Biomedical subjects
L Lauriola
Publications and source records attributed to L Lauriola.
Inhibitory activity of alpha-1-antitrypsin bound to human IgA.
Complexes between alpha-1-antitrypsin (alpha 1AT) and monoclonal IgA are regularly demonstrable in the plasma of myeloma patients. These alpha 1AT-IgA complexes, free of contamination by unbound alpha 1AT, are purified from 5 myeloma patients sera using salt-mediated hydrophobic chromatography. The complexes have a molecular weight greater than or equal to 400 000: this suggests that alpha 1AT is bound to di- or polymeric IgA. The alpha 1AT bound to IgA constitutes the 3.2, 3.5, 7.2, 8.5, and 24.6 per cent of the total alpha 1AT present in the 5 myeloma serum samples. There is a linear correlation between bound alpha 1AT concentration and IgA level in the range of the IgA concentrations considered (r = 0.988; p less than 0.05). Similar values are obtained quantitating bound alpha 1AT by radioimmunodiffusion technique or by determination of the trypsin-inhibiting capacity; this demonstrates that the bound alpha 1AT fully retains its inhibitory capacity. The biological significant of this binding phenomenon is discussed.
Echinococcus granulosus: specific quantification of the two most immunoreactive antigens in hydatid fluids.
Preparations of the two most immunoreactive Echinococcus granulosus antigens (antigens 4 and 5) from sheep hydatid fluid, purified by a simplified method, and monospecific antisera against antigens 4 and 5, prepared by a new procedure, were used to measure the antigenic concentrations of antigens 4 and 5 in swine, sheep, and human hydatid fluids from pulmonary or hepatic cysts. Two bovine samples and two commercial preparations were also tested. The concentration of both antigens was significantly higher in sheep and human hydatid fluids than in swine hydatid fluid. The antigenic content of the two bovine samples and of the two commercial preparations was below the sensitivity level of the method employed. Independently of the species tested, the amount of Echinococcus antigens was greater in hepatic than in pulmonary cysts. The ratio between the concentrations of antigens 4 and 5 was constant at about 1:10 in the samples from various organs and from different species. When there were enough samples for statistical analysis a linear correlation was found between the contents of these two antigenic components but there was none between the amounts of proteins and the antigenic concentrations in the single cysts. Sheep hydatid fluid must therefore be considered the best source of antigenic material for diagnostic purposes even though in human cysts the antigenic fraction is less contaminated by serum proteins. We describe a reliable method of standardising antigenic material for the immunodiagnosis of hydatid disease.
Echinococcus granulosus: preparation of monosepcific antisera against antigens in sheep hydatid fluid.
Explore the source record for details and available documents.
Tumors of the nasopharynx: review of 132 cases.
Explore the source record for details and available documents.
Clinical and pathogenetic relationship between chronic urticaria and morphofunctional modifications of the digestive tract.
Explore the source record for details and available documents.
[Cryptorchidism: a study of histological changes in undescended testis related to age].
A total of 46 testicular biopsies were examined to define the histological characteristics of undescended testicle. Nine of the biopsied organs were normally positioned testicles from deceased pediatric patients (aged 2 months-14 years), and the remaining 37 were undescended testes from children with various degrees of cryptorchidism (aged 18 months-14 years). As reported by others, the degree of histological damage was directly proportional to the age of the subject. Close correlation between histological findings and the level of descent was not observed.