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Biomedical subjects

L K Shataeva

Publications and source records attributed to L K Shataeva.

28 records · Page 2Linked to original sources
PubMedDate not supplied

[Structure and sorption properties of regenerated hemosorbent SCN].

Stepwise desorption of protein components from the hemosorbent SCN after hemoperfusion was studied, and their molecular weights were determined. It was found that hard alkaline regeneration and oxidation with nitric acid do not affect the hemosorbent structure, composition and potentiometric characteristics. The regenerated hemosorbent can be successfully used for adsorbtion of extraneous low- and medium-molecular weight components to clarify microbiological liquids.

Adsorption↗
PubMedDate not supplied

[Sorption properties of carboxyl cation exchangers with a bacteriostatic effect].

Sorption properties of new carboxyl cation exchangers containing components of salicylic acid (CST and CMTS) and benzoic acid (CBT and CMTB) were examined with respect to large organic ions. Such cation exchangers were shown to have greater permeability for high molecular weight proteins that sorbents of the Biocarb type. Bacteriostatic properties of the above cation exchangers were studied. With an increase in the content of the bactericidal component the bacteriostatic effect of the cation exchangers on Escherichia coli and Staphylococcus aureus enhanced. The cation exchangers CST and CMTS showed a greater bacteriostatic effect than those CBT and CMTB.

Benzoates↗
PubMedDate not supplied

[Isolation and purification of urokinase].

An improved procedure is developed for isolation of urokinase from human urine by means of ion exchange on cyrboxyl cationite SMT at pH 4.7. with subsequent stepwice elution by phosphate buffer, rH7.0. Depigmentation of the urine was achieved by pretreatment with an anionite FAF. Chromatographically pure preparation of urokinase was obtained as shown by gel filtration through Sephadex G-100 and G-150.

Anion Exchange Resins↗
PubMedDate not supplied

[Effect of FAF anionite swelling on its sorption properties].

Experiments were carried out to clarify the effect of swelling coefficients of various samples of the polycondensation anion exchange resin FAF in the C1 form on its sorption properties. The anion exchange resin was used to eliminate contaminating proteins and low molecular weight agents from the allantois fluid and liver cultured cells of pigs. Thermal treatment of the anion exchange resin increased the coefficient of its swelling, leaving the structure unchanged. The resin structure was controlled by electron microscopy and X-ray scattering of low angle. When the resin samples with the swelling coefficients of 3.5 and 4.0 were used, 30-40% of proteins, 50% of reducing agents and neutral carbohydrates and a small quantity of phosphorus were adsorbed.

Adsorption↗
PubMedDate not supplied

[The effect of urokinase inhibitors on effective fibrinolytic activity of human urine].

The levels of fibrinolytic activity of human urine as a source of the thrombolytic enzyme urokinase in various volunteers differing in sex and age. The fibrinolytic activity was distinctly altered after mixing of urine obtained from various donor groups as a result of varying quantitative and qualitative contents of urokinase inhibitors in individual volunteers. The specific biological activity of urokinase was studied in the presence of various inhibitors. Prescreening of the initial raw material (urine) should be made to isolate the most effective samples of the thrombolytic enzyme urokinase.

Adolescent↗
PubMedDate not supplied

[Arthrobacter proteinase and its effect on fibrin thrombi].

Arthrobacteria are producers of proteolytic enzymes. Optimal cultivation conditions were determined for Arthrobacter luteus and the mineral composition of the culture medium providing active synthesis of proteinase was optimized. The proteinase was isolated from the culture liquid of Arthrobacter luteus using preparative ion-exchange chromatography on a carboxyl cation exchange resin KMT. The enzyme was homogeneous according to gel chromatography on Sephadex G-200 and had a molecular weight of 24 kD. The fibrinolytic activity of the proteinase is comparable with that of trypsin.

Arthrobacter↗
PubMedDate not supplied

[Catalase inactivation during storage in solution and its stabilization by polysaccharides of microbial origin].

It has been experimentally shown that the rate of inactivation of pure catalase in solution does not obey the kinetics of the first order. The kinetics of denaturation taking into account partial stabilization of catalase due to the formation of intermolecular complexes of native molecules with denatured molecules has been derived. Using the equation, the rate of catalase inativation during storage in pure solutions has been calculated from the experimental data. Polysaccharides of microbial origin can also stabilize catalase. The kinetics of catalase inactivation in the system where the enzyme and polysaccharide forms a reversibly dissociating complex has been described.

Catalase↗