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Biomedical subjects

L M Watson

Publications and source records attributed to L M Watson.

5 recordsLinked to original sources
PubMed2005

Suppression of the cysteine protease, aleurain, delays floret senescence in Brassica oleracea.

An aleurain-like protein, BoCP5, is up-regulated during harvest-induced senescence in broccoli floret and leaf tissue. BoCP5 is most closely related to an Arabidopsis protein (91%, AAF43041) and has 71% identity to barley aleurain (P05167). The mRNA for this gene accumulates within 6 h after harvest in broccoli florets, and its expression is reduced in tissue that has been held in senescence-delaying treatments (e.g. water, sucrose feeding, controlled atmosphere). The gene is also expressed in leaves during aging-related and harvest-induced senescence. Analysis of protein bands that cross-react with antibodies raised to the bacterial BoCP5 fusion protein, revealed prominent immunoreactive bands at ca. 26, 28, 31, and 38 kD in floret tissue. The 31 kD band was absent in protein extracts from leaf tissue. Agrobacterium-mediated transformation was used to produce transgenic broccoli plants with down-regulated BoCP5. A reduction in the postharvest expression of BoCP5 in floret tissue was achieved for four transgenic lines in the current study. In three of these lines postharvest floret senescence (yellowing) was delayed, and florets contained significantly greater chlorophyll levels during postharvest storage at 20 degrees C than wild-type plants. Line 4 showed the greatest down-regulation of BoCP5, and in this line postharvest protease activity remained at pre-harvest levels, and the yield of soluble proteins extracted from florets after harvest was significantly greater than that of wild-type tissue.

Amino Acid Sequence↗
PubMed1996

Vehicle entrapment rescue and pre-hospital trauma care.

From 1 April 1991 to 31 March 1993 the Royal London Hospital Helicopter Emergency Medical Service (HEMS) attended 737 road traffic accidents, 90 (12 per cent) of which involved entrapments. Nine casualties (10 per cent) died, of which five died at the scene. Thirty-two patients with a median ISS 17 (range 1-59) were transported by helicopter to the Royal London Hospital. Of these, four subsequently died in hospital. The median ISS of the non-survivors was 44 (range 24-59). The remaining 53 patients were transported to the nearest hospital. In 45 cases (50 per cent) patient extrication took longer than 30 min. The methods and standards for the release of trapped road traffic accident victims were reviewed to allow physicians a working understanding of Fire Service techniques. Entrapment rescue should create rapid access to the accident victim, allowing stabilization and release with minimum delay. Immediate and uncontrolled release of trapped victims is only indicated if there is immediate danger to life from the surroundings. We recommend a rigorous target of less than 30 min for the release of the casualty. To achieve this will require systematic extrication training for Fire Service crews and medical teams who are involved in immediate care.

Accidents, Traffic↗
PubMed1982

Illness-specific cards--a feasibility study.

To reinforce and extend advice given at consultations, take-home cards for 12 conditions were devised. They were assessed by 32 general practitioners and 306 patients. The response on the whole was favourable and we suggest that this form of patient education could be helpful in primary care.

Family Practice↗