RHD genotype of the fetus using amniocytes and, today, maternal plasma directly. All pregnant women should be grouped for ABO-RH-KEL1 and the sera tested for clinically irregular antibodies (anti-RH are still the most frequent). The trend in anti-RH levels is more important than the level itself. The perfect technique for anti-RH quantitation has not been developed. Manual titration is simple but only provides rough, semiquantitatives estimates of anti-RH concentration. Quantitative haemagglutination methods, using auto-analyzers and appropriate anti-RH1 standards, measure in microg/ml, are sensitive, rapid and have acceptable intra-laboratory reproductibility.
Erythroblastosis, Fetal↗
[Critical analysis of the new ministerial order about guidelines in immunohaematology].
The evolution of the methods used in immunohematology is a constant concern for all those involved in the process. Thus, during the last few years, new technologies have been introduced which aim at improving performance. This improvement is not limited to the search for an overall increase in specificity-sensibility; it also takes into account the capability to detect "the clinical significant" as well as the limitations of human reliability, which justifies the introduction of automation and computerization. The whole of these methodological evolutions associated with that of the performance of re-agents, legitimate to modify the law about the realization of erythrocyte typing and detection of irregular antibodies. In order to ensure the immunohematologic safety of transfusions, it was also necessary to place the entire transfusion process under computer control: patient identification, sample collection, test results and release of blood.
ABO Blood-Group System↗
[External quality evaluation].
Since ten years, the immunohaematology working group of the French Society of Blood Transfusion has organized a quality control. Tests concern essentially the screening and identification of irregular antibodies, direct antiglobulin tests and elutions.
Autoantibodies↗
[Problem-solving in immunohematology: direct compatibility laboratory test ].
Cross-matching between the serum of a patient and the red blood cells to be transfused is most important for the prevention of hemolytic transfusion reactions in allo-immunized or new-born patients found positive with direct antiglobulin test. Cross-matching is a time-consuming and complex laboratory test. In order to obtain valid results, it is necessary to abide by some technical rules detailed in this article. The choice of the blood units to be cross-matched depends on the patient's clinical story and on the specificity of anti-erythrocyte antibodies present in the serum. The identification and the management of most frequent difficulties met by using the cross-match technique are discussed hereby.
Adult↗
[Evolving methods in immunohematology].
The immunogenic nature of erythrocyte polymorphism is in variance with the incompatible transfusion. Indeed, the fixing of an antibody on the corresponding antigen generally condemns the cell concerned with its destruction. Therefore, in order to ensure the immunohemolytic safety of the transfusions, it is necessary to avoid an in vivo encounter between antigens and antibodies, whose feasibility study in vitro is a determining element. Because of the requirement standards of such analyses and the preoccupation with the continuous improvement of transfusion safety, the evolution of the methods used in immunohematology is a constant concern for all those involved in the process. Thus, during the last few years, new technologies have been introduced which aim at improving performance and sometimes implementing alternatives to agglutination. This improvement is not limited to the search for an overall increase in specificity-sensitivity; it also takes into account the capability to detect "the clinically significant" as well as the limitations of human reliability, which justifies the introduction of automation and computerization. The whole of these methodological evolutions associated with that of the performance of reagents, legitimate the need to reconsider the realization of erythrocyte typing and the search for anti-erythrocyte antibodies.
Agglutination Tests↗
[Analytical validation in erythrocyte immunohematology].
In a transfusional or foeto-maternal context, hemolysis by incompatibility due to anti-erythrocyte antibodies (regular or irregular) remains the most frequent and most serious immunological risk in the receiver. In order to prevent this risk, a number of actions must be taken, such as the realization of the immunohematologic analyses for which the methodological practices have been legislated because of their serious clinical consequences. Several elements play a role in the reliability of the analyses and their results: the selection of the reagents and their validation in the routine technique used; the validation of reception; the controls involved in secondary preparations (e.g., blood cells reagent); and the daily internal controls. All this requires the choice of adapted controls and the management of possible anomalies.
Adult↗
Decision aides in immunohematology: detection of anti-erythrocyte antibodies.
Detection and identification of irregular red-cell antibody in the serum or plasma of a patient is of prime importance for the prevention of hemolytic transfusion reactions and the biological supervision of the hemolytic disease of the foetus or the newborn. Practice in these tests is replete with complex biological problems. Using problem solving strategies, we discuss the recognition and resolution of the most frequent difficulties encountered in red cell antibody identification.
Algorithms↗
[Problem-solving in immunohematology: interpretation of ABO typing and its difficulties].
Practice in immunohematology is replete with complex problems that require practitioners' problem-solving performance. In immunohematology, the acquisition of the reasoning process and necessary skills for making clinical decisions is based on teaching problem-solving strategies which potentially reduce errors and improve patient outcome. We discuss the recognition and resolution of the common causes of discrepancies in ABO typing results using problem-solving strategies.
ABO Blood-Group System↗
[Prevention of fetomaternal alloimmunization].
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Erythroblastosis, Fetal↗
[Prevention of fetal hemolytic disease: it is time to take action].
In spite of the progress made since 1970 in specific prevention by anti-rhesus immunoglobulins, and improved management of at-risk pregnancies, allo-immunization due to the erythrocytic Rh 1 antigen (formerly known as Rhesus D or Rh D) remains widespread. In fact, anti-Rh 1 antibodies currently constitute over one-third of the immune antibodies detected after pregnancy. The prevention of allo-immunization against the Rh 1 antigen is therefore still problematical, and concerns approximately one pregnant woman in seven. The etiology and pathology of fetal hemolytic disease have been recalled, and the treatment approach during pregnancy and delivery has been carefully examined. Tests for quantifying the risk of fetomaternal hemorrhage have also been described. This approach aims at improving the methods of preventing allo-immunization (e.g., during pregnancy and delivery) and the efficacy of treatment. It is also stated that if the necessary preventive action is not taken in cases of allo-immunization due to to the Rh 1 antigen, this should be considered a grave medical fault.
Erythroblastosis, Fetal↗
[Prevention of hemolytic disease of the fetus and the newborn: it is necessary to act].
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Blood Grouping and Crossmatching↗
[Detection of irregular anti-erythrocyte antibodies using the indirect antiglobulin test in a low-ionic-strength medium. Immunohematology Group of the French Blood Transfusion Society].
The detection of irregular antibodies is usually performed with serum by the indirect antiglobulin test (IAT) with a polyspecific antiglobulin reagent. In a first study in 1996, we compared the results obtained with 3,264 blood samples of patients drawn with or without anticoagulant: no significant difference was observed among the 240 allo-antibodies detected and identified. In this paper we report the comparison of the results obtained by IAT on column of filtration with two kinds of reagents: polyspecific and anti-IgG antibodies. Respectively 2,927 (76 contained an antibody), and 643 (161 contained an antibody) sera of patients were tested with methods ID-Diamed and Ortho-Biovue. Titrations of 153 other antibodies were also performed with the two reagents. Results showed no significant difference using either polyspecific reagent or the anti-IgG antibodies. This study proves that it is possible to perform screening of irregular antibodies on uncoagulated blood samples. This possibility allows automation as blood typing and screening of irregular antibodies can be carried out with the same sample.
Blood Group Antigens↗
[Identification of alloantibodies associated with an autoantibody: profile of 2 quality control programs in French blood transfusion facilities].
The French Blood Transfusion Society working group Immunohaematology and the French Blood Transfusion Center of Lille performed two quality control exercises-96-01 and 97-02-in order to evaluate identification performances of alloantibodies associated with an autoantibody recognizing a high frequency antigen. Concerning control 96-01, 83 (75%) of the 110 blood transfusion centers participating at this exercise sent results. The alloantibody screening was correct for 78 of them (94%). Sixty-one (78%) blood transfusion centers correctly identified the specificities (anti-RH1 + anti-FY1). Concerning control 97-02, 82 (94%) of the 87 blood transfusion centers participating in this exercise sent results. The alloantibody screening was correct for 69 (88%) of them. Fifty-three blood transfusion centers (77%) correctly identified the specificities (anti-RH3 + anti-FY1). These exercises allowed us to confirm the main procedures used in routine for national scale testing. The analysis of the results has underlined the importance of these tests for assessing the quality of these examinations, and highlighted the means to be carried out in order to improve them.
Antibodies, Anti-Idiotypic↗
[Fetal cells in the maternal blood: a step towards non-invasive prenatal diagnosis? Review of the literature].
Prenatal diagnosis of genetic abnormalities requires nucleated fetal cells which are currently obtained by invasive techniques such as amniocentesis, chorionic villus sampling and percutaneous umbilical blood sampling. Each of these entails a risk to the foetus and sometimes to the mother. Nucleated fetal cells have been reported to be present in maternal blood. Recovery of fetal cells from maternal blood would allow a noninvasive prenatal diagnosis. Their rarity (1 fetal cell for 10(6) to 10(8) maternal cells) presents a technical challenge. Due to the small number of fetal cells, sensitive analysis techniques such as PCR and FISH are necessary. Some degree of fetal cells enrichment in the maternal blood sample often precedes the analysis. Different techniques are used for the enrichment: discontinuous density gradient, magnetic activated cell sorting, fluorescence activated cell sorting, micromanipulator.... Several prenatal diagnosis have already been performed from maternal venous blood samples: diagnosis of gender, RhD blood genotype, Duchenne muscular dystrophy and hemoglobinopathy by PCR, diagnosis of gender and chromosome aneuploidy by FISH. Many teams are working on this subject. It is difficult to compare the studies because the techniques of enrichment and analysis vary. We review the different strategies chosen for prenatal diagnosis from maternal blood and discuss the results.
Cell Nucleus↗
Thrombocytopenia induced by vancomycin-dependent platelet antibody.
BACKGROUND AND OBJECTIVES: Many drugs are associated with thrombocytopenic purpura through immune-mediated platelet destruction. The case of a woman who suffered life-threatening thrombocytopenia during vancomycin treatment for Staphylococcus aureus septicemia is reported. MATERIALS AND METHODS: Conventional clinical and laboratory methods, including flow cytometry. RESULTS: After treatment of septicemia with vancomycin, severe thrombocytopenia and bleeding occurred, without detection of drug-dependent platelet antibodies (DDPA). This was followed by vegetative endocarditis, whereupon antibiotics were withdrawn so as to isolate the organism. The thrombocytopenia was corrected. On day 34, antibiotics including vancomycin were reinstituted, and three days later thrombocytopenia recurred. With a change in antibiotics, the platelet count corrected itself within four days. CONCLUSIONS: Vancomycin may induce potentially severe immunological thrombocytopenia.
Aged↗
[Prenatal diagnosis using fetal cells isolated from a maternal sample. Literature review].
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Erythroblasts↗
[The direct antiglobulin and elution tests: evaluation of quality control in Blood Transfusion Centers].
The Société Française de Transfusion Sanguine and the Centre National de Référence pour les Groupes Sanguins performed a quality control to evaluate the performances of two serological tests: the Direct Antiglobulin Test (DAT) and the Elution test. Among the 110 Blood Transfusion Centers participating in this control, 80 (73%) returned a result. Of these, 68 results were correct for the DAT (85%; positive for type IgG) and 31 results were correct for the elution (39%; anti-FY1). This control gave the opportunity to confirm the main procedures used in routine testing on a national scale. The analysis of the results underlines the importance of the choice of a standardized technique for these tests. Such controls are useful to appreciate the quality of the routine tests and to find the means to improve them.
Blood Transfusion↗