A test of radial hemolysis in gel (RHG) has been developed and first used for serodiagnosis of tick-borne encephalitis (TBE). In examinations of 464 blood serum specimens from 258 patients with TBE and subjects suspected of this disease in RHG and HI tests the diagnosis of tick-borne encephalitis was confirmed in 77 subjects, the results of both tests being in complete agreement. A comparative analysis of antibody levels by RHG and HI tests revealed a complete correlation. An advantage of RHG over HI test is its insensitivity to serum inhibitors and the possibility of quantitation of antibodies in whole sera which omits a complicated procedure of serum treatment to remove inhibitors and serum titrations in serial dilutions mandatory for HI tests. The specificity and sensitivity of RHG, simplicity in running and the possibility of analysing many sera within a short time recommend the RHG test for public health practice for TBE serodiagnosis.

Antibodies, Viral↗

PubMedDate not supplied

[Epidemic manifestation of natural foci and the tasks in preventing tick-borne encephalitis in the USSR].

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Disease Outbreaks↗

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[Problems in detection of mycoplasma contamination in cell cultures (author's transl)].

A total of 135 cell lines was examined for mycoplasma contamination using two techniques: isolation and specific DNA-staining with DAPI or "Hoechst 33258". The two techniques showed similar results in 64% of the cases of cell contamination while the remainder was detected only by one or the other techniques: 12,82% by the staining technique and 23% by the isolation technique, which shows that the 2 techniques are complementary. The staining technique is very quick, easy to execute, very sensitive, and should be the method of choice to detect contamination when the mycoplasma do not grow in acellular media.

Animals↗

PubMedDate not supplied

[Immunoenzyme method of determining human lactoferrin and its use for diagnosing purulent-septic complications].

A solid-phase enzyme immunoassay of human lactoferrin was developed, which was used in diagnosis of some septic diseases. The test system developed enabled lactoferrin to be measured in the blood serum and plasma of healthy volunteers: 955.8 +/- 74.4 and 405.5 +/- 27.7 mg/e, respectively. This assay may be used also in measurement of blood serum lactoferrin levels in various pathological states and particularly applied for detection of postnatal pyoseptic failures before their clinical manifestations.

Humans↗

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[Absorption of essential amino acids in patients with chronic enteritis].

Continuous perfusion was applied in studying the absorption of essential amino acids in the duodenum and the proximal part of the jejunum in healthy persons and in patients with chronic enteritis of the land ii degrees of severity. All the patients demonstrated a reduced rate of absorption. The degree of the absorption derangement increased parallel with the severity of the affection. In the duodenum the absorption proceeded slower than in the upper portion of the jejunum.

Amino Acids, Essential↗