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Biomedical subjects

L Joyce

Publications and source records attributed to L Joyce.

26 records · Page 2Linked to original sources
PubMed1979

[Anti-HBs antibody. A new immunoenzymatic detection. Preliminary results (author's transl)].

The detection of anti-HBs antibody has been carried out using a new technique where a system for the detection of HBs antigen in the solid phase is adopted to demonstrate the presence of anti HBs antibody by inhibition of the immunoenzymatic reaction. The sensitivity of the method has been compared: with that of passive haemagglutination and with that of radioimmunoassay in liquid phase, for human serum obtained from 10 chronic antibody carriers and also serum from 1 rabbit, with that of solid phase radioimmunoassay for 91 positives serums. The method appears: in the range of sensitivity of passive haemagglutination and radioimmunoassay in the liquid phase, less sensitive than that of solid phase radioimmunoassay, far superior to that of electrosyneresis.

Animals↗
PubMed1977

Differential effect of hypophysectomy on the synthesis of beta-glucuronidase and other androgen-inducible enzymes in mouse kidney.

The levels of several androgen responsive enzymes including beta-glucuronidase, alcohol dehydrogenase, D-amino acid oxidase and arginase, were compared in kidneys of normal and hypophysectomized female mice after treatment with testosterone. While hypophysectomy did not alter the basal level of glucuronidase, the androgen-mediated accumulation of kidney beta-glucuronidase was greatly decreased in hypophysectomized mice. Measurements of the rate of synthesis of glucuronidase showed that after androgen treatment the enzyme was synthesized in kidney of hypophysectomized mice at only 5% the normal rate. Glucuronidase activity in seven other organs was not appreciably affected by treatment with androgens or by hypophysectomy. Unlike the effect of hypophysectomy on kidney glucuronidase, there was no reduction in the accumulation of alcohol dehydrogenase or D-amino acid oxidase in kidney of hypophysectomized mice after androgen treatment. Hypophysectomy caused a large reduction in kidney arginase activity. However, subsequent administration of testosterone restored much of this activity. It is concluded that there are at least two mechanisms by which androgens increase enzyme activity in kidney. The normal increase in activity or rate of synthesis of beta-glucuronidase following androgen administration requires pituitary hormones and/or products of these hormones, while the increase in activity of enzymes like alcohol dehydrogenase and D-amino acid oxidase does not require pituitary hormones.

Adrenalectomy↗
PubMedDate not supplied

Detection and measurement of rheumatoid factor using a new immunoenzyme technique with peroxidase/anti-peroxidase complex.

A new, solid-phase enzymatic technique is proposed for the detection and measurement of rheumatoid factors (RF). A complex (PAP) consisting of peroxidase and rabbit IgG anti-peroxidase antibodies was used as antigen. The sensitivity of the technique is such that even "physiological" levels of RF may be detected in the majority of serum samples. The intra-assay and inter-assay repeatability (6-8% and 12%) is better than that for either of the traditional agglutination techniques (latex fixation test and Waaler-Rose reaction, or LWR) and this means that the serological evolution of the illness may be followed more accurately for each patient. The detection of non-agglutinating RF was also possible. The antigen used (i. e. rabbit IgG) was fixed immunologically to the enzyme, hence it was not denatured by chemically labelling. This antigen resembles that of the Waaler-Rose reaction. A variation of this method enabled the detection of RF immunoglobulin class, when only the IgM fraction of the sera tested could be fixed to the solid phase by means of anti-mu F(ab')2 antibody before introduction of the antigen. The affinity constant could be measured without antibody purification. In 211 cases of adult rheumatoid arthritis, abnormally high levels of RF were revealed in 78% of the subjects using the PAP technique and in only 53% with the agglutination (LWR) techniques. On the other hand, the RF levels were within normal limits in the 51 cases of other inflammatory rheumatisms lacking RF according to LWR techniques, in 62 cases of non-inflammatory diseases (hip osteoarthrosis, low back pain) and also in the 42 cases of other inflammatory diseases.

Animals↗
PubMedDate not supplied

One Stop Post Op cardiac surgery recovery--a proven success.

The One Stop Post Op model for open heart surgery recovery is an innovative approach to post op care utilized in only a few facilities in the country. This model calls for an integration of acute ICU and step-down phases of care, thus changing the paradigm for nursing care of the open heart surgery patient. Typically, hospitals incur inefficiencies transferring the patient through multiple levels of care, thus resulting in a "disconnect" as new caregivers relearn the patient's care requirements and special needs. The construction of a "one stop" unit allows the patient to remain stationary while the service level changes to accommodate changing care needs. The cardiac "one stop" model is similar to the LDRP concept for obstetrical care. The One Stop Post Op patient rooms are designed to accommodate every level of patient acuity. All rooms meet the regulations for critical care room design, however this is where the aesthetic similarity ends. The patient environment looks more like hotel rooms rather than the traditional ICU setting. Cabinets designed to cover medical gases, in the room's private bathrooms and comfortable furnishings help to create a patient focused environment conducive to recovery. This model has been utilized by several facilities and has demonstrated clear clinical and economic advantages for patients, families, and health care providers. Implementing an open heart surgery (OHS) program presents the opportunity for several community based hospitals to challenge the way they have been providing patient care and establish an innovative approach to post surgery patient care. The One Stop Post Op cardiovascular recovery unit is designed to receive the OHS patient directly from the operating room and to be the "care unit" for the patient's entire stay. Patient flow, quality monitoring and caregiver acceptance in this unit requires new paradigms from the traditional two or three step post OHS care delivery process. The One Stop Post Op model focuses the delivery of care on the patient. With proven success in clinical outcomes, patient, physician and caregiver satisfaction, it is anticipated that this innovative approach will drive hospitals to integrate clinical process with physical planning in the future.

Critical Care↗