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L L Ross

Publications and source records attributed to L L Ross.

42 records · Page 3Linked to original sources
PubMed1966

Radioisotopic studies of the binding, exchange, and distribution of 5-hydroxytryptamine synthesized from its radioactive precursor.

1. The synthesis, distribution, storage, and subsequent metabolism of 5-hydroxytryptamine (5-HT) produced in mice from the administration of its radioactive precursor, 5-hydroxytryptophan, has been investigated to form the basis for a similar study to be conducted by radioautography.2. Intravenous injection of the radioactive material was found to be essential for significant uptake of radioactivity by tissue. The duration of the period during which radioactive material was available for uptake by the tissue was 2 hr.3. The relative distribution of radioactivity in individual organs was studied and the radioactive compounds present in each were identified and quantitatively assayed. No unrelated routes of metabolism of the labelled material were found and radioautographic results may be interpreted in terms of the metabolic picture which emerged.4. Radioactive 5-HT was bound in tissues in preference to both its precursor, and metabolite, 5-hydroxytryptamine-O-glucuronide, and the radioactive 5-HT produced in vivo after the injection of labelled 5-hydroxytryptophan probably entered and labelled the endogenous 5-HT pool.5. Fixatives prepared with buffer solutions made hypertonic by the addition of sucrose could rapidly and effectively halt the movement of radioactive 5-HT out of tissues and prevent its subsequent extraction during histological processing.6. It is concluded that localization of sites concerned with 5-HT metabolism by means of radioautography is feasible and the 5-HT so localized will probably reflect the physiological compartmentalization of the amine.

5-Hydroxytryptophan↗
PubMed1966

Location of sites of 5-hydroxytryptamine storage and metabolism by radioautography.

1. A technique for the radioautographic identification, localization, and study of the turnover of cellular depots of 5-hydroxytryptamine (5-HT) has been evaluated. A light microscopic survey was made of the uptake and turnover of 5-HT, synthesized in vivo from its administered tritium-labelled precursor, 5-hydroxytryptophan (5-HTP).2. 5-HT was taken up rapidly and retained for long periods of time by adrenal medullary chromaffin cells, gastric enterochromaffin cells, blood platelets, thyroid parafollicular cells, beta cells of pancreatic islets, mast cells, and septal cells of the lung. Reticulo-endothelial cells of liver and spleen took up radioactive 5-HT more slowly but also retained it for days.3. Specific uptake, and a rapid turnover of radioactive 5-HT, was found in pancreatic exocrine cells, neurones of the superior cervical ganglion, terminal axons of gastro-intestinal myenteric plexus, carotid body cells, and pinealocytes.4. A supranuclear localization of radioactive 5-HT in renal proximal tubular cells may represent tubular secretion of 5-HT.5. The localization of 5-HT by radioautography generally corresponds to that found by formaldehyde-induced fluorescence. It makes possible the study of 5-HT turnover and is capable of greater resolution than the fluorescence technique. Freeze-drying may also be avoided.

5-Hydroxytryptophan↗