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Biomedical subjects

L KAUFMAN

Publications and source records attributed to L KAUFMAN.

At least 19 recordsLinked to original sources
PubMed1964

FLUORESCENT-ANTIBODY STUDIES OF THE MYCELIAL FORM OF HISTOPLASMA CAPSULATUM AND MORPHOLOGICALLY SIMILAR FUNGI.

Kaufman, Leo (Communicable Disease Center, Atlanta, Ga.), and Brenda Brandt. Fluorescent-antibody studies of the mycelial form of Histoplasma capsulatum and morphologically similar fungi. J. Bacteriol. 87:120-126. 1964.-Fluorescent-antibody reagents were developed to differentiate the mycelial form of the pathogen, Histoplasma capsulatum, from several morphologically similar but saprophytic species of the genera Sepedonium and Chrysosporium. Cross-staining and adsorption procedures revealed antigenic relationships among certain species of these three genera and other fungi. The significance of these observations to the diagnostician and taxonomist is discussed.

Coloring Agents↗
PubMed1963

SEROLOGICAL CHARACTERIZATION OF PATHOGENIC FUNGI BY MEANS OF FLUORESCENT ANTIBODIES. I. ANTIGENIC RELATIONSHIPS BETWEEN YEAST AND MYCELIAL FORMS OF HISTOPLASMA CAPSULATUM AND BLASTOMYCES DERMATITIDIS.

Kaufman, Leo (Communicable Disease Center, Atlanta, Ga.) and William Kaplan. Serological characterization of pathogenic fungi by means of fluorescent antibodies. I. Antigenic relationships between yeast and mycelial forms of Histoplasma capsulatum and Blastomyces dermatitidis. J. Bacteriol. 85:986-991. 1963.-Four antiglobulins prepared against the yeast and mycelial forms of Histoplasma capsulatum and Blastomyces dermatitidis were labeled with fluorescein isothiocyanate. Cross-staining and adsorption techniques enabled the discernment of five antigenic factors in the fungi studied. The yeast-phase cells of H. capsulatum and B. dermatitidis contained the respective distinct antigens B and D, and a common factor E. In addition, the yeast-phase cells of these two species shared antigen C with the mycelial elements of H. capsulatum. The mycelial cells of H. capsulatum and B. dermatitidis shared antigen A with the yeast-phase cells of these two organisms and contained no distinct antigens.

Antigens↗
PubMed1961

Preparation of a fluorescent antibody specific for the yeast phase of Histoplasma capsulatum.

Kaufman, Leo (Communicable Disease Center, Atlanta, Ga.) and William Kaplan. Preparation of a fluorescent antibody specific for the yeast phase of Histoplasma capsulatum. J. Bacteriol. 82:729-735. 1961.-Rabbit anti-Histoplasma capsulatum globulin was conjugated with fluorescein isothiocyanate. This labeled antibody, in addition to staining six strains of H. capsulatum, exhibited strong cross-staining with ten strains of Blastomyces dermatitidis and numerous other species of fungi. Adsorption of the labeled antiglobulin either twice with cells of B. dermatitidis or twice with cells of Coccidioides immitis and once with cells of B. dermatitidis resulted in a good staining fluorescent antibody reagent, specific for yeast phase cells of H. capsulatum. Attempts to define the specific labeled antibody by the Ouchterlony procedure were unsuccessful. The specific conjugate did not give a complement fixation titer with yeast phase cells of B. dermatitidis or with coccidioidin. It did, however, show a 1:16 complement fixation titer with homologous yeast phase cells and a weaker reaction with histoplasmin.

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