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Biomedical subjects

L Latulippe

Publications and source records attributed to L Latulippe.

8 recordsLinked to original sources
PubMed1985

Studies on fibronectin in inflammatory vs non-inflammatory polymorphonuclear leucocytes of patients with rheumatoid arthritis. II. Synthesis and release of fibronectin in vitro.

We conducted studies dealing with the synthesis and release of fibronectin in vitro by polymorphonuclear leucocytes (PMNL). The specific purpose of our study was to look for any changes in these events as they happen in inflammatory vs non-inflammatory PMNL. We used PMNL isolated from the synovial fluid of patients with rheumatoid arthritis as a source of inflammatory cells and PMNL isolated from peripheral blood as a source of non-inflammatory cells. Marked differences were observed. Using 35S-methionine metabolic labelling and SDS-polyacrylamide gel analysis, we were first able to clearly observe an increased synthesis of fibronectin by inflammatory PMNL when compared to non-inflammatory PMNL. Furthermore, the release of fibronectin in vitro by these cells was increased by factors of up to 20 when compared to non-inflammatory peripheral blood PMNL. Experimental evidence was also obtained which strongly suggests that fibronectin exists in a stored form inside the inflammatory PMNL we used in this study. Finally, we observed that PMNL are capable of synthesizing a 95 kD gelatin binding protein which appears to be distinct from fibronectin.

Arthritis, Rheumatoid↗
PubMed1984

The role of fibronectin in the cryoprecipitation of monoclonal cryoglobulins.

We have recently reported that fibronectin is a component of mixed cryoglobulins and is responsible in large part for cryoprecipitate formation. We have now extended our studies to serum cryoprecipitates formed in the presence of monoclonal cryoglobulins in patients with lymphoproliferative diseases in order to investigate for a possibly similar influence of fibronectin on cryoprecipitation. Results have shown that these cryoprecipitates contain fibronectin in proportions varying between 10% and 14% of their total protein content. Furthermore, SDS-polyacrylamide gel electrophoresis revealed that next to the cryoglobulins, fibronectin constitutes the second major component of the cryoprecipitates. Depletion of the sera in fibronectin prior to exposure to 4 degrees C, markedly reduced cryoprecipitation of the cryoglobulins, whereas reconstitution in fibronectin restored it. These studies support and extend our previous findings showing the important influence exerted by fibronectin on the process of immunoglobulin cryoprecipitation.

Chemical Precipitation↗
PubMed1983

Psoriatic arthritis: risk factors for patients with psoriasis - a study based on histocompatibility antigen frequencies.

Histocompatibility antigen frequencies were studied in a group of 100 patients with psoriatic arthritis (PSA) and were compared to a group of 80 patients whose psoriasis was restricted to skin lesions (PSC). The antigens B13, BW57 (17), CW6 were significantly increased in PSC while BW57 (17), BW39, CW6 and CW7 were increased in PSA. No DR or MT antigen was elevated in frequency when compared to normal controls. The significant information which results from this study includes: 1) a failure to confirm previous reports on HLA-DR antigen increased frequencies; 2) an association, in this population, of BW39 with PSA and not BW38; 3) finding of a closer link of PSC and PSA with HLA-C rather than HLA-B antigens; 4) an estimate of relative risk for patients with PSC to develop PSA.

Adolescent↗
PubMed1979

[Psoriatic arthritis: analysis of 69 cases and review of the literature].

In a retrospective study 69 cases of psoriatic arthritis evaluated at the Centre hospitalier de l'université Laval (CHUL) between 1968 and 1977 were studied. Certain clinical and laboratory features (age, sex, nail involvement, date of onset of the cutaneous psoriasis, date of onset of the arthritis, and the presence or absence of rheumatoid factor and the HLA-B27 antigen) and the radiologic data were compared with findings previously described in the literature. With three exceptions the findings compared well: at CHUL (a) the frequency of the HLA-B27 antigen in patients with axial psoriatic arthritis was very low, (b) patients with synchronous psoriasis and arthritis were not more severely affected, and (c) certain radiologic signs that seem to be rare and have probably been overemphasized in the literature were not found.

Adult↗