Biomedical subjects
Publications and source records attributed to L J COLE.
From a mixture of tobacco mosaic virus pentosenucleic acid (RNA) and calf thymus deoxypentosenucleic acid (DNA) (2 mg each), 73 percent of the RNA was separated free of DNA by discontinuous elution chromatography with phosphate buffers at pH 6.7 on columns of calcium phosphate.
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The bactericidal substance present in mitochondrial extracts from rat leucocytes was found to be non-dialyzable, fractionated at 30 per cent (NH(4)SO(4) saturation and was able to withstand temperatures up to 100 degrees C. The tentative identification of a lipoprotein as the responsible bactericidal substance is based on various observations; an ultraviolet absorption at a peak associated with alpha, beta unsaturated fatty acids, 205 to 208 mmicro, inactivation of the bactericidal substance by ether treatment at -70 degrees C. but not at 0 degrees C. and inhibition of the bactericidal activity with lipase treatment as well as with trypsin.