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Biomedical subjects

L Huang

Publications and source records attributed to L Huang.

At least 289 records · Page 16Linked to original sources
PubMed1999

[The level of expression of adhesion molecules CD44v6 and E-cadherin in colorectal cancer and analysis of correlates with metastasis].

OBJECTIVE: To determine the expression of adhesion molecules CD44v6 and E cadherin in colorectal cancer and its relation with metastasis. METHODS: The expression of CD44v6 and E-cadherin in 32 patients with colorectal carcinoma was investigated using immunohistochemical staining, which was performed on surgical specimens. RESULTS: No significant correlation with CD44v6 and histologic type, TNM and Dukes staging was noted, but it was significantly correlated with lymph node and hematogenous metastasis. E-cadherin was strongly expressed in normal epithelium examined. However, E-cadherin expression in colorectal carcinoma was reduced (positive rate 46.9%). Metastatic colorectal carcinoma showed significant reduction (positive rate 20%). CONCLUSION: CD44v6, E-cadherin immunoreactivity are significantly correlated with lymph node and hematogenous metastasis.

Cadherins↗
PubMed1999

[Relationship between the prognosis of retinal detachment in emmetropic eyes and vitreous changes].

OBJECTIVE: To study the relationship between surgical prognosis of retinal detachment (RD) in emmetropic or myopic eyes and vitreous changes. METHODS: Postoperatively, 1,294 eyes of 1,262 patients with rhegmatogenous RD including 243 emmetropic and 1,031 myopic eyes were retrospectively studied. The affects of proliferative vitreoretinopathy (PVR), posterior vitreous detachment (PVD), vitreous liquefaction and concentration on the surgical prognosis were evaluated. RESULTS: The cure rate in emmetropic group (88.5%) was lower than that in myopic group (93.8%, P < 0.01). Also, the cure rate by initial surgery in emmetropic group (65.4%) was lower than that in myopic group (76.4%, P < 0.01). In addition, the occurrences of PVD (39.6%) and liquefaction (76.1%) in emmetropic group were lower than that in myopic group (51.7%, and 81.8% respectively, P < 0.05). However, focal vitreous concentration in emmetropic group (56.8%) was much higher than that in myopic group (19.6%, P < 0.01). CONCLUSIONS: The surgical prognosis of RD especially by initial operation is worse in emmetropic group than that in myopic group. The lower occurrences of PVD and liquefaction but higher occurrence of focal vitreous concentration in emmetropic eyes may have impact on the prognosis. It is possible that the vitreous characteristics in emmetropic eyes with RD have relationship to the occurrence and development of PVR that has the influence on the prognosis to some extent.

Adolescent↗
PubMed1999

[Primary study of transfusion transmitted virus (TTV) infection in the hepatitis patients and blood donors in Hunan].

To investigate transfusion transmitted virus (TTV) infection in the hepatitis patients and blood donors in Hunan, we used nested polymerase chain reaction (nPCR) assay with primers from TTV genome to detect TTV DNA in seventy clinical hepatitis cases and forty-three blood donors. The results were as follows: TTV DNA was positive in ten of thirty eight patients with hepatitis B(26.3%), and twelve of thirty-two patients with non-A through non-E(37.5%). Elevated transaminase (ALT) was observed in those cases (452 U.L-1 and 582 U.L-1, respectively). On the other hand, TTV DNA was positive in four of forty-three normal blood donors. The data indicate that infectious rates of TTV in hepatitis patients and blood donors in Hunan are similar to those in Japan, and TTV's pathogenicity needs further study.

Blood Donors↗
PubMed1999

[RAPD analysis of germplasm in "baizhi"].

OBJECTIVE: To analyse the germplasm in Baizhi. METHOD: Using 12 random primers, over seventeen individuals' RAPD were detected in four populations from wild Baizhi (Angelica dahurica), Qi Baizhi and Hang Baizhi. RESULTS: A total of 40 loci were amplified, 26 of which were polymorphism(65%). The PPS values of Hang Baizhi and Qi Baizhi indicate their low levels of genetic diversity. According to the RAPDinstance analysis, the genetic distance(0.16) between Qi Baizhi and Hang Baizhi is shorter than that of the individuals of the populations of Qi Baizhi and Hang Baizhi, and all of these genetic distances are shorter than those between wild Baizhi and Hang Baizhi(Qi Baizhi). CONCLUSION: Hang Baizhi- and Qi Baizhi belong to the same taxon, which is different from the wild Baizhi.

Angelica↗
PubMed1999

[Antihypertensive study of xingnao qingxuan recipe].

OBJECTIVE: To observe the effect of decreasing blood pressure of Xingnao Qingxuan Casuples. METHOD: The blood pressure in normal and anesthetised cats was examined with direct method, the blood pressure in the conscious SHR was obtained by indirect method. RESULTS: Duodenuos administration(id) of XQC of 2.8 g/kg produced a decrease of blood pressure in normal cats; XQC of 1.4, 2.8 and 5.6 g/kg once a day for 14 days produced a dose-dependent reduction of blood pressure in SHR, 3-4 days after administration the blood pressure returned to the level before but the change was not significant. With gastric administration of XQC of 2.8 and 5.6 g/kg for 3 times, the incubation period of eyeball tremor induced by chloroform by dropping into the ear was prolonged by 14.4% and 13.0%, and the keeping time shortened by about 33.3% and 23.3% respectively. Brain basic arterial spasm induced by KCl or 5-HT in dog was relaxed obviously by XQC in vitro experiment. CONCLUSION: XQC has the effect of reducing blood pressure resisting dizziness, which is related to the diastole of brain blood vessel.

Animals↗
PubMed1999

[Morphological studies on 18 species of gualoupi].

OBJECTIVE: To gain a clear idea of the drug Gualoupi and its adulterants. METHOD: Collecting data and spot investigating in 30 provinces, cities and autonomous regions in China. RESULT: Gualoupi used as drug in China consists of 15 species of Trichosanthes, 1 species of Momordica, 1 species of Citrullus and 1 species of Adenia(Passifloraceae). CONCLUSION: The main commodity is the rind of T. kirilowii or T. rosthornii, and the main adulterant is the rind of T. hupehensis.

Drug Contamination↗
PubMed1999

[Skeletal effect of xianzhen gubao on preventing prednisone-induced osteoporosis in male rats].

OBJECTIVE: To study the effect of Chinese medicine Xianzhengubao (XZGB) capsules of compound prescription on corticosteroid-induced osteoporosis in rats. METHOD: 3-month-old SD male rats were randomly divided into basal control and aging control, prednisone (4.5 mg.kg-1, ig, twice a week) and XZGB capsules were used in three different doses (0.2, 0.4, 0.8 g.kg-1.d-1 ig)6 times a week. All the rats were killed. On the 90th day afteradministration. The proximal tibiae were processed in undecalcified sections at a thickness of 4-10 microns for histomorphometric analysis. RESULT: Compared with the aging controls corticosteroid reduced markedly the trabecular bone mass due to increase of bone resorption and decrease of bone formation (%Tb. Ar 52.9%). Treatment with XZGB capsules in high doses increased significantly the trabecular area as compared with the hormone control(% Tb. Ar + 91.2%). The protective mechanism of XZGB capsules mainly consists in suppressing bone resorption and mildly increasing bone formation in rats. CONCLUSION: Xianzhen gubao capsules have an effect on preventing bone loss in rats induced by corticosteroid, and higher doses produce greater effect.

Animals↗
PubMed1999

[Analysis of the genetic relationship among the three types of Trichosanthes kirilowii Maxim. by random amplified polymorphic DNA (RAPD)].

OBJECTIVE: Discussing the discordance in purchasing and utilizing a new type of Trichosanthes kirilowii called "Niuxin-gualou" from Shandong Province. METHOD: The genetic relationship among the three types of T. kirilowii including "Ren-gualou", "Tang-gualou" and "Niuxin-gualou" from Shandong was analyzed by random amplified polymorphic DNA(RAPD). RESULTS: Among the three types studied, the genetic distance is the smallest between "Niuxin-gualou" and "Ren-gualou", and the largest between "Niuxin-gualou" and "Tang-gualou". CONCLUSION: "Niuxingualou" is genetically more similar to "Ren-gualou".

DNA, Plant↗
PubMed1999

[Histological studies on rinds of 18 species of Gualou].

OBJECTIVE: To make clear the histological characteristics of rinds for eighteen species of Gualou. METHOD: Observing the cross sections and separate divide of the rinds with light microscope. RESULT AND CONCLUSION: Of eighteen rinds of Gualou, each trichome on exocarp, wood fiber and thickness of ring of stone cell are different from one another, this distinction can be used to identify the different rinds of Gualou.

Citrullus↗
PubMed1999

[An approach to some problems on utilization of medicinal plant resource in China].

OBJECTIVE: To provide some reference for the research on the utilization of medicinal plant resources. METHOD: Based on a review of the achievements in utilizing medicinal plant resources, three aspects were discussed: the utilization and conservation of the endangered and rare medicinal plants; the research on the authenticity and superiority of medicinal plants; and the quality control and evaluation of medicinal plants. RESULTS: Although great achievements have been made, some problems still exist. CONCLUSION: The research on the development and utilization of medicinal plant resources has broad prospects.

Conservation of Natural Resources↗
PubMed1999

[Agrobacterium tumefaciens mediated maize transformation].

Several maize inbreds were transformed with Agrobacterium tumefaciens EHA101 (pGIH). Transgenic maize plants were obtained. Frequency of transformation of maize inbred Suyu No. 1 can reach 8.1%. Results of PCR and Southern blot analysis proved that T-DNA was stably integrated into the genome of maize. Staining with X-gluc confirmed the expression of GUS gene in maize cells. The band amplified by inverse PCR showed that the copy number of transgene in three transformants was single. After long term of subculture, some hygromycin resistant calli lost their regeneration ability. Although Southern blot probed the integration of gusA gene in their genome, GUS activity cannot be detected in those calli. Southern blot analysis of HpaII digest DNA showed that transgenic gusA gene was highly methylated.

Agrobacterium tumefaciens↗
PubMed1999

[Changes in rat erythrocyte hemorheology caused by morphine dependence].

This study was aimed at the effect of morphine on the biomechanics properties of rat erythrocyte membrane in vivo and in vitro. The shear elastic modulus (E) and the surface viscotsity viscosity (micron) of rat erythrocyte membrane was measured by using the method of the new ektacytometry. The changes in hemorheology caused by morphine dependence were investigated and the mechanisms of the changes are suggested.

Animals↗
PubMed1999

[Spectral properties of broad emission bands of Cr3+ in A3M2Ge3O12 (A = Cd2+, Ca2+; M = AI3+, Ga3+, Sc3+) garnets].

The spectral properties of the Cr3+ broad emission band in A3M2Ge3O2 : Cr(A = Cd2+ ,Ca3+; M = Al3+,Ga3+,Sc3+) germanate garnets at room temperature are reported for the first time. In the order of Al3+ --> Ga3+ --> Sc3+ and Cd2+ --> C2z+ ions which occupy the octahedral and duodeahedral sites, respectively, R-NIR broad emission band of 4T2 --> 4A2 level transition, emission peak, and the spectral long wavelength and short wavelength sides of Cr3+ shift to long wavelengths having lower energy. The reason is that the crystal field strengths in the octahedral decrease, while the cation ionic radii in the A3M2Ge3O12 system garnets increase gradually. The R-NIR emission intensity of Cr3+ is increased with some Sc3+ ions substitution for Al3+ or Ga3+ ions in octahedral sites.

English Abstract↗
PubMed1999

[Luminescence character of Ce3+ in rare earth borate glasses].

Ce3+ doped rare earth borate glasses (BLBL:Ce3+) were synthesized. The excitation and emission spectra and fluorescence decay curves of BLBL:Ce3+ were measured at room temperature. In BLBL:Ce3+ glasses, Ce3+ emits strong purplish-blue light. The results of analyzing spectra and fluorescence lifetime show that there are two kinds of Ce3+ luminescence centres in this kind of rare earth borate glasses. Ce3+ doped rare-earth glasses is a kind of beneficial supplement for Ce3+ fluorescence glasses system.

English Abstract↗
PubMed1998

Identification of two Escherichia coli pseudouridine synthases that show multisite specificity for 23S RNA.

Several putative Escherichia coli pseudouridine (Psi) synthases have been identified by iterative searching of genomic databases for ORFs homologous to known Psi synthases [Gustafsson et al. (1996) Nucleic Acids Res. 24, 3756-3762]. Of these, yceC and yfiI were proposed to encode Psi synthases which modify 23S rRNA. In the present work, yceC and yfiI were cloned and overexpressed in E. coli, and the encoded enzymes, YceC and YfiI, were purified to homogeneity. Both proteins converted Urd residues of rRNA to Psi, thus confirming their identities as Psi synthases. However, in in vitro experiments both enzymes extensively modified Urd residues of both 23S rRNA and 16S rRNA. Gene-disruption of yceCresulted in the absence of Psi modification at positions U955, 2504, and 2580 of 23S RNA, thus identifying these sites as in vivo targets for YceC. Likewise, yfiI disruption resulted in the absence of Psi modification at positions U1911, 1917, and possibly 1915 of 23S RNA. Disruption of yceC did not affect the growth under the conditions tested, whereas yfiI-disrupted cells showed a dramatic decrease in growth rate. Since YceC and YfiI hypermodify RNA in vitro, factors in addition to ribonucleotide sequence must contribute to the in vivo specificity of these enzymes.

Bacterial Proteins↗
PubMed1998

Analysis of thermal stability of soya globulins using monoclonal antibodies.

The epitopes of two monoclonal antibodies (Mabs), one raised to soya glycinin (IFRN 0025) and one to beta-conglycinin (IFRN 0089), have been defined. The epitope of 0025 corresponds to residues 86-104 of the acidic polypeptide of glycinin A1aB1b and lies at the C terminus of the proteolytic intermediate known as glycinin-T, whilst that of 0089 corresponds to residues 78-84 in the acidic extension present in the alpha' subunit of beta-conglycinin. As the Mabs bind to synthetic peptides corresponding to the epitope regions both epitopes can be considered as being continuous in nature. The regions recognised correspond to surface loops, which are probably flexible in nature. Both Mabs were used to investigate thermally induced conformational changes in soya globulins. Thermally treated glycinin was recognised more strongly than native protein, possibly due to disruption of the disulphide bond joining the acidic and basic polypeptides. Disruption could increase epitope accessibility, as could the conformational changes associated with denaturation. The binding of anti-beta-conglycinin Mab 0089 was unaffected by heating, suggesting that its epitope remains on the surface of the aggregates formed on heating. This study demonstrates that Mabs with defined specificities can be sensitive probes for monitoring local conformational changes within a protein molecule during thermal denaturation.

Amino Acid Sequence↗