Search PubMed⌕ Search

Biomedical subjects

L Jacques

Publications and source records attributed to L Jacques.

At least 55 records · Page 3Linked to original sources
PubMed1978

A factor from neurons increases the number of acetylcholine receptor aggregates on cultured muscle cells.

There is an increase in the number of acetylcholine (AcCho) receptor aggregates on striated embryonic mouse myotubules when they are cocultured with clonal neuroblastoma-glioma hybrid cells. Medium conditioned by hybrid cells contains a factor which increases the number of AcCho receptor aggregates on myotubes cultured from mouse, rat or chick muscle. AcCho receptor-aggregating activity was present in medium conditioned by the neuroblastoma parent clone but was not detected in medium conditioned by cells of the parent glioma clone, fibroblasts, or HeLa cells. The factor increased the aggregation of AcCho receptors within 24 hr without a significant increase in the total number of AcCho receptors, and its action did not depend on myotube protein synthesis. The factor appears to rearrange the distribution of myotube AcCho receptors either by aggregating mobile AcCho receptors or by stabilizing labile receptor aggregates.

Cells, Cultured↗
PubMed1978

[Variations in the phagocytic potency of circulating leukocytes from rats which received a thermal injury. Effects of an immunostimulation with Corynebacterium granulosum (author's transl)].

Experiments were carried out to study in vitro the variations of phagocytic activity of circulating leukocytes from rats which received a thermal injury and were either immunostimulated or not by a single iv injection of C. granulosum. The thermal injury which was applied at about 10 percent of the body surface was of an intermediary type ; it developed towards necrosis before cicatrising on about the 30th day. Phagocytic activity was expressed as the percentage of "active" phagocytes or of those cells which phagocytosed Sta. aureus and S. typhi murium. In not immunostimulated rats, the phagocytic activity was profoundly depressed as a result of the thermal injury ; this was most marked at about 8 days after the thermal injury (close to 38% compared with 80% in control-not-burned-rats). The phagocytic potency or circulating leukocytes from burned and immunostimulated rats corresponded at about the 8th day to that of healthy rats. The reported variations are statistically significant, as assessed by the Student "t" and X2 tests. Our study showed that those rats which were burned in our experimental conditions responded favourably to the immunostimulation. It would be interesting to check whether the use of anaerobic corynebacteria in man could represent an adjunct treatment of those septic complications which are so frequent in severe burns.

Animals↗
PubMed1977

[Serum and urinary antibodies in pyelonephritis (author's transl)].

Pyelonephritis is accompanied by complex immunological reactions, the study of which is just now being undertaken. Study of 216 patients with a significant monomicrobial urinary infection showed that in more than 95 per cent of cases of Gram negative infection the urine contained IgG type antibodies, often associated with IgA. These antibodies may be easily detected using an immunofluorescent technique, which would appear to be the best method available at present for distinguishing between pyelonephritis and an infection of the lower urinary tract. They may also be found in the serum, though less frequently. Detection by immunofluorescence is markedly more sensitive than using the classical passive haemagglutination technique. The biological significance of these antibodies remains imprecise, and their protective or facilitating role may be discussed. Their specificity must be determined, in order that sequential study of these immunological reactions may be of help in the conduct of treatment.

Antibodies, Bacterial↗
PubMed1977

Antibodies in Escherichia coli urinary tract infection.

In 105 adults with E.Coli urinary tract infections, IgG coated bacteriuria was found in 8/9 with acute pyelonephritis (PN), 17/20 with chronic PN, and in only 2/76 with lower UTI. IgA was present in 66% of PN, but IgA secretory piece in less than 10%. These urinary IgG antibodies were, at least in part, synthesised in the kidney because serum IgG antibodies were detected by indirect immunofluorescence in only half the patients. 06, 018, 022 E.Coli serotypes were the three most frequently found O groups, but their prevalence in PN is not significant. The immunology of urinary tract infection (UTI) is still a subject of little interest in adult nephrology; but antibody production is a well-characterised event in pyelonephritis (PN), the study of which seems to be the best indirect procedure for localising the site of UTI. We have analysed the production of urinary and humoral antibodies, and their correlation with E.Coli serotypes in patients with E.Coli UTI.

Adult↗
PubMed1975

[Immunofluorescence study of bacteriuria. A new technic for the localization of urinary infection].

The detection by immunofluorescence of immunoglobulins fixed to the wall of urinary bacteria makes it possible to localise the site of the urinary infection. The presence of immunoglobulins was noted in 18 out of 19 patients with a high urinary tract infection involving the renal parenchyma. They were absent in 25 out of 28 subjects with a lower urinary tract infection. The three apparently discordant positive reaction involved 1 case of prostatitis and two cases in which the diagnosis of pyelonephritis was made later. In the case of renal involvement, these immunoglobulins, essentially IgG, are seen with greater frequency than humoral antibodies. Whilst the biological significance of these immunoglobulins remains uncertain, their existence probably being a reflection of parenchymatous inflammation, this nevertheless is a new and, apparently, reliable, method for the determination of the site of an infective process.

Adult↗
PubMedDate not supplied

[Urinary Escherichia coli infections in the rat. Significance of the study of antibody-coated bacteria].

The value of antibody coated bacteria (ACB) determinations in localizing urinary tract infections has yet to be confirmed. According, a study of ACB was carried out in rats in which either an upper or lower urinary tract infection has been experimentally induced with E. coli. Several ACB determinations were made for each animal prior to being sacrificed for pathological studies on the 7th day after inoculation. ACB were found only in those animals with parenchymatous lesions of the kidney or prostate, thus supporting the localizing value of this technique.

Animals↗