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L Kullman

Publications and source records attributed to L Kullman.

16 recordsLinked to original sources

20th century climate warming and tree-limit rise in the southern Scandes of Sweden.

Climate warming by ca. 0.8 degree C between the late-19th and late-20th century, although with some fluctuations, has forced multispecies elevational tree-limit advance by > 100 m for the principal tree species in the Swedish part of the Scandinavian mountain range. Predominantly, these processes imply growth in height of old-established individuals and less frequently upslope migration of new individuals. After a slight retardation during some cooler decades after 1940, a new active phase of tree-limit advance has occurred with a series of exceptionally mild winters and some warm summers during the 1990s. The magnitude of total 20th century tree-limit rise varies with topoclimate and is mainly confined to wind-sheltered and snow-rich segments of the landscape. Thickening of birch tree stands in the "advance belt" has profoundly altered the general character of the subalpine/low alpine landscape and provides a positive feedback loop for further progressive change and resilience to short-term cooling episodes. All upslope tree-limit shifts and associated landscape transformations during the 20th century have occurred without appreciable time lags, which constitutes knowledge fundamental to the generation of realistic models concerning vegetation responses to potential future warming. The new and elevated pine tree-limit may be the highest during the past 4000 14C years. Thus, it is tentatively inferred that the 20th century climate is unusually warm in a late-Holocene perspective.

Climate↗

Probing sugar translocation through maltoporin at the single channel level.

Sugar permeation through maltoporin of Escherichia coli, a trimer protein that facilitates maltodextrin translocation across outer bacterial membranes, was investigated at the single channel level. For large sugars, such as maltohexaose, elementary events of individual sugar molecule penetration into the channel were readily observed. At small sugar concentrations an elementary event consists of maltoporin channel closure by one third of its initial conductance in sugar-free solution. Statistical analysis of such closures at higher sugar concentrations shows that all three pores of the maltoporin channel transport sugars independently. Interestingly, while channel conductance is only slightly asymmetric showing about 10% higher values at -200 mV than at +200 mV (from the side of protein addition), asymmetry in dependence of the sugar binding constant on the voltage polarity is about 20 times higher. Combining our data with observations made with bacteriophage-lambda we conclude that the sugar residence time is much more sensitive to (and is decreased by) voltages that are negative from the intra-cell side of the bacterial membrane.

Bacterial Outer Membrane Proteins↗

Evaluation of single-tooth restorations on ITI dental implants. A prospective study of 29 patients.

From January 1992 to March 1997, a total of 30 ITI hollow cylinder implants were installed to replace lost single maxillary incisor teeth. Conical abutments with cemented all-ceramic crowns were used in 10 cases and the Octa-abutment with screw-retained metallo-ceramic crowns in 19 cases. One implant failed due to postoperative infection, and was extracted a month after installation. After a mean observation time of 3.4 years, the cumulative success rate is 96.7%. Only minor bone loss has occurred around the implants, and there have been no other complications to date. The implant system fulfills the requirements for good function and esthetics.

Adult↗

Methods of reducing observer variation in age estimation from panoramic radiographs.

OBJECTIVE: To compare inter- and intra-observer variation in age estimation using subjective and objective methods. METHODS: Fifty-six panoramic radiographs of patients aged 13 to 25 years were assessed subjectively twice by four observers for the stage of root development of the lower third molars on a seven-point scale. Two of the observers were calibrated before the first evaluation, and the other two before the second. The same observers also digitized predefined reference points in a subsample of the third molars ten times by means of a computer-based digitizer system. Observer variation in classifying different root stages and in digitizing coordinates and measuring root lengths were studied. RESULTS: Significant systematic differences existed between two of the observers in the subjective assessment. One non-pre-calibrated observer changed her pattern most. Scattergrams from the digitizer measurements showed a good precision within and between observers with no significant differences in calculating root lengths. When, however, the teeth were analysed separately, some significant differences were found. CONCLUSIONS: The overall observer agreement was best using the objective method on several teeth. With only one tooth there were some significant differences. Subjective evaluation was improved by prior calibration.

Adolescent↗

Accuracy of two dental and one skeletal age estimation method in Swedish adolescents.

The paper concerns the accuracy of two dental methods of age estimation based on the radiographic appearance of the root of the lower third molar. The first dental method tested was traditional with a subjective assessment of the root development stage and the second was a new method with a metric measurement of formed root length. Since previous studies have shown a relatively low precision for dentally based age estimation methods during adolescence, an additional independent indicator of chronological age was employed, namely skeletal maturity according to the atlas and method of Greulich and Pyle (Radiographic Atlas of Skeletal Development of the Hand and Wrist Stanford University Press, CA, 1959). The material was Swedish adolescents aged 12-19 years. It was found that all methods gave an overestimation of chronological age, with the highest overestimation, more than 1 year, for the two dental methods. A stepwise multiple regression analysis indicated that skeletal age alone explains 48% of the variation in estimated chronological age. It may be concluded that the accuracy of age estimations based on the lower third molars is quite uncertain during adolescence. Up to 18 years, it is preferable to use skeletal age alone as a predictor. Only a small increase in the explanation coefficient of age variation (3-4%) could be seen if digitized or subjectively estimated root lengths were added as predictors to the skeletal estimation.

Adolescent↗

Computerized measurements of the lower third molar related to chronologic age in young adults.

Chronologic age is an utmost important birth record in many industrialized Western countries. When the date of birth is unknown, there is a demand for establishing the chronologic age. Dental methods for age estimations are considered more reliable than most other methods by the majority of researchers and are among the most commonly used means to determine age. Traditionally, these methods have most often been based on subjectively estimated, radiographic stages of tooth development. In the present study metric measurements of the length of the forming root have been used and are compared with one standard method. The formed part of the root of the lower third molar as seen in a panoramic radiograph was digitized. Data were directly stored in a computer for subsequent statistical analysis. The technique of collecting data with the aid of a computer had some statistical advantages. The precision of age estimations was, however, slightly inferior to the standard method.

Adolescent↗

Accuracy and precision in some dental radiographic methods. A methodological study with special considerations in age estimation in juveniles.

A computer method enabling metric measuring in radiographs has been presented. The measurements are time saving and precise and accurate measurements can be made. The statistical analysis can be performed based on a ratio scale, allowing more reliable and conclusive statistics. The most useful field for the presented method is offered in epidemiology where great materials are being handled. Since small changes can be studied, the method can be useful in studying marginal bone level changes in large populations. Or marginal bone levels around implants. In age estimation the digitizer method has no direct valuable application during adolescence, when the lower third molar is used. Here the present series of studies showed the traditional age estimation method and the digitizer method to be fairly unprecise with rather large systematic error. The skeletal age estimation method according to Greulich and Pyle was found to be more accurate and useful during the ages 14 up to 18 years. In younger children, where more parameters or teeth are available, there may be advantages to use a digital dental method instead of a traditional dental one.

Adolescent↗

Computer registration of missing persons. A case of Scandinavian cooperation in identification of an unknown male skeleton.

A case is described where the identity of an unknown body was established several years after death and despite the fact that the body was found in a foreign country. This was made possible due to the established Interpol routines and the good cooperation between the authorities involved in the Scandinavian countries. A significant role was also played by a computerized register with antemortem dental findings of missing Swedish citizens.

Dental Records↗

International co-operation in a dental identification.

A case of international identification of an unknown male, where the teeth were essential in establishing identity is described. A noteworthy aspect of the procedure was the teamwork between the police and other authorities, and the forensic odontologist and medical examiners in Albania and Sweden. In Albania a forensic medical examiner handled the dental examination, since forensic odontology is not yet acknowledged. The importance of having dental and medical ante-mortem registers for missing persons in every country is stressed. This is best administered in co-operation with the police authorities.

Adult↗

Root development of the lower third molar and its relation to chronological age.

Methods based on radiological, distinguishable stages of tooth formation can be used to estimate chronological age in young persons. The best precision and accuracy of these methods of age estimation is obtained when many teeth are under development and when the individual growth rate is rapid. That is during early years-in childhood. After an age of about 14 years it becomes more difficult since all permanent teeth but the wisdom teeth have completed their development and only these remain to be used for age estimation. The aim of this study was to examine the radiological development of the root of the mandibular third molar and to explore its usefulness in age estimation. Also the differences between two independent observers were studied. The observers classified the development of the mandibular third molars, as seen in a panoramic radiograph, into seven defined stages. The two observers agreed in about 84% of the cases in their registration of the different stages, but a significant difference was found between the observers. The mineralisation of the third molar's root was found to start at the age of 15 years and the root was fully formed at about 20 years. Some minor differences were found between sexes in the time sequence of the mineralisation. The study also shows that there is a rather low precision in the age estimation with the method used. In general, a standard deviation of about 1 to 2 years was found around the mean age of the different developmental stages.

Adolescent↗

Value of the frontal sinus in identification of unknown persons.

The reliability of comparing ante- and post-mortem radiographs of the frontal sinus as an identification medium for unknown corpses was tested by means of two radiographs each of 99 individuals. Three independent observers attempted to blind match each pair of radiographs. The results were almost 100% successful and showed that the radiographic image of the frontal sinus, even with varying beam angulation and radiological inexperience of observer is an effective identification medium.

Forensic Dentistry↗

Characterization of glucocorticoid receptors in S49 mouse lymphoma cells by affinity labeling with [3H]dexamethasone 21-mesylate.

Glucocorticoid receptors in wild type and mutant S49 mouse lymphoma cells were affinity labeled with [3H]dexamethasone 21-mesylate and analyzed directly by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weight of receptors in cytosol from wild type cells and nuclear transfer decreased (nt-) mutants was 97,000 (97 kDa). The molecular weight of receptors in cytosol from nuclear transfer increased (nti) mutants was 48 kDa. The 97 kDa receptor in cytosol from wild type cells was digested by chymotrypsin to a 40 kDa steroid-binding receptor fragment but the 48 kDa receptor in cytosol from nti mutants was resistant to digestion by chymotrypsin. In addition to the 48 kDa receptor, cytosol from nti mutants contained 40 and 18 kDa receptor fragments. Cytosol from the nt- mutants also contained 18 kDa receptor fragments. The 40 and 18 kDa receptor fragments were present in multiple subclones of a nti mutant cell line. Formation of these receptor fragments was not prevented by protease inhibitors and was not increased by extended incubation of cytosol samples. Both 48 and 40 kDa forms of the receptor, but not the 18 kDa form, could be activated and bound by DNA-cellulose.

Affinity Labels↗

Computerized registration of epidemiological data from intraoral radiographs.

Software was developed for graphic registration of epidemiological dental data from intraoral radiographs. The measured variables included marginal bone level in relation to tooth and root length as well as proximal caries and restoration areas. Caries and peridontitis progress nowadays slowly in large populations. Registration methods must, therefore, offer high precision. The hard and software were first tested. After that the method's reliability or precision was studied. The precision of measuring marginal bone level as a per cent of total tooth or root length was found to vary between 0.3-4.3. The corresponding precision of measuring a caries area in relation to the whole crown area was 1.4%. Precision was also divided into its components and analysed in more detail. All registered tooth variables except the angle between the proximal marginal bone and the root surface gave satisfactory results indicating good reliability.

Adult↗