Creating solutions to an ethical dilemma.
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Curriculum↗
Biomedical subjects
L Hernandez
Publications and source records attributed to L Hernandez.
Use of atomic force microscopy to reveal sperm ultrastructure in HIV-patients on highly active antiretroviral therapy.
Atomic force microscopy (AFM) has been employed to examine morphological and topographical changes caused by human immunodeficiency virus (HIV) and the effects of highly active antiretroviral therapy (HAART) on spermatozoon of HIV infected patients. This powerful technique has allowed us to visualize morphological alterations present in the spermatozoa of patients either with or without treatment. In addition to this, even the minute details, such as viral particles, located on the membrane of the spermatozoa, and the merging of such particles on the surface of the spermatozoa were detected with precision. The most important aspect is that AFM, unlike electron microscopy, permits to image virions in their nearly natural environment. Excess of damage of spermatozoon is due to the chemicals involved in HAART rather than the damage made by virus.
Antiretroviral Therapy, Highly Active↗
Analysis of brain neuropeptides by capillary electrophoresis: determination of luteinizing hormone-releasing hormone from ovine hypothalamus.
The use of capillary electrophoresis to analyze in vivo release of luteinizing hormone-releasing hormone from the ewe median eminence and tissue content of luteinizing hormone-releasing hormone from discrete brain areas (within and immediately adjacent to the median eminence) is described. Results were compared with those obtained with a specific luteinizing hormone-releasing hormone radioimmunoassay. In vivo release samples were obtained by a push-pull cannula procedure. The exact position of the median eminence in each animal was determined from lateral roentgenographs after infusion of a radiopaque dye into the third ventricle. Each ewe median eminence was perfused with an artificial cerebrospinal fluid solution and sampled at 20-min intervals. Brain tissues for luteinizing hormone-releasing hormone content were obtained after each animal was killed. Samples were either analyzed by capillary electrophoresis and UV detection at 210 nm or assayed with a specific luteinizing hormone-releasing hormone radioimmunoassay. Tissue homogenate content and release samples had several peaks resolved by capillary electrophoresis and UV detection. Luteinizing hormone-releasing hormone was identified as one of these peaks. Luteinizing hormone-releasing hormone was also shown to be present in these samples when evaluated by radioimmunoassay. A good correlation between capillary electrophoresis and radioimmunoassay was observed for luteinizing hormone-releasing hormone content values obtained from discrete hypothalamic areas.(ABSTRACT TRUNCATED AT 250 WORDS)
Animals↗