Biomedical subjects

L Li

Publications and source records attributed to L Li.

At least 685 records · Page 38Linked to original sources

PubMed2000

Change of apoptotic status in the human colorectal adenoma-carcinoma sequences and its correlation with carcinogenesis and prognosis.

OBJECTIVE: To assess apoptotic status during the development of colorectal cancer and its prognostic value. METHODS: The apoptotic frequency of 168 fresh adenocarcinoma specimens and primary cultured cells at 2, 12, 24 and 48 hours (9 normal mucosa, 4 adenomas and 9 adenocarcinomas) were measured by flow cytometry (FCM). Apoptotic indices (AI) in situ for 25 adenomas and 77 adenocarcinomas were visualized by TdT-mediated dUTP nick end labeling (TUNEL), Ki-s5 labeling indices (KI), bcl-2, bax, waf1 and p53 were immunostained with ABC method. RESULTS: The culture-related apoptosis at 24-48 hours in vitro was obviously decreased in cultured tumor cells when compared with mucosa cells. Spontaneous apoptosis in situ occurred more frequently in tumor with aneuploid type at late stage. There was positive relationship between apoptosis and proliferative activity, determined by both TUNEL and FCM methods. The well-differentiated or early stage lesions with intensive bcl-2/bax expression were significantly more likely to have low AI. p53 accumulation and waf1 depression were mainly related to KI, whereas bax and waf1 overexpression led to a comparatively higher AI/KI ratio. bcl-2 and KI were found to be independent risk factors. CONCLUSIONS: The data suggest that the depressed susceptibility to inductive apoptosis may contribute to the initial phase of tumorigenesis, and spontaneous apoptosis in vivo may serve as a marker of tumor progression. The bcl-2 and KI may be valuable in predicting prognosis in colorectal cancer.

Adenocarcinoma↗

PubMed2000

[Regulation of the expression and function of glucose transporter-1 by TGF-beta 1 and high glucose in mesangial cells].

OBJECTIVE: To study the effects of high glucose and transforming growth factor-beta 1 (TGF-beta 1) on the expression and function of glucose transporter-1 (GLUT1) in mouse mesangial cells. METHODS: Cultured mouse mesangial cells were used. The expression of GLUT1 mRNA was detected by Northern Blot; glucose uptake and its kinetics were determined with a 2-Deoxy-[3H]-D-glucose uptake assay. RESULTS: Mesangial cells exposed to enriched glucose medium (20 mmol/L) for 72 hours demonstrated a decrease in both GLUT1 mRNA and Vmax for uptake of the glucose analog, 2-deoxy-D-glucose (2DOG), as compared to mesangial cells cultured in physiologic glucose concentrations (5.5 mmol/L). In contrast, hypertonic mannitol had no effect on GLUT1 mRNA levels. TGF-beta 1 treatment for 10 hours stimulated 2DOG uptake, both in 5.5 mmol/L and 20 mmol/L glucose medium, by approximately 4.28-fold in a dose-dependent manner (2 ng/ml maximum). Kinetic analysis of 2DOG uptake revealed an increase in Vmax and a decrease in Km in the presence of TGF-beta 1. TGF-beta 1 also up-regulated the expression of GLUT1 mRNA in mesangial cells. The addition of anti-TGF-beta neutralizing antibody (30 micrograms/ml) in mesangial cells cultured in enriched glucose medium (20 mmol/L) led to a 40% decrease in 2DOG uptake. CONCLUSIONS: The expression of GLUT1 can be suppressed by exposure of mesangial cells to high glucose medium, which may serve as a protective mechanism against possible adverse effects of excessive glucose flux into cells. TGF-beta 1 stimulates glucose uptake by enhancing the expression and function of GLUT1 in mesangial cells. This effect is independent of the glucose milieu in the cultured medium.

Animals↗

PubMed2000

[Gibbs-Sampler approach for meta-analysis of multiple clinical trials using generalized linear model with random-effects].

OBJECTIVE: To investigate the use of the Gibbs-Sampler method in evaluating the relationship between clinic events and health risks in a meta-analysis of multiple clinical trials. METHODS: By using a generalized linear model with random-effects, Gibbs-Sampler technique was used in a meta-analysis of multiple clinical trials of angiotensin converting enzyme (ACE) inhibitors in patients with myocardial infarction (MI). RESULTS: When heterogeneity across different trials can not be ignored, compared with the classic method, the odds ratio of relative reinfarction risk estimated by the Gibbs-Sampler method would have less variation. The gain in the reduction of variation in estimate of the overall odds ratio was 9.52%. CONCLUSION: Implementation of the Gibbs-Sampler technique in meta-analysis of multiple clinical trials has the potential of reducing the inaccuracy caused by heterogeneity across trials.

Angiotensin-Converting Enzyme Inhibitors↗

PubMed2000

[Expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-3 protein in the implantation window phase of endometrium].

OBJECTIVE: To study the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-3 (TIMP-3) protein in the implantation window phase of endometrium and the role of MMP-9 and TIMP-3 on embryo implantation. METHOD: Immunohistochemical analysis was employed to demonstrate the population of the MMP-9 and TIMP-3 positive cell in endometrium of different phases of menstrual cycle. RESULTS: The positive cells of these soluble proteinases were found to be located in the endometrial epithelial cells and stromal cells. The quantitative analysis of MMP-9 and TIMP-3 proteins demonstrated an increase in staining exent from proliferative endometrium to the endometrium of implatation window phase, and an decrease to late-secretory endometrium in comparison with the implantation window phase. CONCLUSION: Expression of MMP-9 and TIMP-3 in the implantation window phases of endometrium is specific and may play an important role in regulating the depth of trophoblast invasion during embryo implantation and may be involved in endometrial stromal breakdown during menstruation.

Adult↗

PubMed2000

Successful treatment of invasive burn wound infection with sepsis in patients with major burns.

OBJECTIVE: To investigate the clinical characteristics of invasive burn wound infection with sepsis in patients with major burns and to summarize the successful experiences in the treatment of such patients. METHODS: Eight patients with major burns, complicated by invasive burn would infection and sepsis were consecutively admitted to our hospital from September 1997 to October 1998. Among them, 6 patients developed multiple organ dysfunction syndrome (MODS) and 2 developed septic shock. The plasma concentrations of IL-6, IL-8, TNF alpha and lypopolysaccharide (LPS) were assayed before and after surgical intervention, as well as when the patient's vital signs became stable. RESULTS: The patients' conditions usually deteriorated abruptly when extensive invasive burn wound infection emerged. While multi-microbial infection was usually found, Pseudomonas aeruginosa was the predominant bacteria isolated from the subeschar tissue. The plasma concentrations of IL-6, IL-8, TNF alpha and LPS before surgical intervention were significantly higher than those after surgical intervention (P < 0.05). The lowest levels of the inflammatory mediators were observed when the patients' conditions became stable, and the values were significantly lower than those before surgical intervention (P < 0.001). CONCLUSION: Since the main cause of burn wound sepsis is the presence of a large area of infected burn wound, they should be excised and covered as early as possible. LPS and pro-inflammatory mediators play an important role in the pathogenesis of burn sepsis. Although favorable results should be attributed to comprehensive treatment, we believe that early, aggressive and thorough surgical excision of infected burn wounds, followed by sound and complete coverage of the area, play a crucial role.

Adolescent↗

PubMed2000

[Analysis of the clinical characteristics and prognostic factors with primary normal-sized epithelial ovarian carcinoma syndrome].

OBJECTIVE: To investigate the clinical characteristics and prognostic factors of the primary normal-sized epithelial ovarian carcinoma syndrome. METHODS: The clinical records and pathologic slides of the 10 patients who satisfied the diagnostic criteria were retrospectively analyzed. RESULTS: The primary normal-sized epithelial ovarian carcinoma syndrome was detected in approximately 1.92% of the patients with the epithelial ovarian carcinoma. The age of the patients at diagnosis ranged from 43-70 years, with a median age of 57 years. The most common presentations included anorexia, increasing abdominal girth and awareness of abdominal or pelvic mass. During the exploratory laparotomy although we often discovered the diffuse metastasis throughout the abdominal and pelvic cavity, the ovaries both appeared normal size and had a fine granularity on the external surface. The survival time of patients with remained tumors smaller than 2.0 cm was longer than those with remained tumors larger than 2.0 cm, but the Log-rank evaluation indicated chi 2 = 0.61, P > 0.05. The patients who underwent postoperative chemotherapy have been noted to achieve some long-term survival. The median survival time was 20 months. CONCLUSION: The results predict that the size of the remained tumors may be one of the important factors to the survival and the disease is sensitive to the postoperative chemotherapy.

Adult↗

PubMed2000

[Clinical observation of taxotere in the treatment of advanced non-small-cell lung cancer and breast cancer].

OBJECTIVE: To observe the response rate and toxic reaction of taxotere in the treatment of advanced NSCLC and breast cancer. METHODS: A total of 24 cases was included in the study. Taxotere 75 mg/m2 i.v. drip for 1 hour; dexamethasone 8 mg bid 24 hour before taxotere, and continued for 3 days. For NSCLC, the taxotere was combined with DDP 90 mg/m2; for breast cancer, it was combined with ADM 40 mg/m2. The second treatment cycle was given after 3 weeks. At last 2 cycles were given. RESULTS: In 15 NSCLC patients, there were 6 PR, 7 NC and 2 PD. In 9 breast cancer patients, there were 2 CR, 6 PR and 1 NC. The major toxic reactions were neutropenia, diarrhea, fatigue, muscle pain and alopecia. Grade 3 and grade 4 neutropenia occurred in 9 and 4 cases, respectively. Decrease in hemoglobin and thrombocytopenia were mild. Diarrhea occurred in 19 cases and allergic reaction in 1 case. CONCLUSION: Taxotere is effective in the combined treatment of advanced NSCLC and breast cancer. The toxic reactions are tolerable.

Adult↗

PubMed2000

[A study on the changes of calcium signal in mast cells activated by antigen].

OBJECTIVE: To study the relationship between the calcium signal and activation of mast cells, laying the foundation for discovering a new potential theraputical target of signal transduction for type I anaphylactic diseases like asthma. METHODS: The fluorescent Ca2+ indicator Fluo-3 was used to observe and quantitate the calcium signal directly in activated RBL-2H3 mast cells by a flow cytometer and a confocal fluorescence microscope with laser. RESULTS: Antigens elicited significant increase in intracellular Ca2+ concentration ([Ca2+]i) and histamine release in sensitized cells, maximal [Ca2+]i increase being reached (525 +/- 42) nmol/L (n = 12) within 90-110 s after stimulation. Increase of fluo-3-fluorescence intensity was also observed with the confocal fluorescence microscope in cytosol. No significant increase of [Ca2+]i was observed after addition of EDTA in sensitized cells from an initial value of (79 +/- 3) nmol/L, to (80 +/- 4) nmol/L (P > 0.05, n = 12), but tyrosine phosphorylation was readily observed, and the indirect fluorescent intensity was significantly greater than that in the control group. No significant change of [Ca2+]i was observed with the addition of DNP-BSA after PMA (P > 0.05, n = 8). CONCLUSION: Calcium is involved in the activation process of mast cells stimulated by antigens.

Aniline Compounds↗

PubMed2000

[Primary drug-resistance of Mycobacterium tuberculosis in Shanghai, 1985-1995].

OBJECTIVE: To evaluate the problem of antituberculosis drug resistance in Shanghai, 1985-1995. METHOD: All of the strains of M. tuberculosis from new patients with pulmonary tuberculosis were tested for resistance to INH, RFP, EMB, SM, PAS, and KM by the absolute concentration method on Lowenstein-Jensen medium. RESULTS: The rates of primary drug resistance to SM, INH, RFP, PAS, and KM were 10.2%, 5.2%, 4.8%, 5.6%, and 3.9% respectively in 1985, and decreased to 4.8%, 4.8%, 4.3%, 3.6%, and 2.6% in 1995, while the rate of drug resistance to EMB was 1.3% in 1985, and increased to 3.3% in 1995. The primary resistance rate to one or more drugs was 15.6% in 1985 and 8.2% in 1995. The resistance rates to one, two, three, four, five, and six drugs were 5.9%, 4.6%, 2.5%, 1.5%, 0.4%, and 0.8% respectively in 1985, and decreased to 1.9%, 3.5%, 1.2%, 1.2%, 0.1%, and 0.4% in 1995. The resistance rates to INH and RFP were 1.9% in 1992-1993, 2.4% in 1994-1995. CONCLUSION: The primary drug resistance of M. tuberculosis in Shanghai, 1985-1995, was in a trend of decline, but multi-drug resistance is still seriously existed.

China↗

PubMed2000

[Thrombosis diseases and lung cancer].

OBJECTIVE: To increase the awareness of lung cancer complicating thrombosis disease(TD). METHODS: 20 cases of lung cancer with TD which were from 1,050 cases of lung cancer confirmed by pathology and cytology in 80's and 90's from a teaching hospital were retrospectively reviewed. RESULTS: Of the 20 cases, there were 8 cases complicated with deep venous thrombosis(DVT); 4 cases with pulmonary thromboembolism (PTE); 4 cases with DVT and PTE, and 4 cases with nonbacterial thrombotic endocarditis (NBTE). 6 cases of DVT/PTE occurred 2-6 months before detection of lung cancer, 3 cases of NBTE had brain and peripheral artery embolism 2-7 months before detection of lung cancer. The pathology of 12 cases of lung cancer with TD was adenocarcinoma. CONCLUSIONS: TD complicating lung cancer was not unusual and could be warning sign indicating occult lung cancer. Physician ought to alert to lung cancer or other cancers when facing aged healthy subjects of sudden TD under unknown origin. The most common physiologic type of lung cancer complicating TD was adenocarcinoma. Hypercoagulation state was the most important cause of TD complicating lung cancer.

Adult↗

PubMed2000

[Effect of 962 capsule on liver peroxidation, thymus and spleen index].

OBJECTIVE: To investigate the effect of 962 capsule, a TCM compound recipe, on liver peroxidation and weight of thymus and spleen (thymus index, spleen index) in aged rats, and observe the anti-oxidation effect and the influence on immunological function in rats. METHODS: Rats were divided into young control, aged model, positive control (piracetam), 962 middle (0.9 g/kg) dose and high dose (1.8 g/kg) groups, and the medicated rats used in each group were aged rats. All tested drugs were administered for 1 month by gastrogavage. The liver peroxidation was determined by thiobarbituric acid method. The thymus index and spleen index were determined by weighing method. RESULTS: Middle and high dose of 962 capsule attenuated liver peroxidation, their liver MDA lowered, increased the thymus index of aged rats. There was no effect on spleen index in all medicated groups. CONCLUSIONS: 962 capsule could reduce liver lipid peroxidation and increase thymus index. It is suggested that 962 capsule might be beneficial in retarding aging process.

Aging↗

PubMed2000

[Cardiac protective effects of tetramethylpyrazine in patients performed open-heart operation with cardiopulmonary bypass].

OBJECTIVE: To study the cardiac protective effects of tetramethylpyrazine (TMP) on patients undergoing cardiac operation. METHODS: Twelve patients with rheumatic heart disease were randomly divided into the TMP group and the control group, 6 in each group. The changes of hemodynamics, serum creatine phosphokinase (CPK), creatine phosphokinase isoenzyme (CPK-MB) levels were observed before, during and 72 hours after operation. RESULTS: The hemodynamic indexes in the TMP group were better than those in the control group after operation, the serum CPK, CPK-MB levels measuring 12 h, 24 h and 48 h after operation in the TMP group were lower than those in the control group. CONCLUSION: TMP has good cardiac protective function in patients undergoing cardiac operation in improving cardial function and reducing the release of myocardial enzymes.

Adult↗

PubMed2000

[Clinical observation on treatment of thyrotoxic exophthalmos with jiayanxiao plus tapazole].

OBJECTIVE: To study the therapeutic effect of Jiayanxiao (JYX) on thyrotoxic exophthalmos. METHODS: Forty-three patients were divided randomly into two groups, the 31 Patients in the treated group were treated with JYX plus tapazole, and the 12 patients in the control group were treated with prednisone plus tapazole. The therapeutic course of both groups was 3 months. The symptoms, eye signs, thyroxin level, and other routine laboratory parameters as well as the adverse effect of the therapy were observed and compared between two groups. RESULTS: The total effective rate in the treated group was 80.6%, which was obviously higher than that in the control group (50.0%, P < 0.05). Effect of the treated group in lowering degree of exophthalmos was superior to that of the control group. Effects in elevating vision, decreasing palpebral fissure altitude and lowering serum T3, T4 contents in the two groups were similar (P > 0.05). Moreover, the treated group showed better efficacy in improving clinical symptoms with less adverse effect. CONCLUSION: The therapeutic effect of JYX in treating thyrotoxic exophthalmos is ensured.

Adolescent↗

PubMed2000

[Clinical study on lixin pill in treating congestive heart failure].

OBJECTIVE: To observe the clinical therapeutic effect of Lixin Pill (LXP) in treating patients with congestive heart failure (CHF). METHODS: Ninety CHF patients with heart function of III-IV grade were randomly divided into 3 groups. Group A was treated with LXP, Group B with LXP plus small dosage of digoxin and Group C with ordinary dosage of digoxin. The therapeutic course for all the three groups was 1 month. Meanwhile, all patients were given ordinary dosage of diuretics, nitrates and angiotensin-converting enzyme inhibitors (ACEI) as basic therapy. RESULTS: The total effective rate in improving heart function in Group A, B and C was 83.3%, 93.3% and 90.0% respectively. In respect of improving myocardial contraction and dilation, the left ventricular ejection fraction (LVEF) was improved in all the three groups but was more superior in Group B (P < 0.05); the ratio of maximum blood flow between the advanced and the early diastolic stage (A/E) was improved, and the complicated supraventricular and ventricular premature contraction in Group A and B were reduced markedly after treatment (P < 0.01 or P < 0.05), and the effect in Group B was superior to that in Group A. CONCLUSION: Using LXP combined with small dosage of digoxin could improve the therapeutic effect in treating patients suffering from congestive heart failure.

Adult↗

PubMed2000

[The regulation of apoptosis by Bcl-2, bcl-X(L), Bcl-2alpha and Bax in chronic liver disease].

OBJECTIVE: To analyse the expression of Fas and Fas ligand (FasL) in patients of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma and to study the regulation of apotosis by Bax and Bcl-2 subfamilies. METHODS: The subfamily of Bcl-2 in the liver tissue was studied by immunohistochemistry. RESULTS: In 156 cases of chronic liver disease the expression levels of Fas and FasL in the chronic hepatitis group were significantly higher than that in hepatocellular carcinoma group(P < 0.01). The expression level of Bax subfamily in the chronic hepatitis group was significantly higher than that of Bcl-2 subfamily. CONCLUSION: It is suggested that Bcl-2 family is involved in the regulation of apoptosis in the chronic liver diseases.

Apoptosis↗

PubMed2000

[Early soft tissue coverage in treatment of severe open tibial fractures].

OBJECTIVE: To study the role of early soft tissue coverage in the treatment of severe open tibial fractures. METHODS: 76 open tibial fractures of type III b were divided into early group (0 - 7 days), subacute group (8 - 30 days), and late group(> 30 days) according to the coverage time of soft tissue. Complications such as infection and nonunion were compared. RESULTS: The infection and nonunion rates of early group were lower than those of the subacute and late groups. The average time to union of the early group was less than that of the subacute and late groups. CONCLUSIONS: Early soft tissue coverage can reduce the complications and the time to union of severe open tibial fractures. The time for "early" coverage should be limited in 1 week. The opportunity of nosocomial infection will increase after 1 week.

Adolescent↗

PubMed2000

[Anisodamine in prevention and treatment of sepsis of severely burned patients].

OBJECTIVE: To observe the preventive effect of anisodamine on possible sepsis of patients with major burns and the effect of anisodamine on patients with sepsis. METHODS: Forty-two patients with extensive burn admitted to our burn institute from April 1998 to November 1999 were divided randomly into two groups: treatment group (T group) and control group (C group). In the T group, all 20 patients received fluid resuscitation regimen with anisodamine, and in the C group, 22 patients received the regimen with no anisodamine. A tonometry catheter was positioned in the stomach, connecting with the automatic gas analysis machine (Datex-Engstrom Corporation, Dutch) for determining gastric intramucosal pH (pHi). The plasma concentrations of diamine oxidase (DAO) and endotoxin were measured. Correlation analysis between pHi, DAO and endotoxin were made respectively during early stage of postburn. All the parameters in 7 patients with sepsis before and after administration of anisodamine were compared with those in 6 patients with sepsis without use of anisodamine. RESULTS: The incidence of sepsis in the T group was lower (20.0%) than that in C group (40.9%). The gastric pHi value in the early period of postburn was significantly higher in the T group than in the C group (P < 0.05). Concurrently, the plasma concentrations of DAO and endotoxin were significantly lower in the T group than in the C group (P < 0.05 or 0.01). A significant negative correlation was seen between the gastric pHi and respective values of DAO, endotoxin (P < 0.05 or 0.01). There were a decrease in gastric pHi, and an increase in plasma DAO and endotoxin level in patients with septic episode; however all the parameters after administration of anisodamine were improved compared with those in septic patients without use of anisodamine. CONCLUSIONS: Intestinal ischemic injury plays an important role in provoking sepsis during early postburn period. Anisodamine is effective in restoring intestinal circulation both in the shock phase and after the development of sepsis.

Adolescent↗

PubMed2000

[Clinical use of acellular allogenic dermis or acellular porcine dermis with split-thickness autologous skin graft in 119 cases].

OBJECTIVE: To observe the effect of acellular allogenic dermis or acellular porcine dermis together with split-thickness autologous skin graft on coverage of deep burn wound and the wound of scar excision. METHODS: Acellular allogenic dermis or acellular porcine dermis produced by our unit, with split-thickness autologous skin graft, were used in repairing various wounds in 119 cases. The take rate of various wounds was compared, and the take rate of wounds in which autologous skin from different sites was used to cover either acellular allogenic dermis or acellular porcine dermis was also compared. Histological examination and follow-up were made in some cases. RESULTS: After tangential excision, eschar excision and scar excision, the wounds were covered with either allogenic acellular dermis or porcine acellular dermis and autologous split-thickness skin. The take rate was found to be (93.4 +/- 3.4)%, (92.1 +/- 4.6)%, (94.5 +/- 3.5)%, respectively. There was no a significant difference in take rate (P > 0.05). No significant difference in take rate between the transplantation of allogenic acellular dermis with autologous split-thickness skin and transplantation of porcine acellular dermis with autologous split-thickness skin was found (P > 0.05). When autologous split-thickness skin harvested from the trunk or extremities was used, the take rate was (93.1 +/- 4.8)%, (89.0 +/- 6.2)%, respectively, whereas the take rate was only about 70% - 80% when skin harvested from the scalp was used. The take rate was only about 40% when acellular allogenic dermis and autologous micro-skin graft were used to cover the wound. Histological examination 19 months after the transplantation revealed normal structure. Collagenous fibers were orderly arranged. There was no apparent hyperplasia of collagenous fibers. Hair follicles, sweat glands and other skin appendages were not found in the healed area. Grossly, no obvious pigmentation was seen, the surface was smooth with slight wound contraction, and the consistency was flexible. CONCLUSION: Acellular allogenic split-thickness dermis or acellular porcine split-thickness dermis with autologous split-thickness dermis for coverage of deep burn wound and the wound of scar excision is an ideal material.

Adolescent↗