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Biomedical subjects

L M Fink

Publications and source records attributed to L M Fink.

At least 55 records · Page 3Linked to original sources
PubMed1990

Synchronous diffuse well-differentiated lymphocytic lymphoma and gastric adenocarcinoma presenting as splenomegaly and iron deficiency anemia.

Diffuse well-differentiated lymphocytic lymphoma (D-WDLL) and chronic lymphocytic lymphoma (CLL) represent closely related neoplasms which may have indolent courses. Dating back more than one century, reports of associated second primary malignancies continue to intrigue clinicians. A case of synchronous D-WDLL and gastric adenocarcinoma, presenting as splenomegaly and iron deficiency anemia, is presented. The case and literature are reviewed.

Adenocarcinoma↗
PubMed1987

Collagen-binding of von Willebrand's factor antigen in the classification of von Willebrand's disease.

Analysis of the multimeric structure of von Willebrand's factor (vWf) was compared with vWf-binding to collagen in subjects' plasma. The percentage binding of vWf to collagen was determined by both an enzyme-linked immunosorbent assay (ELISA) method and quantitative immunoelectrophoresis. Normal subjects, type I von Willebrand's disease (vWd), and type II vWd were studied. The authors have found by correlating collagen-binding results with multimeric analysis that normal controls exhibit 85.4 +/- 5.1% collagen absorption of their vWf, patients with type I vWd 80.8 +/- 5.3%, whereas patients exhibiting a deficiency of high molecular weight vWf have 32.3 +/- 16.6% collagen absorption of vWf. The results suggest that this functional assay of vWf can be used in the classification of vWd and in the detection of new dysfunctional forms of vWf.

Antigens↗
PubMed1986

Acid and gastrin levels following pyloric-preserving pancreaticoduodenectomy.

Acid and gastrin production after pyloric-preserving pancreaticoduodenectomy was evaluated in six patients. Five patients had low-normal basal and stimulated acid output; the sixth patient was achlorhydric. Fasting gastrin levels were less than 90 to 105 pg/mL (normal range) in five patients, three of whom had stimulated gastrin levels that remained below this range. Two patients had stimulated gastrin levels of 510 pg/mL and 205 pg/mL, respectively, within 15 minutes of eating; however, both levels returned to normal by 120 minutes' time. The sixth patient had mildly elevated fasting (105 pg/mL) and stimulated gastrin levels (160 to 200 pg/mL) throughout the test period. The results suggest that pyloric-preserving pancreaticoduodenectomy does not lead to either gastric hyperacidity or persistent hypergastrinemia.

Achlorhydria↗
PubMed1984

Perfluorochemicals. Morphologic changes in infused liver, spleen, lung, and kidney of rabbits.

Perfluorochemicals have been shown to have a high affinity for oxygen and therefore, have potential use in circumstances in which conventional blood transfusions are not possible. We examined by light and electron microscopy (EM) histologic changes in liver, spleen, lung, and kidney of rabbits each infused with 20 to 40 mL of perfluorotributylamine or with lactated Ringer's solution (controls). Rabbits were killed at 1, 2, and 3 weeks after infusion. Spleen and liver showed the most marked alterations. Spleen architecture was distorted with focal infiltration by foamy macrophages. The EM showed macrophages containing small and large, membrane-bound vacuoles. In the liver, Kupffer's cells were enlarged with eccentric nuclei and vacuolated cytoplasm. Hepatocytes contained occasional perfluorochemical particles. In the lung, intimal cells were swollen in the muscular arteries. While in alveolar septae, perfluorochemical particles could be identified ultrastructurally in the alveolar phagocytes and in the endothelium of capillaries. Kidney changes were more subtle, with occasional foam cells seen within the mesangium. The number of vacuoles did not decrease with time in any of the organs examined. Therefore, perfluorotributylamine particles are retained in vital organs; what effects these particles may have on normal tissue function need further evaluation.

Animals↗
PubMed1982

An alteration in the phosphorylation of vimentin-type intermediate filaments is associated with mitosis in cultured mammalian cells.

Analysis of cultured Chinese hamster ovary (CHO) cells has shown that vimentin exists primarily as two 57,000 dalton isoelectric variants, a nonphosphorylated form and a slightly more acidic phosphorylated form. Similar analyses of CHO cells that were treated with colcemid show the presence of at least two to three additional, more acidic, phosphorylated vimentin isoelectric variants. An increasing 32P-specific activity of these variants suggests that this alteration involves increased phosphorylation. Analysis of 32P-labeled vimentin from colcemid-treated cells indicates that the amount of the additional phosphorylated variants correlates with the accumulation of cells in mitosis. CHO cells enriched in mitotic cells without antimitotic drugs demonstrate the same alteration in the isoelectric focusing pattern of phosphorylated vimentin. When mitotic cells are replated, the amount of additional phosphorylated variants is reduced within 30 min. The data suggest that an alteration in phosphorylated vimentin is temporally related to the alteration in the organization of intermediate filaments in mitotic cells.

Animals↗
PubMed1979

Analysis of transmembrane proteins from eukaryotic cells.

The topography and properties of plasma membrane proteins from mouse L-929 cells are studied by comparing their availability for enzymatic labeling on the external and internal surfaces of the membrane. In order to study the internal surface, phagolysosomes are prepared from cells after they ingest latex particles. The plasma membrane surrounding these seem to have an "inside-out" orientation. The sugars of the membrane glycoproteins in intact phagolysosomes are not available for interaction with lectins or available for periodate-borotritide labeling. A comparison of the lectin-binding proteins labeled by lactoperoxidase-catalyzed iodination on the external cell surface with those labeled on the internal cell surface suggests that a variety of plasma membrane glycoproteins span the lipid bilayer. Using two-dimensional gel electrophoresis it has been shown that selected proteins are labeled at both the internal and external faces of the plasma membrane. Analysis of the 2-D gel electrophoregrams reveals that there are two distinct prominent proteins at 60,000 and 100,000 daltons which are enzymatically iodinated from both sides of the membrane. The partial hydrolysis of the 100,000 dalton protein reveals that different peptides are iodinated when the iodination is performed on intact cells or on the phagolysosomes. These proteins are extensively phosphorylated in cells incubated with inorganic 32P. We conclude that the phagolysosome is probably oriented in an "inside-out" configuration and that this membrane preparation can be used to study the topographic organization of membrane proteins. The use of oriented membranes, selective labeling of proteins, and affinity separation of proteins in combination with gel electrophoresis to define the position and properties of proteins is discussed.

Animals↗
PubMed1979

Asymmetric distribution of plasma membrane proteins in mouse L-929 cells.

The distribution of plasma membrane-associated proteins was studied by using latex-filled phagolysosomes prepared from cultured mouse L-929 cells as a model of "inside-out" membrane. Proteins from 131I/lactoperoxidase-labeled phagolysosomes, phagolysosomes derived from 131I/lactoperoxidase-labeled cells, and phagolysosomes prepared from [35S]methionine metabolically labeled cells were analyzed by high-resolution two-dimensional gel electrophoresis. The gel patterns of iodinated proteins showed specific differences in the availability of membrane proteins to lactoperoxidase labeling between inside-out and right-side-out membranes. However, at least two prominent [35S]methionine-labeled proteins of approximately 60,000 and 100,000 daltons were available for iodination at both sides of the membrane. Partial proteolysis of the 100,000-dalton protein revealed that different peptides were iodinated when the iodination was performed on intact cells or on phagolysosomes, consisent with the idea that this protein spans the plasma membrane.

Animals↗
PubMed1978

In vitro plasminogen activator activity in human brain tumors.

Cell cultures were prepared from nine human brain tumors. Fibrin plate assays showed plasminogen-dependent fibrinolytic activity in lysates and in material released by these neoplastic cells but not in those from normal adult human white matter. Antibodies against human urokinase caused catalytic inhibition of the urokinase and of the plasminogen activator from WI-38 cells, simian virus 40-transformed WI-38 cells, human prostatic cells, and human ovarian carcinoma cells. However, the anti-urokinase immunoglobulin G did not inhibit the plasminogen activator activity of any of the human brain tumor preparations. These studies indicate that the plasminogen activator produced by human brain tumor cells is antigenically different from the plasminogen activator of other human normal and neoplastic cells.

Brain Chemistry↗