Biomedical subjects
Publications and source records attributed to L Luo.
This paper introduces the system construction of Transurethral Prostate Thermotherapy Device. The temperature measuring device, I/O interface circuit and the programming principle of PID controlling system is illustrated also.
OBJECTIVE: To determine whether fetal fibronectin is a sensitive test for the diagnosis and prediction of preterm birth. METHODS: Fetal fibronectin was measured by enzyme-labeled immunosorbent assay (ELISA) methods in sample of cervical secretion from 38 women with threatened preterm delivery (study group) and 43 normal pregnanty women with same gestational weeks (control group). RESULTS: The positive rate of fetal fibronectin in women with threatened preterm birth was significantly higher than that in the normal control group. CONCLUSION: Fetal fibronectin is an important marker. It can be used for diagnosis and prediction of preterm birth.
OBJECTIVE: To investigate the changes of pregnancy complicated by heart disease. METHOD: Clinical data of hospitalized pregnant women with heart disease, collected from 10 teaching hospitals in Shanghai during 1981-1995, were analysed retrospectively. RESULTS: 2,680 of 379,065 deliveries (0.71%) were complicated by heart disease during that period. There were a total of 121 maternal deaths, 15 of them due to heart disease, the mortality of heart disease was 0.56%, and the percentage in total maternal deaths was 12.40%. The incidence and mortality rates were similar in 1981-1985, 1986-1990, 1991-1995, but the percentage due to heart disease increased after the late 1980s. The rates of congenital heart disease increased and rhumatic heart disease decreased apparently, the ratio of the former to the latter was 1.76:1. The pregnancy induced hypertension heart disease, the peripartum cardiomyopathy and the miscellaneous heart disease all increased obviously during the 1990s. The heart functions of grade I and II accounted for a considerable proportion (85.45%), but the grade IV tended to increase during the 1990s. Heart failure occurred in 172 cases, with an incidence of 7.6%. The perinatal mortality rate was 7.76%. Cesarean sections were often performed in heart disease women. CONCLUSION: Pregnancy complicated by heart disease is still one of the major cause of maternal deaths up till now. More effective management should be adopted.
OBJECTIVES: To evaluate the effects of insulin-like growth factor-I (IGF-I) on the maturation, fertilization, and cleavage of murine oocytes. METHODS: Immature and mature murine oocytes were cultured alone or together with murine spermatozoa in vitro. Various concentrations of IGF-I were added to the media as experimental groups, and was not added as controls. Then the rates of maturation, fertilization and cleavage of murine oocytes were observed, and compared between the 2 groups. RESULTS: The rates of maturation, fertilization and cleavage of murine oocytes in experimental groups were significantly higher than those in the controls (P < 0.01 or P < 0.05). Unequal blastomeres and cell fragments were observed in both groups, but the differences during the same cultured period were not statistically significant (P > 0.05). CONCLUSIONS: IGF-I could induce murine oocyte maturation, promote its fertilization and cleavage in vitro, and do no harm to the fertilized oocytes and embryos.
From the view of the artificial intelligence, a new method of segmentation and display of human brain medical images is descrided. On the basis of the knowledge of brain anatomy and image processing, we have built the 3D knowledge mobel using frame as the main knowledge expression method. Under the guide of the knowledge, the main structures in the brain are segmented and displayed by means of the "inelligent ray-tracing".
In order to observe the effect of far infrared rays on the survival of skin flap, the following experiment was performed. Forty-eight SD rats were selected and divided into two groups. The rats received 0.3 w/cm2 radiation twice a day from 3 days before operation to 5 days after operation in the experimental group, while in the control group the rats received none before or after the operation. The flap was designed as 2 cm x 6 cm in the back of the rats with the pedicle caudalward. The microcirculatory changes of the flap were observed, and the survival area of the flap was calculated. The results showed that either in the proximal or in the distal part of the graft, in the experimental group, the mean opening rate, diameter and the flowing velocity of the microvessels were significantly higher than those in the control group (P < 0. 05). The mean rate of survival area of the experimental group (80.5%) was also higher than that of the control group (62.7%) (P < 0.01). It was suggested that radiation with far infrared rays could dilate the microvessels, improve the flap microcirculation, therefore, enhance the survival of the randomized skin flap.
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Specific families of transcription factors mediate events in the sequential maturation of distinct neuronal phenotypes. Members of one such family, the class IV POU domain transcription factor Brn-3.0, and two highly related factors Brn-3.1 and Brn-3.2, are differentially expressed in the developing and mature mammalian nervous system. The expression pattern of Brn-3.0 suggested that it has an important role in the development of sensory ganglia, as well as red nucleus, inferior olive, and nucleus ambiguus. Analysis of mice null for the Brn-3.0 locus shows that Brn-3.0 is required for the survival of subpopulations of proprioceptive, mechanoreceptive and nociceptive sensory neurons, where deletion of the gene affects neurotrophin and neurotrophin-receptor gene expression. Deletion of Brn-3.0 also alters either differentiation, migration or survival of specific central neuronal populations.
The distribution of the P2x2 purinoceptor subunit protein, which forms ATP-gated ion channels by homo- and hetero-multimeric assembly, was examined in the adult rat and guinea-pig cerebellum using two novel antisera generated against separate 18 amino acid sequences located in the predicted extracellular domain of this subunit. These antisera, the first available for labelling the P2x2R subunit protein, were validated by selective labelling of a fusion protein containing the target amino acid sequences, and in cerebellum, by peptide specific block of immunoreactivity and by comparison with the distribution of P2x2R mRNA. P2x2R-like immunoreactivity was seen in Purkinje cells, specifically the soma and dendrites, neurons in the granular and molecular layers and deep cerebellar nuclei. The identification of P2x2R-like immunoreactivity within the cerebellar neural circuitry is consistent with a role for extracellular ATP acting as a fast neurotransmitter in motor learning and coordination of movement. Additionally, labelling of neuroglia and fibre tracts supports a diverse role for extracellular ATP in CNS homeostasis.
Ataxia-telangiectasia (A-T) is a multisystem recessive disease characterized clinically by cerebellar ataxia, oculocutaneous telangiectasias, immunodeficiency, sensitivity to radiomimetic agents, and cancer predisposition. This pleiotropic disorder is caused by mutations in the ATM (mutated in A-T) gene, which is located in the human chromosomal region 11q22-q23. The ATM gene product is a member of a novel family of large proteins implicated in the regulation of the cell cycle and response to DNA damage. Heterozygosity for A-T was previously suggested to be associated with an increased risk of tumors, particularly female breast cancer. Because of loss of constitutional heterozygosity at 11q22-q23 is a frequent event in breast and other tumors, suggesting the presence of a tumor suppressor gene(s) in this region, we screened blood DNA samples from 88 unrelated breast cancer patients of Swedish cancer families for ATM mutations using single-strand conformation polymorphism analysis. All patients had a family history of tumors previously associated with A-T heterozygosity or homozygosity. We demonstrate the first three germ-line mutations in ATM identified by screening of breast cancer patients. Two mutations were previously found in A-T homozygotes and one mutation was a 1-bp insertion. All mutations were found in families with a large number of tumors, however, they did not cosegregate with malignancies. Although the proportion of A-T carriers in this sample seems to be higher than expected by chance, larger studies and pooled data sets will be required to establish that an A-T allele confers cancer susceptibility in heterozygotes.
The HIV-1 envelope glycoprotein gp120 displays inefficient intracellular transport, which is caused by its retention in the endoplasmic reticulum. Coexpression in insect cells (Sf9) of HIV-1 gp120 with calnexin has shown that their interaction was modulated by the signal sequence of HIV-1 gp120. gp120, with its natural signal sequence, showed a prolonged association with calnexin with a t1/2 of greater than 20 min. Replacement of the natural signal sequence with the signal sequence from mellitin led to a decreased time of association of gp120 with calnexin (t1/2 < 10 min). These different times of calnexin association coincided both with the folding of gp120 as measured by the ability of bind CD4 and with endoplasmic reticulum to Golgi transport as analyzed by the acquisition of partial endoglycosidase H resistance. Using a monospecific antibody to the HIV-1 gp120 natural signal peptide, we showed that calnexin associated with N-glycosylated but uncleaved gp120. Only after dissociation from calnexin was gp120 cleaved, but very inefficiently. Only the small proportion of signal-cleaved gp120 molecules acquired transport competence and were secreted. This is the first report demonstrating the effect of the signal sequence on calnexin association.
The Drosophila Appl gene encodes a transmembrane protein that is expressed exclusively in neurons. Amino acid comparisons show that APPL protein is a member of the amyloid precursor protein (APP)-like family of proteins. Similar to mammalian APP-family proteins, APPL is synthesized as a transmembrane holoprotein and cleaved to release a large secreted amino-terminal domain. Using immunocytochemical methods, we have analyzed the distribution of APPL in the Drosophila CNS. Surprisingly, although APPL is present in all neuronal cell bodies, the neurophil shows sterotypic differential distribution. Double-labeling experiments with different neuronal markers were used to distinguish between APPL associated with neuronal processes or extracellular matrix. The distribution of APPL protein produced from transgenes encoding wild-type (APPL), secretion-defective (APPLsd), and constitutively secreted (APPLs) forms was analyzed in an Appl-deficient background to determine which APPL form is associated with different neuropil regions. We found that APPLsd protein is enriched where APPL immunoreactivity coincides with neuronal processes. In contrast, APPLs preferentially localizes to those parts of the neuropil that show a diffuse APPL signal that rarely colocalizes with processes, and thus seems to be a component of the extracellular matrix. These data indicate that proteolytic cleavage and trafficking of APPL is differentially regulated in different neuronal populations. Through metamorphosis, APPL is especially abundant in growing axons and in areas where synapses are forming. Interestingly, in adult brains, APPL protein is enriched in the mushroom bodies and to a lesser extent in the central complex, structures involved in learning and memory.
Based on the investigation of the relation between gene expression and the usage of synonymous codons, a method of classifying and predicting the gene expression level is proposed which is called the Self-consistent Information Clustering (SCIC). Using the modified Codon Adaption Index (CAI) values, we have accomplished the linear regression analysis on the relation between base composition, base correlation and gene expression level in Escherichia coli and yeast. The assumption of Expression-Enhancing-Network Site (EENS) is proposed, the existence of which can be demonstrated by the linear equations between gene expression and base correlations in a codon, in adjacent codons and in non-adjacent codons. The modes of base correlation of E. coli and yeast which are important to gene expression have been found and listed in this paper.
Heterozygosity for ataxia-telangiectasia (A-T), a cancer-prone recessive syndrome, has been associated with an increased risk of breast cancer. The gene for A-T (ATM) is located at chromosomal region 11q22-q23, a region of frequent loss of constitutional heterozygosity in breast and other tumors. Loss of constitutional heterozygosity at 1lq22-q23 was found in 47% of informative cases in the series of primary tumors analyzed in this study. To investigate the role of ATM in breast cancer, we have determined the complete genomic organization of the gene, developed an exon-scanning PCR single-strand conformation polymorphism (PCR-SSCP) assay for mutation detection of ATM, and screened 38 consecutive breast tumors for mutations using both genomic DNA- and cDNA-based assays. In addition to common ATM polymorphisms detected both in the coding sequence and in flanking introns, seven unique SSCP alleles were identified in six tumor DNAs. Sequence analysis of these alleles revealed rive nucleotide substitutions that were predicted to change the encoded amino acid. However, PCR-SSCP and nucleotide sequencing analysis of the paired blood samples and of an extended sample size of a total of 224 chromosomes indicated that these SSCP patterns represent constitutional rare polymorphisms with a frequency between 0.005 and 0.023. Because the majority of A-T mutations are null mutations and none of the ATM alleles found in breast cancer samples would lead to the truncation of the translation product, we conclude that, in this initial sample of sporadic breast cancer patients, there was no evidence for an increased number of A-T carriers. In addition, because no somatic mutations were found, our study rules out the ATM gene as the frequently altered tumor suppressor gene at 11q23.
The neurally expressed genes Brn-3.1 and Brn-3.2 (refs 1-6) are mammalian orthologues of the Caenorhabditis elegans unc-86 gene that constitute, with Brn-3.0 (refs 1-3,8,9), the class IV POU-domain transcription factors. Brn-3.1 and Brn-3.2 provide a means of exploring the potentially distinct biological functions of expanded gene families in neural development. The highly related members of the Brn-3 family have similar DNA-binding preferences and overlapping expression patterns in the sensory nervous system, midbrain and hindbrain, suggesting functional redundancy. Here we report that Brn-3.1 and Brn-3.2 critically modulate the terminal differentiation of distinct sensorineural cells in which they exhibit selective spatial and temporal expression patterns. Deletion of the Brn-3.2 gene causes the loss of most retinal ganglion cells, defining distinct ganglion cell populations. Mutation of Brn-3.1 results in complete deafness, owing to a failure of hair cells to appear in the inner ear, with subsequent loss of cochlear and vestibular ganglia.
The transition metal complexes [eta(6)- (2beta-carbomethoxy-3beta-phenyltropane)]tricarbonylchromium (3) and [eta(6)-(2beta-carbomethoxy-3beta-phenyltropane)] [eta(5)-(pentamethylcyclopentadienyl)]ruthenium(II)triflate (4) were synthesized from 2beta-carbomethoxy-3beta-phenyltropane (2, WIN 35,065) to further elucidate the influence of substituents on the 3beta-aryl on the affinity of the ligand for cocaine-binding sites at the dopamine transporter. The compounds were tested for their ability to displace bound [(3)H]WIN 35,428 (5) from rat caudate putamen tissue and for their ability to inhibit [(3)H]dopamine uptake. The binding affinity for 3 was 2-fold greater than those observed for cocaine (1) and 2, while the binding affinity for 4 was found to be 100-fold less than those of 1 and 2. In addition, 3 was equipotent with 1 and 2 in [(3)H]dopamine uptake inhibition studies, while 4 was 10-fold less potent. The potencies of the complexes 3 and 4 correlated well with the structure-activity relationships of other 2beta-carbomethoxy-3beta-aryltropane derivatives. These data further support a pharmacophore model in which the region occupied by the aryl ring is a lipophilic pocket with electropositive character.
Neurons contain distinct compartments including dendrites, dendritic spines, axons and synaptic terminals. The molecular mechanisms that generate and distinguish these compartments, although largely unknown, may involve the small GTPases Rac and Cdc42, which appear to regulate actin polymerization. Having shown that perturbations of Rac1 activity block the growth of axons but not dendrites of Drosophila neurons, we investigated whether this also applies to mammals by examining transgenic mice expressing constitutively active human Rac1 in Purkinje cells. We found that these mice were ataxic and had a reduction of Purkinje-cell axon terminals in the deep cerebellar nuclei, whereas the dendritic trees grew to normal height and branched extensively. Unexpectedly, the dendritic spines of Purkinje cells in developing and mature cerebella were much reduced in size but increased in number. These 'mini' spines often form supernumerary synapses. These differential effects of perturbing Rac1 activity indicate that there may be distinct mechanisms for the elaboration of axons, dendrites and dendritic spines.