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Biomedical subjects

L I Vaneeva

Publications and source records attributed to L I Vaneeva.

29 records · Page 2Linked to original sources
PubMed1978

[Specific and general antigenic determinants of flagellin of certain Salmonellae].

As known, in typing of salmonellae H-antigens play an important role. Flagellae bear on their surface specific antigenic determinants, this permitting to differentiate H-antigens serologically. Monomeric form of H-antigens d,a,b,1,2 bear on their surface not only specific, but also common for the given H-antigens determinant group. Common flagellin determinant group in the flagella is screened.

Antigens, Bacterial↗
PubMedDate not supplied

[The comparative characteristics of the peroxidase conjugates used in Soviet immunoenzyme test systems for detecting HIV antibodies].

Five lots of peroxidase conjugates were studied in enzyme immunoassay systems "Recombinant-HIV" and "Peptoscreen-2" for detection of antibody to HIV. The conjugates differed from each other by the source of generation and methods of preparation. The conjugates were studied in biochemical tests and ELISA. When panels of sera from HIV-infected children and adults were employed, the advantages of using the anti-immunoglobulin conjugate over protein A-peroxidase conjugate were demonstrated, as the former increased the sensitivity of the test systems to HIV antibody detection.

Evaluation Studies as Topic↗
PubMedDate not supplied

[The problems of a loss of activity of the sorbed ligands in heterogeneous immunoenzyme analysis].

Literature containing a number of data on the problem of the loss of activity of adsorbed ligands in the heterogeneous enzyme immunoassay has been analyzed. This analysis has demonstrated that this process is the superposition of the processes of both denaturation and oxidation of the adsorbed ligand, as well as its desorption from the surface of the plate. The proofs of both denaturation and desorption increments of the process of activity loss are presented. Attempts at the theoretical calculations of desorption components with the use of the concept and parameters of the solubility of plastic and dipeptides have been successfully made. Some ways for decreasing the loss of activity of solid-phase antigens (antibodies) have been outlined.

Adsorption↗
PubMedDate not supplied

[The use of the physicochemical laws governing the sorption properties of antigens (antibodies) in relation to the plastic solid phase for enhancing the sensitivity of immunoenzyme analysis test systems in the diagnosis of infections].

Some practical aspects of the theory of adsorption of bioligands on plastic surfaces have been considered and discussed. Modifications of experimental and actual antigens (antibodies) have been theoretically and practically realized on the concept of "solubility parameters" by means of pin-point hydrophobization. The use of this method has been shown to increase the sensitivity of individual ELISA systems based on rabbit and mouse immunoglobulins 1.5- to 2-fold; the sensitivity of the assay system for the evaluation of HBsAg increases 8- to 10-fold. Theoretical calculations making it possible to map out the ways to further increase the sensitivity of different ELISA systems are given.

Adsorption↗
PubMedDate not supplied

[The use of modified polystyrene plates in solid-phase immunoenzyme analysis for the diagnosis of infections].

The practical aspects of the theory of the adsorption of bioligands on the plastic surface have been considered and discussed. The possibility of standardizing polystyrene plates, as well as obtaining plates with the predetermined level of adsorption activity with respect to given antigens (antibodies), has been shown with the use of the patented technology for the modification of polystyrene plates. The data on the adsorption of antigens used as an experimental model (human and rabbit immunoglobulin fractions) are presented, and an increase in adsorption activity has been shown with the use of modified plates. For practical confirmation, some examples of using these plates in actual assay systems intended for the evaluation of HBsAg carriership and antibodies to HIV are given. A considerable increase (2- to 10-fold) of the signal from positive samples has been shown to occur, the signal from negative samples remaining practically unchanged.

Adsorption↗