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L L Grinius

Publications and source records attributed to L L Grinius.

24 records · Page 2Linked to original sources

[Stimulating action of polyphosphates on peptide formation from glycine and phenylalanine amides under abiogenic conditions].

Synthesis of peptides during polymerization of GlyNH2 and PheNH2 has been demonstrated by means of gel-chromatography and thin-layer chromatography. The optima of pH and temperature have been estimated for the reaction. Grem's salt, tripolyphosphate and pyrophosphate were shown to cause the stimulatory effect on the peptide formation, but there was no correlation between the yield of the peptides and the hydrolysis of the polyphosphates. On the basis of the experimental data, it has been concluded that hydrolysis of polyphosphates is not an energy source for the polymerization of GlyNH2 and PheNH2. Therefore, polyphosphates cause catalytic effect on the peptide synthesis from the amides of amino acids in homogeneous medium.

Catalysis↗

[The role of ATP and membrane potential in the penetration of phage T17 DNA into the cell during infection].

The role of ATP and membrane potential in phage T7 DNA injection into E. coli during infection has been studied. Entrance of phage T7 genes of class II and III was shown to be prevented by arsenate, indicating the requirement for phosphorylated macroergs in the phage DNA injection. The injection process was also inhibited by exposition of the cells to the uncoupler of oxidative phosphorylation. Dependence of the injection efficiency on the membrane-potential value has been shown to be sigmoidal, which suggests a regulatory role of the membrane potential in phage T7 DNA injection from the virion into the host cell.

Adenosine Triphosphate↗