Proceedings: Mechanism of the interferon-induced block of mRNA translation in mouse L cells and its reversal by tRNA.
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Animals↗
Biomedical subjects
L M Shulman
Publications and source records attributed to L M Shulman.
Isolation and characterization of West Nile virus from the blood of viremic patients during the 2000 outbreak in Israel.
We report the isolation of West Nile (WN) virus from four patient serum samples submitted for diagnosis during an outbreak of WN fever in Israel in 2000. Sequencing and phylogenetic analysis revealed two lineages, one closely related to a 1999 New York isolate and the other to a 1999 Russian isolate.
Animals↗
The prescription for long-term care. Improving the care of chronic conditions will have the single greatest effect on the long-term care crisis.
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Aged↗
IL-6 receptors and sensitivity to growth inhibition by IL-6 in clones of human breast carcinoma cells.
Pure recombinant human IL-6 inhibits growth of T47D, MCF-7 and SK-BR-3 human breast carcinoma and OVCAR-3 ovarian carcinoma cells in cultures. Subcloning of the breast ductal carcinoma T47D cells yields clones with high and low sensitivity to the growth inhibitory effect of IL-6. The subclones vary 40 fold in their sensitivity for inhibition of colony formation in sparse cultures and 200 fold for inhibition of thymidine incorporation in subconfluent cultures. Binding studies with 125I-rIL6 show that T47D cells and their subclones, as well as SK-BR-3 and MCF-7 cells, express high-affinity receptors for IL-6. The number and affinity constant of these receptors are comparable to those on lymphocytic and myeloid cells, and show no correlation with the high or low sensitivity phenotype. Proliferation of the breast cancer cells is inhibited by IL-6 in a cell density dependent manner, and is not due to a cytotoxic effect. In addition, IL-6 induces a morphological change with loss of epithelial characteristics and of cell-cell adhesion. Sensitivity to growth inhibition by IL-6 is independent from that of IFN-beta 1, IFN-gamma or TNF.
Breast Neoplasms↗