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Biomedical subjects

L J Alexander

Publications and source records attributed to L J Alexander.

At least 19 recordsLinked to original sources
PubMed1992

The sequence of a porcine cDNA encoding alpha-lactalbumin.

A cDNA clone encoding porcine alpha-lactalbumin (alpha LA) was isolated and sequenced. The longest clone was 688 nucleotides (nt) long and encoded a preprotein of 141 amino acids (aa) including a leader peptide of 19 aa. The porcine cDNA exhibited a nt similarity of between 72.2%-83.5% to other alpha LA cDNAs and an aa similarity of between 50.8%-85.2% with other alpha LA aa sequences. The derived aa sequence varied at three positions from a previously reported sequence for porcine alpha LA obtained by direct aa sequencing.

Amino Acid Sequence↗
PubMed1992

Cloning and sequencing of the porcine lactoferrin cDNA.

cDNA clones encoding the entire porcine lactoferrin protein were isolated and sequenced. The porcine lactoferrin cDNA sequence presented here is 2259bp in length and encodes a leader peptide of 19 amino acids and a mature protein of 684 amino acids. Comparisons with other lactoferrins indicate a single glycosylation site. The iron- and anion-binding sites, and the cysteine residues involved in disulphide bonds, are conserved between the lactoferrin proteins.

Amino Acid Sequence↗
PubMed1992

Sequence of porcine beta-lactoglobulin cDNA.

cDNAs encoding porcine beta-lactoglobulin were isolated and sequenced. The porcine beta-lactoglobulin cDNA is 768bp in length and encodes a pre-protein of 178 amino acids. One additional cDNA clone was found to encode an additional amino acid (lysine) in the mature protein.

Amino Acid Sequence↗
PubMed1992

The sequence of porcine alpha s1-casein cDNA: evidence for protein variants generated by altered RNA splicing.

A cDNA library was constructed from mRNA isolated from lactating porcine mammary gland and screened with a bovine alpha s1-casein cDNA clone. Three classes of cDNA isolated varied in the number of bases within the coding region. The full length porcine alpha s1-casein cDNA is 1124bp and codes a preprotein of 206 amino acids. The other two classes of alpha s1-casein cDNA lacked 18bp and 60bp respectively when compared to the 1124-bp cDNA sequence. PCR amplification confirmed the presence of these sequences in total RNA. These differences appear to be due to altered RNA splicing.

Amino Acid Sequence↗
PubMed1992

The sequence of porcine beta-casein cDNA.

Porcine cDNAs clones encoding beta-casein were isolated and sequenced. The porcine beta-casein cDNA is 1100bp in length, excluding the poly(A) tail, and encodes a preprotein of 232 amino acids.

Amino Acid Sequence↗
PubMed1992

Ocular vitamin therapy. A review and assessment.

Vitamin therapy for diseased conditions of the eye has regained momentum in the United States, especially for the treatment of age-related macular degeneration. This review looks at the potential effects of vitamin therapy as it relates to everyday primary eye care practice. The discussion covers the generalities of vitamins, their actions, and potential toxicities. In addition, specific attention is paid to the ocular conditions that have been proposed as benefiting from oral and topical vitamin therapies. The analysis is made on a clinicopathological basis rather than being drawn from anecdotal accounts and unsubstantiated claims.

Eye Diseases↗
PubMed1992

The sequence of porcine alpha s2-casein cDNA.

cDNA clones encoding the entire porcine alpha s2-casein message were isolated and sequenced. The porcine alpha s2-casein cDNA is 1093 bp, excluding the poly(A) tail, in length and encodes a preprotein of 235 amino acids.

Amino Acid Sequence↗
PubMed1992

Variations in physician response to consultation requests for Hollenhorst plaques: a pilot study.

This is a report on physicians' response to a pilot survey regarding the method of management of a patient sent to them for a health evaluation because of a Hollenhorst plaque detected during a routine eye examination. Several specialties and sub-specialties were surveyed. The results demonstrate a wide variability in the methods chosen for management of the patient, indicating the need to consider standardizing the health evaluation. A review of literature suggests that the patient with a Hollenhorst plaque is at increased risk for both cardiovascular and cerebrovascular disease and appropriate measures should be taken to minimize the risk. The pilot survey suggests that more in-depth surveys are necessary to establish standards of care for the patient with Hollenhorst plaques as well as other ocular conditions related to systemic disorders.

Arteriosclerosis↗
PubMed1992

Stroke.

Stroke is a primary cause of death and debilitation in the United States. There is both a geographical and race distribution throughout the country. A major health initiative over the next few years is to further reduce the incidence of stroke, especially in the STROKE BELT. There are several "eye signs" of impending stroke including transient monocular blindness, retinal vascular occlusive diseases, ischemic retinal syndromes, visual field defects and retinal emboli. Optometrists can serve as a first line of defense in the death and debilitation from stroke by the recognition of the "eye signs" associated with the most common variant of stroke--the thromboembolism. The primary care provider must also provide recommendations regarding the proper consultation to avert a total stroke.

Cerebrovascular Disorders↗
PubMed1991

The secondary glaucomas.

Glaucoma resulting from secondary congenital, ocular, or systemic conditions represents a formidable diagnostic and management challenge for optometrists. This paper discusses the pathogenesis, diagnosis, and treatment of six common forms of the disease: exfoliation syndrome, pigmentary glaucoma, neovascular glaucoma, lens-induced glaucoma, glaucoma accompanied by ocular inflammation, and trauma-induced glaucoma. For each of these conditions, clinical experience, observational skills, and an understanding of the mechanisms by which elevated intraocular pressure may result are prerequisites for accurate diagnosis. Management requires that the intraocular pressure be reduced and that the underlying condition be diagnosed so that other appropriate treatment may be initiated. Follow-up is predicated on risk factors, relative control of the intraocular pressure, and the fragility of the optic nerve.

Adrenergic beta-Antagonists↗
PubMed1991

Low-tension glaucoma.

Explore the source record for details and available documents.

Adrenergic beta-Antagonists↗
PubMed1991

Diagnosis and management of primary open-angle glaucoma.

Primary open-angle glaucoma offers a significant diagnostic and management challenge for primary care providers. Uncertainty clouds the definition of the disease, its prevalence, the sensitivity and accuracy of the clinical tests used for its diagnosis, the efficacy of antiglaucoma medications, the compliance of patients with these drug regimens, the appropriate use of follow-up examinations, and the use of surgical techniques in lieu of medical management. This discussion addresses these many vexing issues for the purpose of assisting primary care providers to better diagnose and manage glaucoma patients.

Fundus Oculi↗
PubMed1978

Automated perimetry.

The history, theory, and practical application of automated perimetry are discussed. Optometrists are urged to consider increasing the efficiency of their examination procedures by using automated field testing--in particular, automated perimetry.

Humans↗