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Biomedical subjects

L M Kelley

Publications and source records attributed to L M Kelley.

25 records · Page 2Linked to original sources
PubMed1975

Quantitative assay of dermatophyte-infected guinea pig skin scales.

A technique to obtain homogeneous suspensions of fungal particles in scrapings from infected skin was devised that permits delivering uniform inocula to culture media being evaluated for suitability as primary isolation media for dermatophytes. The technique consisted of trypsinization of the scales, utilizing scrapings from lesions experimentally induced with an isolate of Trichophyton mentagrophytes var. granulare on guinea pigs. Optimal conditions for dislodging the fungal particles were assessed by colony counts. One-hour treatments with 0.5% (1:300) trypsin yielded the highest counts.

Animals↗
PubMed1975

Pagano-Levin Candida test medium: evaluation using vaginal samples.

Since 1958 pagano-Levin medium (PLM) has been used as an aid in the identification of Candida albicans. However, no statistical analysis of its effectiveness based on large number of random human specimens has been reported. The present study compared PLM (now called Candida test) with Sabouraud's plus antimicrobials and Littman's ox-gall agar without antimicrobials. Of 500 random vaginal samples 24.8 were true positives, 71.9% were true negatives, 2.2% were false positives, and 1.2% were false negatives on PLM. If only samples identified as C. albicans were considered, 95.4% were true positives and 4.6% were false negatives. PLM did not inhibit C. albicans from the vagina. No one medium was found superior to the others for the purpose of isolating and identifying C. albicans. Of the five strains of C. parapsilosis, the only other Candida species isolated, all the samples grew on PLM but most were inhibited on Sabouraud's medium plus antimicrobials.

Candida↗
PubMed1974

Interaction of pH and NaCl on enumeration of heat-stressed Staphylococcus aureus.

The effect of pH level and NaCl concentrations, alone and in combination, on the enumeration of unstressed and heat-stressed cells of three strains of Staphylococcus aureus was determined. A definite narrowing of the optimum pH range for enumeration of both unstressed and heat-stressed cells was observed as the NaCl concentration was increased from 0.0 to 7.5%. Counts of unstressed cells diminished only slightly with increases in NaCl, whereas heat-stressed cells showed a marked sensitivity to NaCl concentrations of 4% and above, regardless of the pH level. Because of this sensitivity to NaCl, recoveries were far poorer than with unstressed cells at NaCl concentrations of 4% and above.

Analysis of Variance↗
PubMed1973

Macrophage migration technique using coccidioidin.

Guinea pigs sensitized by repeated skin testing with potent, merthiolated coccidioidin, equal to Smith standard, exhibited erythema, edema, and an induration-like reaction to intradermal injection of the preparation. No statistically significant migration inhibition of peritoneal exudate macrophages from these animals was observed.

Animals↗
PubMed1969

Stability of St. Louis encephalitis virus in the airborne state.

The aerosol stability of St. Louis encephalitis (SLE) virus was studied over a 6-hr period at a temperature of 21 C and relative humidity values of 23, 46, 60, and 80%. Aerosols were generated from and collected in 0.75% bovine albumin-buffered saline, and spores of Bacillus subtilis var. niger were used as the tracer to determine the physical decay of the aerosols. Aerosol samples were titrated in BHK-21 cell monolayers for surviving SLE virus. The results of this study indicated that, under the test conditions employed, relative humidity had no influence on the stability of SLE virus in the airborne state.

Aerosols↗