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L L Kisselev

Publications and source records attributed to L L Kisselev.

At least 73 records · Page 4Linked to original sources
PubMed1988

A general approach to the localization of antigenic determinants of a linear type in proteins of unknown primary structure.

A method is proposed which permits the localization of antigenic determinants of a linear type on the polypeptide chain of a protein molecule of unknown primary structure. An antigen modified with maleic anhydride at the amino-terminal groups and at the epsilon-NH2 groups of lysine residues was subjected to partial enzymic digestion, so that the antigenic protein had, on average, less than one cleavage site per polypeptide chain. The resultant ends were labeled with 125I-labeled Bolton and Hunter reagent and the maleic group removed. The detection of the two larger labeled fragments (a longer one which still could bind to a monoclonal antibody and a shorter one which was incapable of binding) made it possible to determine the distance from the antigenic determinant to the C-terminus of the polypeptide chain. The position of the antigenic determinant could be established in more detail using partial chemical degradation of the original antigen using information about the maximal length of a fragment which has lost its ability to interact with the monoclonal antibody. The method has been applied to bovine tryptophanyl-tRNA synthetase (EC 6.1.1.2).

Amino Acid Sequence↗
PubMed1988

Expression of the protooncogenes in psoriatic lesions.

The expression of cellular protooncogenes in psoriatic lesions was studied. RNA isolated from involved skin of psoriatic patients was subjected to dot-blot hybridization with cloned viral Ki-ras, myc, abl, fos, src, erbB, mos, sis, fes, and yes oncogenes. RNA isolated from the aortal epithelium, peripheral blood lymphocytes, and skin of the nonpsoriatic patients was used as control. The results indicate that the expression of Ki-ras, myc, fos, and abl genes is elevated in psoriatic lesions. The data of this work suggest that the activity of cellular oncogenes may play an important role in the development of psoriasis.

Gene Expression Regulation↗
PubMed1985

Nucleotide sequences in human chromosomal DNA from nonhepatic tissues homologous to the hepatitis B virus genome.

DNA extracted from human nonhepatic tissues (placenta and kidney) have been digested with restriction endonucleases and examined by the Southern procedure with cloned 32P-labelled DNA of hepatitis B virus (HBV). In placental DNAs of women with the history of a hepatitis B infection and in one out of four cases of patients with no known HBV exposure or manifestation, HBV-related chromosomal nucleotide sequences were detected. The integration of HBV-related sequences was observed also in human kidney DNA. Moreover, in the placenta of women who had hepatitis B infection prior to delivery, unusual unintegrated forms of HBV have been found. We conclude that HBV sequences can be found not only in hepatic tissue but also in placental and kidney DNA, both of HBV-exposed and in one case even of a nonexposed patient.

Base Sequence↗
PubMed1985

Human genome: proto-oncogenes and proretroviruses.

A brief review of the studies undertaken at the Laboratory for Molecular Bases of Oncogenesis (Institute of Molecular Biology, Moscow) till middle of 1984 is presented. The human genome contains multiple dispersed nucleotide sequences related to the proto-oncogene mos and to proretroviral sequences in tight juxtaposition to each other. From sequencing appropriate cloned fragments of human DNA in phage and plasmid vectors it follows that one of these regions, NV-1, is a pseudogene of proto-mos with partial duplications and two Alu elements intervening its coding sequence, and the other, CL-1, seems to be also a mos-related gene with a deletion of the internal part of the structural gene. CL-1 is flanked by a proretroviral-like sequence including tRNAiMet binding site and U5 (part of the long terminal repeat). The proretroviral-like sequences are transcribed in 21-35S poly(A)+RNA abundant in normal and malignant human cells. Two hypotheses are proposed: endogenous retroviruses take part in amplification of at least some proto-oncogenes; proto-oncogenes are inactivated via insertion of movable genetic elements and conversion into pseudogenes. Potential oncogenicity of a normal human genome undergoes two controversial influences: it increases due to proto-oncogene amplification and decreases due to inactivation of some of them.

Base Sequence↗
PubMed1984

The secondary structure of oocyte and somatic 5S ribosomal RNAs of the fish Misgurnus fossilis L. from nuclease hydrolyses and chemical modification data.

We have studied the accessibility of 5'- 32P labeled oocyte and somatic 5S rRNAs from the fish Misgurnus fossilis L. to S1, T1 and cobra venom nucleases and have found that the cleavage sites of 5S rRNAs closely related in primary structures differ in these molecules. The data of nuclease hydrolyses revealed the existence of two conformers corresponding to renatured and partially denatured somatic 5S rRNA and capable of mutual interconversions. The exposed cytosine residues were located in oocyte and somatic 5S rRNAs converted into uridine ones by sodium bisulfite treatment. The data have been used to construct the secondary structure models of somatic and oocyte 5S rRNAs by means of specially devised computer program. These models differ in their 5'-halves which contain all the nucleotide substitutions in the primary structure, all differences in location of the exposed cytosine residues, and finally, in the cleavage pattern by the nucleases used.

Animals↗
PubMed1984

The coding region of the human c-mos pseudogene contains Alu repeat insertions.

We have determined the nucleotide sequence of an 841-bp fragment derived from a segment of the human genome previously cloned by Chumakov et al. [Gene 17 (1982) 19-26] and Zabarovsky et al. [Gene 23 (1983) 379-384] and containing regions homologous to the viral mos gene probe. This sequence displays homology with part of the coding region of the human and murine c-mos genes, contains several termination codons, and is interrupted by two Alu-family elements flanked by short direct repeats. Probably, the progenitor of the human c-mos gene was duplicated approximately at the time of mammalian divergence, was converted to a pseudogene, and acquired insertions of two Alu elements.

Base Sequence↗
PubMed1983

Tight linkage of retroviral-like sequences to a variant human c-mos gene in the human genome.

Chumakov et al. [Gene 17 (1982) 19-26] identified in the human gene library a number of recombinant phages that possess a homology to the v-mos gene. Here we report the unusual structure of one of these recombinants, lambda gp5. The 14.3-kb stretch of human DNA from this phage contains at least three regions of homology to the v-mos gene, together with multiple copies of Alu-family repeats. Moreover, we have shown the presence of retrovirus-related sequences in the close vicinity of the mos-homologous regions. These data point to the possibility of involvement of retrovirus in the process of c-mos gene amplification during the formation of a multigene family.

Gene Amplification↗
PubMed1983

Nucleotide sequence of the anemic pigeon alpha-globin gene. Structural rearrangements in the cloned cDNA.

Pigeon alpha-globin cDNA, previously cloned into the PstI site of pBR322 to yield pGP22 (Scobeleva et al., 1981), was mapped and sequenced. The insert was found to have an unusual structural organization, with 33 bp corresponding to the 5'-untranslated region of mRNA located roughly at the middle and flanked downstream by the 81 triplets coding for the N-terminal half of the alpha-globin chain. Moreover, 25 triplets identical to the 3'-terminal region of the insert were located upstream from the 5'-untranslated region and were followed by the coding sequence of 82 to 134 triplets. The last seven codons from 135 to 142 triplets were missing. The structural rearrangements in the cloned cDNA are discussed. The nucleotide sequence of alpha-globin mRNA from anemic pigeon was determined; it differs remarkably from those for chicken and duck, although homologies can easily be found. The predicted amino acid sequence of pigeon anemic alpha-globin is given.

Anemia↗
PubMed1982

Human nucleotide sequences related to the transforming gene of a murine sarcoma virus: studies with cloned viral and cellular DNAs.

A recombinant plasmid, pI26, has been constructed by cloning into pBR322 a transforming gene of murine sarcoma virus (a Moloney strain, clone 124, MSV) synthesized by detergent-treated virions. From this plasmid a XbaI-HindIII fragment has been isolated which contains only mos-specific sequences. This mos-specific probe has been used for screening a human gene library cloned in bacteriophage lambda Charon 4A. Of these, 19 clones have been isolated containing mos-related sequences. By physical mapping and molecular hybridization it has been shown that these sequences are neighboured by DNA regions related to Moloney murine leukemia virus. Recombinant phages have also been found containing human inserts related to MLV, not to the mos gene. The possible existence of murine-like endogenous retroviruses in the normal human genome, including that of a sarcoma type, is discussed. By Northern blotting, expression of the cellular c-mos gene has been detected in mouse liver treated with a hepatocarcinogen. The general significance of the suggested model for evaluating the relationship between chemical carcinogenesis and oncogene expression is discussed.

Animals↗