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Biomedical subjects

L Hunt

Publications and source records attributed to L Hunt.

At least 127 records · Page 7Linked to original sources
PubMed1978

Subcellular localization of isocitrate lyase in nongreen tissue culture cells.

Density gradient centrifugation and electron microscopy were used to establish that isocitrate lyase present in Rosa cv. Paul's Scarlet cells was located in the mitochondria and not other membrane fractions. The enzyme may be important in glycine and serine synthesis. A comparison between the enzymic activity of isocitrate lyase and the amount of glycine and serine synthesized during logarithmic growth indicated that the activity was great enough to account for all of the carbon entering these amino acids during that stage of growth.

Journal Article↗
PubMed1976

Estimated Drainage of Carbon from the Tricarboxylic Acid Cycle for Protein Synthesis in Suspension Cultures of Paul's Scarlet Rose Cells.

The amount of carbon (mumoles of carbon atoms) drained from the tricarboxylic acid cycle for protein synthesis was compared with mumoles of CO(2) released from the cycle at 2-day intervals during the growth of suspension cultures of Paul's Scarlet rose. We concluded that during the period of most rapid protein synthesis (day 0-4) one-sixth as much carbon was drained from the tricarboxylic acid cycle for protein synthesis as was released as CO(2). By day 8, one-thirtieth of the amount of carbon released as CO(2) was incorporated into protein. Net protein synthesis stopped on day 8, but the evolution of CO(2)/culture continued at its maximum rate until day 10.Similar ratios were calculated based on the recovery of (14)C in protein versus CO(2) following a 3-hr provision of labeled substrates to 3-day-old cells (age of maximum protein synthesis). Provision of acetate-1-(14)C and acetate-2-(14)C indicated from one-eighth to an equal amount of carbon was incorporated into protein as was released as CO(2). When (14)C-labeled intermediates of the tricarboxylic acid cycle were provided, the ratio of (14)C incorporated into protein versus that evolved in CO(2) ranged from 1/0.9 to 1/4.9.Following a critical analysis of the methods used, it was concluded that during periods of rapid protein synthesis, a conservative estimate of the amount of carbon drained from the tricarboxylic acid cycle for protein synthesis was one-fourth of the amount evolved as CO(2) from the cycle.

Journal Article↗
PubMedDate not supplied

Serum lipoprotein(a) in patients heterozygous for familial hypercholesterolemia, their relatives, and unrelated control populations.

Serum lipoprotein(a) (Lp[a]) levels were significantly higher in 89 patients with heterozygous familial hypercholesterolemia (FH) (geometric mean, 22.7 mg/dl) than in 109 normocholesterolemic controls (10.0 mg/dl, p less than 0.05) and 40 controls (9.1 mg/dl, p less than 0.05) with similarly elevated low density lipoprotein cholesterol levels due to other primary hypercholesterolemias. To provide further evidence that the increased serum Lp(a) concentration was due to inheritance of the FH gene, 24 unaffected first-degree relatives were compared with their FH probands. Serum Lp(a) in affected individuals was significantly greater than in unaffected relatives (geometric means, 26.5 versus 13.7 mg/dl, respectively; p less than 0.05). Family membership exerted an effect on serum Lp(a) concentrations, indicating that other genetic influences were also operating, as is known to be the case in general populations. Serum Lp(a) in 30 of the FH patients, who had coronary heart disease, was not significantly different from 30 age- and sex-matched controls with FH but with coronary heart disease (geometric means, 23.6 versus 24.7 mg/dl, respectively). FH is associated with an increase in serum Lp(a). Elevated serum Lp(a) concentrations should probably now be regarded as a component of the clinical syndrome of FH. However, within our FH population Lp(a) did not distinguish those with clinically overt coronary heart disease from those without the disease.

Adult↗
PubMedDate not supplied

Unrelated donor bone marrow transplants in children.

Only a small proportion of children who might benefit from bone marrow transplant (BMT) have an HLA-identical sibling. To provide this potentially curative therapy to patients without a matched related donor, marrow transplants using less well matched related donors or unrelated donors (identified through computerized donor registries) have been performed. We report the outcome of 24 consecutive unrelated donor BMT's performed on children. Eligible diagnosis included acute leukemia (AL) (n = 15), chronic myelogenous leukemia (CML) (n = 4), myelodysplastic syndrome (MDS) (n = 3), and severe aplastic anemia (SAA) (n = 2). All donor/recipient pairs were sero-matched at 5 or 6 of the 6 HLA A, B, and DR antigens. Several different preparative regimens were used, but fractionated total body irradiation (TBI) was used in 20 patients. All recipients received graft-versus-host-disease (GVHD) prophylaxis with cyclosporine-A (CSA), four with short course methotrexate (MTX), 14 in combination with short course MTX and methylprednisolone (MPS), and five in combination with a mouse monoclonal antibody to CD5, coupled to the A-chain of ricin (Xomazyme-65). One patient received CSA and MPS alone after a T-cell depleted marrow transplant. Twenty of 23 evaluable recipients engrafted (87%). Two patients with CML never engrafted and had autologous marrow recovery, one patient with SAA died at 128 days without evidence of engraftment, and there was one early death at day + 9. Fourteen of 20 patients (70%) with stable donor-derived hematopoiesis developed significant acute GVHD > or = grade II). Eleven of 15 engrafted patients who survived > 100 days after BMT developed chronic GVHD (73%).(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMedDate not supplied

Development of a meaningful learning environment in theatres.

During the 1990s, geographical distancing of clinical areas and nurse education establishments led to breakdowns in communication. This article focuses on how Good Hope Hospital NHS Trust and the University of Central England in Birmingham created a meaningful learning environment for student nurses in the operating theatre using a revised version of Crofts and Taylor's (1996) perioperative placement model. The student evaluation has demonstrated that the quality of placement, teaching and supervision were of a high standard and students felt that their nursing skills had been enriched as a direct result of the placement. This article will also examine the background to student placements in the operating theatre.

Attitude of Health Personnel↗