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Biomedical subjects

L J Wright

Publications and source records attributed to L J Wright.

At least 37 records · Page 2Linked to original sources
PubMed1981

Monoclonal antibodies to blood group antigens on ruminant red cells.

Hybridomas were made between NSI/1-Ag-4-1 mouse myeloma cells and spleen cells Balb/c mice immunized with red cells from sheep (SRBC), goats (GRBC) and cattle (CRBC). The monoclonal antibodies thus produced were tested against a panel of RBC from 80 sheep, 20 goats and 100 cattle of known blood types. One antibody reacted with all RBC from all 3 species, two with all GRBC and SRBC but not CRBC, one was specific for SRBC and two others for CRBC. In addition two monoclonal antibodies were reactive in the B blood group system and two in the FV system of cattle. IgM and IgG concentration in ascite fluids reached up to 8 mg/ml. It is concluded that the hybridoma technique has considerable potential for the production of blood-typing reagents.

Animals↗
PubMed1981

Culture of sheep X mouse hybridoma cells in vitro.

Hybridomas were made between NS1/1-Ag4-1 mouse myeloma cells and spleen and lymph node cells from a sheep immunized with sheep red cells (RBC). The hybrid colonies grew well in culture but there was a substantial loss of sheep chromosomes. No hemolytic or agglutinating antibodies were detected in the culture supernatants after the 17th day following fusion, but immunofluorescence tests indicated that a few of the cells may have been expressing sheep IgG. Cytogenetic comparison of cells grown with and without HAT medium provided evidence that the enzyme HGPRT is located on the X chromosome of sheep as it is in man and mouse. Hybridoma isozyme patterns of esterase, G6PD, 6PGD, NP, LDH and SOD tested between the 63rd and 71st day of culture were like those of NS1; NP and LDH also showed zones that probably came from the sheep component.

Animals↗
PubMed1980

Hybridization between T and B lymphoma cell lines.

The AKR thymoma line BW 5147 has been successfully hybridized with the IgM-bearing (BALB/c x NZB)F1 B lymphoma line WEHI 231. In the hybrids formed, T-cell characteristics were dominant, i.e. there was no expression of IgM but continued expression of Thy-1.1 in eight out of eight lines. Moreover, in two out of eight lines, the Thy-1.2 allele was also expressed. We conclude that, in its ability to hybridize, BW 5147 is not restricted to cells of similar ontogenetic origin (i.e. T cells) and that fusion with the thymoma can lead to suppression of B-cell gene expression and derepression of genes for T-cell markers.

Alleles↗
PubMed1975

Spontaneous lesions in the aorta of the common brown hare (Lepus europaeus).

The intimal surface of the aortic arch of 16 of 20 male common brown hares and 10 of 14 females had raised pale yellow to grey-white plaques up to 6 mm in diameter. These lesions were characterised by necrosis and calcification mainly in the tunica media and were not related to the age of the hares. In the hare this vascular disease may develop after injury to the connective tissue during obliteration of the ductus arteriosus. Although these aortic lesions were not the 'spontaneous' atherosclerotic variety they were morphologically similar to those in rabbits.

Animals↗