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Biomedical subjects

L Joshi

Publications and source records attributed to L Joshi.

31 records · Page 2Linked to original sources
PubMed1977

Effect of contraceptive steroids on human lactation.

Biochemical studies to detect changes occurring in the composition of mother's milk from the initiation of proper lactation to weaning were done in a group of 36 postpartum women, volunteers who were either using the conventional contraceptives or taking a low-dose combination pill, a low-dose progestational compound, or a 3 or 6 monthly injection as a mode of contraception. Apart from a significant increase in the protein content of milk and a slight increase in the quantity, the 3 monthly injection group behaved exactly like the control. On the other hand, the 6 monthly injection group showed a significant increase in quantity but with a significant decrease in protein, fats, and calcium. The low-dosage progestogens group showed a significant decrease in the quantity of fats and the calcium content of milk, but, surprisingly, the addition of 10 micrograms of estrogens to the low-dosage progestogen indicated a pattern almost similar to the control.

Contraceptive Agents, Female↗
PubMed1974

Conjoined twins.

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Abortion, Spontaneous↗
PubMedDate not supplied

ELISA--its evaluation in diagnosis of tuberculosis meningitis.

Diagnostic utility of an enzyme linked immunosorbent assay (ELISA) in hospitalised patients with clinical symptoms suggestive of tuberculous meningitis (94 cases) was studied. CSF Anti-tubercular IgG antibody levels were estimated by ELISA in 44 cases of proven tuberculous meningitis, 24 proven pyogenic meningitis and in 48 non-tuberculous cases. The cut off dilution was established at a CSF dilution of 1 : 1 (undiluted) with positive and negative controls. The ELISA test had a sensitivity of 97.72% and a specificity of 95.35% using MSE antigen. When compared with conventional smear examination and culture isolation from specimens, the ELISA test proved statistically superior in the detection rate or diagnosis of clinically suspected tuberculous meningitis cases (P less than 0.05).

Antibodies, Bacterial↗
PubMedDate not supplied

Influence of baculovirus-host cell interactions on complex N-linked glycosylation of a recombinant human protein.

The conditions required for mammalian-type complex N-linked glycosylation of human proteins produced in insect cells with the baculovirus expression vector system were investigated. Marked alterations to N-linked glycosylation of human placental secreted alkaline phosphatase (SEAP) were observed with different baculovirus species, insect cell lines, and cell culture media. When a recombinant Autographa californica nucleopolyhedrovirus (AcMNPV) was used to produce SEAP in Trichoplusia ni (Tn-4h) cells cultured in serum-free medium, structural analyses indicated <1% hybrid and no complex oligosaccharides attached to SEAP, a typical result with the baculovirus expression vector system. However, when fetal bovine serum was added to the culture medium, 48 +/- 4% of the oligosaccharides were hybrid or complex (but asialylated) glycans. When a recombinant T. ni nucleopolyhedrovirus (TnSNPV) was similarly used to express SEAP in Tn-4h cells cultured in serum-containing medium, only 24 +/- 3% of the glycans contained terminal N-acetylglucosamine and/or galactose residues. In contrast, SEAP produced in Sf9 cells grown in serum-containing medium with AcMNPV contained <1% hybrid oligosaccharides and no complex oligosaccharides. The results illustrate that baculovirus type, host cell type, and the growth medium all have a strong influence on the glycosylation pathway in insect cells, resulting in significant alterations in structures and relative abundance of N-linked glycoforms. Although the addition of sialic acid residues to the SEAP glycans was not detected, possible approaches to obtain sialylated glycans are discussed.

Alkaline Phosphatase↗
PubMedDate not supplied

Production of a sialylated N-linked glycoprotein in insect cells.

Under High Aspect Ratio Vessel (HARV) bioreactor culture conditions designed to simulate the microgravity of orbital space flight, insect tissue culture cells infected with a baculovirus expression vector produced a human glycoprotein with tri- and tetra-antennary complex N-linked oligosaccharides containing terminal sialic acid residues. The oligosaccharide structures were similar to those produced in human placental cells. Insect cells cultured in T-flasks only performed incomplete oligosaccharide processing. The mechanism of HARV-mediated changes in the eukaryotic N-linked glycosylation pathway was investigated and could be mimicked under T-flask growth conditions with the addition of N-acetylmannosamine to the culture medium. The significance of these investigations is discussed with respect to the production of recombinant therapeutic glycoproteins, insect physiology, and orbital space flight.

Animals↗