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Biomedical subjects

L Henry

Publications and source records attributed to L Henry.

At least 73 records · Page 4Linked to original sources
PubMed1998

Filling the gap: weaning practices of children aged 0-2 years in western metropolitan Melbourne.

OBJECTIVE: To investigate weaning practices of children aged 0-2 years in Melbourne's western metropolitan region (WMR). METHODOLOGY: The study group comprised children aged 0-2 years attending six maternal and child health centres in the City of Brimbank in Melbourne's WMR. One hundred and nineteen parents were asked to complete a questionnaire about their child. Interpreters were used as required. In total, 115 questionnaires were completed representing a response rate of 97%. All maternal and child health nurses working in the target region were asked to complete a questionnaire. Of these, five were selected randomly to participate in follow-up indepth interviews. Weaning was defined as the addition of food/fluids other than breast milk or formula. RESULTS: The mean age for the introduction of solids was 4.3 months. Of the children already introduced to solids, 67% had commenced between 4 and 6 months whilst 29% of the infants were taking solids by 3 months of age. Of the children already having juice, 85% had done so by the age of 6 months. The mean age for the introduction of cow's milk as the main drink was 10.7 months (S.D. 2.8). For English speaking families the mean age was 11.3 months (S.D. 1.8) whilst for families speaking languages other than English the mean age was 9.7 months (S.D. 4.1). CONCLUSIONS: Most parents in this group were following the recommended guidelines for weaning. Some parents had poor knowledge of appropriate weaning time, use of fruit juice and introduction of cow's milk as the main drink. Additional information and education is required in these areas. Further investigation is required in relation to use of cow's milk and ethnicity.

Australia↗
PubMed1998

Motion perception in global versus local attentional modes.

PURPOSE: Global and local attention are two forms of selective visual attention which activate different areas of the cortex. The purpose of this experiment was to test subjects' motion coherence thresholds under conditions of global or local attention. It was hypothesized that thresholds in global attention would be lower than in local attention. METHODS: Eleven adult subjects participated in this study. Subjects were required to identify direction of motion at variable coherence levels, while simultaneously identifying either the global or local letter. Three velocities were used for coherent motion (3, 6 and 18 degrees/s). RESULTS: The results showed that letter identification (global or local) did not significantly affect motion coherence thresholds; however, thresholds were significantly higher at 18 degrees/s than in the lower velocities. CONCLUSIONS: These results highlight the attentional limitations of visual information shown by increased motion coherence thresholds when two objects must be identified simultaneously in a brief display.

Adolescent↗
PubMed1998

Attentional blink in global versus local attentional modes.

PURPOSE: Brain imaging studies have shown that global and local attention activate different areas of the brain, with implication for dependence of perception on attentional state. The aim of the present experiment was to investigate the duration of the attentional blink in global versus local attention. METHODS: Rapid Serial Visual Presentation (RSVP) sequences of global/local stimuli were presented to 34 adult subjects who had to identify a target red figure followed by a determination of whether a certain probe letter, 'X' (specified to be either the global or local form), was present in the subsequent string of letters. RESULTS: Attentional blinks were longer than any previously reported (1.67 s global; 2.97 s local) and were significantly different.Thus, the length of the attentional blink is dependent on the attentional state of the subject. CONCLUSION: This pattern of results is consistent with the hypothesis that global attention mechanisms receive predominantly M-pathway input.

Adolescent↗
PubMed1998

Cognitively-oriented psychotherapy for early psychosis (COPE). Preliminary results.

BACKGROUND: The present study describes the results of the pilot testing of a therapy we have developed for people with first-episode psychosis. Cognitively-oriented psychotherapy for early psychosis (COPE) is aimed at facilitating the adjustment of the person, and at preventing or alleviating secondary morbidity in the wake of the first psychotic episode. METHOD: Eighty people formed three groups: those who were offered and accepted COPE (COPE subjects); those who refused COPE (refusal subjects); and those who were offered neither COPE nor any other continuing treatment from our service (control subjects). The individuals were assessed prior to, and at the end of, COPE treatment (a 12-month period) on the Integration/Sealing Over, Explanatory Model, Scale for the Assessment of Negative Symptoms, Brief Psychiatric Rating Scale, Quality of Life, SCL-90-R, and Beck Depression Inventory measures. RESULTS: People who received COPE obtained significantly superior scores (P < 0.05) to the control group on four of the seven measures but only significantly out-performed the refusal group on one of the seven measures (P < 0.05). The COPE group performed significantly worse on the BDI than the refusal group (P < 0.05). Effect sizes are also provided for each measure. CONCLUSIONS: There seems to be a place for psychological therapy in this group of people but our results need to be replicated in a more definitive randomised controlled trial and such a study is now in progress.

Adult↗
PubMed1997

Changes in the subunit distribution of prosomes (MCP-proteasomes) during the differentiation of human leukemic cells.

The subunit composition of cell-internal and surface prosomes during phorbol myristate acetate (PMA)-induced differentiation of human leukemic T lymphocytes (CCRF-CEM cell line) was studied in relation to clusters of differentiation (CD) markers. PMA inhibited cell growth and decreased the amounts of CD1a and CD4 while CD3, CD8, CD25, CD45, CD57 and MHCI increased it; the p53 anti-oncogene increased while actin levels remained constant. Cells incubated with the inducer PMA for 3 days and placed in fresh inhibitor-free medium resumed growth at a low rate, while the CD values slowly reverted to those of the initial phenotype. The presence and relative amounts of prosome subunits were analyzed by flow cytometry, light and fluorescent microscopy and Western blotting using 3 monoclonal antibodies (p25K, p27K and p30-33K MAbs). The decrease in cytoplasmic antigens on day 3 was remarkable (cells followed for 7 days) while increased surface antigens were observed. Changes in the subcellular distributions of prosome antigens, particularly the p25K and p30-33K subunit, were correlated with a partial arrest of the cell cycle. Interestingly, the composition of cell internal and surface prosomes showed different patterns of change.

Antibodies, Monoclonal↗
PubMed1997

Structural adaptations in the specialized bacteriophage T4 co-chaperonin Gp31 expand the size of the Anfinsen cage.

The Gp31 protein from bacteriophage T4 functionally substitutes for the bacterial co-chaperonin GroES in assisted protein folding reactions both in vitro and in vivo. But Gp31 is required for the folding and/or assembly of the T4 major capsid protein Gp23, and this requirement cannot be satisfied by GroES. The 2.3 A crystal structure of Gp31 shows that its tertiary and quaternary structures are similar to those of GroES despite the existence of only 14% sequence identity between the two proteins. However, Gp31 shows a series of structural adaptations which will increase the size and the hydrophilicity of the "Anfinsen cage," the enclosed cavity within the GroEL/GroES complex that is the location of the chaperonin-assisted protein folding reaction.

Amino Acid Sequence↗
PubMed1997

The pathological and biological nature of screen-detected breast carcinomas: a morphological and immunohistochemical study.

Traditional and immunohistochemical markers of prognosis were examined in 455 mammary carcinomas derived from breast cancer screening and compared with those of 277 carcinomas presenting symptomatically over the same period. Tumours detected by population screening under the U.K. National Health Service Programme do not differ from those detected by other screening projects, but compared with symptomatic cancers, screen-detected cancers are more likely to be in situ and if invasive, to be smaller, of lower grade, and to have invaded vessels, perineural spaces, and lymph nodes less frequently. Tubular and cribriform types are more often represented in screened patients. Immunohistochemical markers which have been proposed as being related to likely tumour behaviour (epidermal growth factor receptor, c-erbB-2 protein, oestrogen and progesterone receptors, cathepsin D, p53, and retinoblastoma protein) do not distinguish screen-detected from 'clinical' cancers. It is concluded that cancers diagnosed at screening do not differ biologically from those presenting clinically, but are the same lesions detected at an earlier stage of their natural history.

Biomarkers, Tumor↗
PubMed1997

New monoclonal antibodies to oestrogen and progesterone receptors effective for paraffin section immunohistochemistry.

Assessment of oestrogen and progesterone receptors (ER and PgR) in breast cancer is widely used for the prediction of response to endocrine therapy and as a prognostic marker. Cytosolic assays have been replaced in many centres by immunochemical techniques, which have many advantages including applicability to small samples, simplicity, and cost-effectiveness. This study describes the generation and characterisation of two novel murine monoclonal antibodies recognizing ER and PgR, designated NCL-ER-6F11 and NCL-PGR respectively, which are effective in heat-treated formalin-fixed, paraffin-embedded tissue. The antibodies have been characterized by Western blotting and by immunohistochemistry on normal and pathological breast and other tissues. NCL-ER-6F11 has been shown to compare favourably with a currently available ER antibody. These antibodies may prove of value in the assessment of hormone receptor status in human breast cancer.

Antibodies, Monoclonal↗
PubMed1997

Prosomes (proteasomes) changes during differentiation are related to the type of inducer.

The core of the 26S proteasome, the 20S prosome, is a highly organized multi-protein complex found in large amount in malignant cells. Differentiation of several cell lines, including the monoblastic U937 and the lymphoblastoid CCRF-CEM, is accompanied by a general decrease in the prosome concentration when phorbol-myrirtic-acetate (PMA) and retinoic acid plus dihydroxyvitamine D3 (RA+VD) are used. Incubation of U937 cells for three days with PMA or RA+VD causes differentiation, but the resulting patterns of prosome labeling in the cell and on the plasma membrane are not the same. In contrast, the same kind of prosome changes occur in U937 and CCRF-CEM cells when PMA is used as inducer. The intracellular distribution of prosomes is also linked to malignancy and differentiation. Prosomes are found in the nucleus and the cytoplasm of cancer cells; and treatment with RA+VD decreases the prosomes in the nucleus whereas PMA causes various prosome proteins changes. These results indicate that prosomes are important in cell regulation and in the expression of malignancy.

Cell Differentiation↗
PubMed1997

Transfer across form and modality in implicit and explicit memory.

Three experiments examined transfer across form (words/pictures) and modality (visual/auditory) in written word, auditory word, and pictorial implicit memory tests, as well as on a free recall task. Experiment I showed no significant transfer across form on any of the three implicit memory tests, and an asymmetric pattern of transfer across modality. In contrast, the free recall results revealed a very different picture. Experiment 2 further investigated the asymmetric modality effects obtained for the implicit memory measures by employing articulatory suppression and picture naming to control the generation of phonological codes. Finally, Experiment 3 examined the effects of overt word naming and covert picture labelling on transfer between study and test form. The results of the experiments are discussed in relation to Tulving and Schacter's (1990) Perceptual Representation Systems framework and Roediger's (1990) Transfer Appropriate Processing theory.

Auditory Perception↗
PubMed1997

Proteasome (prosome) subunit variations during the differentiation of myeloid U937 cells.

20S proteasomes (prosomes/multicatalytic proteinase) are protein particles built of 28 subunits in variable composition. We studied the changes in proteasome subunit composition during the differentiation of U937 cells induced by phorbol-myristate-acetate or retinoic acid plus 1,25-dihydroxy-cholecalciferol by western blot, flow cytometry and immuno-fluoresence. p25K (C3), p27K (IOTA) and p30/33K (C2) subunits were detected in both the nucleus and cytoplasm of undifferentiated cells. Flow cytometry demonstrated a biphasic decrease in proteasome subunits detection during differentiation induced by RA + VD. PMA caused an early transient decrease in these subunits followed by a return to their control level, except for p30/33K, which remained low. Immuno-fluorescence also showed differences in the cytolocalization of the subunits, with a particular decrease in antigen labeling in the nucleus of RA + VD-induced cells, and a scattering in the cytoplasm and a reorganization in the nucleus of PMA-induced cells. Small amounts of proteasomal proteins were seen on the outer membrane of non-induced cells; these membrane proteins disappeared when treated with RA + VD, whereas some increased on PMA-induced cells. The differential changes in the distribution and type of proteasomes in RA + VD and PMA-induced cells indicate that, possibly, 20S proteasomes may play a role in relation to the mechanisms of differentiation and the inducer used.

Animals↗
PubMed1997

Immunocompromised patients and nutrition.

Most immunocompromised patients will have undergone bone marrow transplantation. These patients will require both nutritional support and some form of dietary restriction. Food restrictions used with patients undergoing BMT include a sterile diet, low-microbial diet or modified hospital diet. No empirical research exists on the relative benefits of each.

Bone Marrow Transplantation↗
PubMed1997

[Magnetic resonance study of complex anal fistulae. Preliminary results apropos of 28 cases].

PURPOSE: To evaluate the use of Magnetic Resonance Imaging (MRI) for the treatment of problematical anal fistulas and follow-up of cases treated by long-term seton drainage with two or three-stage fistulotomy. METHOD: prospective study on 24 patients with surgical treatment between July 1993 and March 1995 selected for MRI among 100 anal suppurations according to following criteria: high trans-sphincteric or supra-sphincteric fistula with one or more previous definitive attempts at treatment (15), and/or severe anal or rectal fibrosis (11). Patients with Crohn's disease were not included. For 14 of these patients who had a long-term seton drainage, MRI tried to assess the quality of the surgical drainage and the course of residual abscess. RESULTS: the overall accuracy of MRI for visualization of internal opening, primary and secondary tract was 84% when compared with surgical data. The sensitivity and specificity were 100% for the detection of horse shoe fistulous tracts (8). For the 14 patients followed by MRI, only one false positive was noted and in 5 cases the results of MRI modified the previous staged procedure (prolonged seton drainage or new excision). For the 20 patients treated for more than 6 months, the mean follow-up was 18 months and median was 14 months. Only one recurrence was noted with no loss of follow-up. CONCLUSION: this study confirms the high degree of accuracy of MRI for mapping of high complex fistula in ano. MRI is very helpful for the survey of healing in cases treated with seton drainage and two or three stage fistulotomy to preserve continence.

Adult↗
PubMed1997

Parenteral nutrition.

Patients with a non-functioning gastrointestinal tract, those who require bowel rest or cannot receive enteral nutrition will be considered for parenteral feeding. This Update looks at indications, venous access, administration and complications.

Gastrointestinal Diseases↗
PubMed1996

Methods, indications, and results of percutaneous choledochoscopy. A series of 161 procedures.

OBJECTIVE: Nonsurgical methods for evaluation and treatment of the biliary tree are usually done under fluoroscopic guidance. Direct visualization of the bile ducts, that is, choledochoscopy, could provide a more precise method with which to perform these maneuvers. The methods, indications, and results of percutaneous choledochoscopy are discussed from a series of 161 procedures performed in 123 consecutive patients. METHODS AND RESULTS: Ninety-six patients had transhepatic drains and 27 had T-tubes implanted. Biopsy of bile duct stenosis was conducted in 52 cases. The sensitivity for the diagnosis of malignancy was 78%, and the authors recommended this method in case of nonsurgical treatment of stenosis. Gallstone extraction was performed in 75 cases (35 intrahepatic). Lithotripsy was necessary for 64 patients and complete gallstone clearance was obtained for 69 patients (92%). The rate of stone recurrence was 18.1% (median follow-up, 32 months), prompting the authors to reconsider surgery for the treatment of underlying disease. Laser photocoagulation was used successfully for two of three patients to ablate an intraductal adenoma. In eight cases of cholangiocarcinoma, the laser effect was too short to satisfactorily relieve obstruction of the intrahepatic bile ducts. Morbidity dramatically decreased from 53.7% to 5% when progressive dilation of the tract, aseptic conditions, and general anesthesia were applied systematically. CONCLUSION: Percutaneous choledochoscopy can be used routinely, provided that strict techniques are adhered to. This procedure should be reserved for only a small subset of biliary diseases.

Adolescent↗
PubMed1996

Changes in the amount and distribution of prosomal subunits during the differentiation of U937 myeloid cells: high expression of p23K.

Prosomes (Proteasomes/Multicatalytic proteinase (MCP)-complexes) are protein particles built of 28 subunits in variable composition, having proteinase activity. We have studied the changes in prosomal subunits p29K, p31K and the highly expressed p23K during the differentiation of U937 cells. Control cells had little prosomal subunit p31K in the cytoplasm, while p29K antigen was detected in both the nucleus and cytoplasm; more p23K antigen was found in the cytoplasm than in the nucleus. Flow cytometry demonstrated a biphasic intracellular decrease in prosomes during differentiation induced by phorbol-myristic-acetate (PMA) and retinoic acid plus 1,25-dihydroxycholecalciferol (RA + VD). p23K and p29K decreased both in the cytoplasm and the nucleus of differentiated cells, though the p23K antigen was concentrated near vesicles and the plasma membrane in PMA-induced cells. The p31K antigens disappeared from RA + VD-induced cells, while in PMA-induced cells, cytoplasmic labelling was unchanged and nuclear labelling was increased. Small amounts of prosomal proteins p23K and p29K were found on the outer membrane of un-induced cells. While there was no labelling on the outer membrane of RA + VD-induced cells, p23K protein increased on the plasma membrane of PMA-induced cells. The prosome-like particle protein p21K was not present to any significant extent in the intracellular compartment of control or induced cells; however, p21K was detected on the outer surface of control cells and was increased only in PMA-induced cells. The culture medium of control and induced cells contained no p21K, p23K, p29K or p31K. RA + VD seemed to induce a general decrease of prosomal subunits within the cells and at the outer surface, whereas PMA caused a migration toward the plasma membrane and an increase at the outer surface. These changes in the distribution and type of prosomes in RA + VD- and PMA-induced cells indicate that prosomes may play a part in differentiation, especially p23K which is the most highly expressed protein among those studied and presents the more important changes.

Carcinogens↗
PubMed1996

Working memory in children with autism and with moderate learning difficulties.

We asked whether children with autism are specifically impaired on tests of working memory. Experiment 1 showed that children with autism were at least as likely as normal children to employ articulatory rehearsal (criterion: evincing the "word length effect") and that they had superior spans to that of children with moderate learning difficulties. In Experiment 2, participants were given "capacity tasks" in order to examine group differences in the capacity of the central executive of working memory. The performance of the children with autism was inferior to that of the normally developing group and similar to that of the children with moderate learning difficulties.

Autistic Disorder↗