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Biomedical subjects

L He

Publications and source records attributed to L He.

At least 361 records · Page 20Linked to original sources
PubMed1995

[Production and application of mouse antiserum to human estrogen receptors].

An antiserum to peptide containing 15 amino acids corresponding to the region-D of human estrogen receptors (hERD) was obtained in mice by immunization with the peptide conjngated to KLH. Using this antiserum, the ER status of paraffin-embeded sections of 95 human breast carcinomas (in which, sections of 31 were both frozen and paraffin-embeded ones) were studied. The corresponding rate for determination of ER status between immunohistochemical staining (IHC) and dextran coated charcoal (DCC) assay was 89.5%. The concordance rate for semiquantitative gradings was 69.3%. In addition, in situ hybridization (ISH) of 15 frozen sections of the samples using digoxigenin labeled dUTP to identify the expression of ER mRNA was also done. The result of ISH was fully consistent with that of IHC (100%). The results show that the mouse antiserum to hERD obtained in this study is specific and sensitive for IHC assay of ER and IHC is a valuable adjunct and/or alternative to the biochemical method for determination of the ER status of breast cancer.

Animals↗
PubMed1995

[The effect of different topical agents on pseudomonas infection of burn wound].

48 Wistar rats were scalded resulting in 15% TBSA full-thickness burn. Pseudomonas aeruginosa (10(9)/ml was inoculated to the wounds. The rats were divided into 6 groups. 5 different topical agents were separately applied to the wounds constituting 5 groups, and no drug was used in control group. Through observing the appearance of wound, the quantity of bacteria in the subeschar tissue and histo-pathological changes, we confirmed that AgSD-ZnSD-A cream was a satisfactory topical agent.

Animals↗
PubMed1995

[Study of DNA content, immunophenotype in childhood non-Hodgkin's lymphomas].

The DNA content, immunophenotypes and Ag-NOR of 63 cases of childhood NHL have been studied with flow cytometry and immunohistochemistry. The results showed that the incidence of aneuploidy, the numbers of Ag-NOR and PI increased significantly with increasing histological grade. The numbers of PI were also a valuable prognostic parameter. There was a positive correlation between the numbers of PI and AgNOR. No significant correlation was seen between the survival rates of diploid and aneuploid tumors. The highest to lowest survival rates being in the sequence of unclassified tumors, T-cell tumors and B-cell tumors.

Adolescent↗
PubMed1995

[Vitrectomy combined with scleral buckling for the treatment of complicated retinal detachment].

PURPOSE: To raise operative successful rate of complicated retinal detachment, we used the methods of vitrectomy combined with scleral buckling for the treatment of complicated retinal detachment. METHODS: The operative methods included: scleral buckling, vitrectomy, membrane peeling, intraocular and extraocular drainage, gas and silicone oil tamponade. RESULT: Being followed up for 1-16 months, 22 eyes got anatomic reattachment in 29 eyes, the operative successful rate was 75.8%; 15 eyes were injected with silicone oil intraocular tamponade, with 12 eyes getting reattachment. The successful rate was 80%. CONCLUSION: Since the vitreous surgery is used, it is possible for the treatment of complicated retinal detachment, the operative successful rate can be raised. The technique of membrane peeling and intraocular tamponade was very important. Silicone oil has been accepted by more and more ophthalologists in the world, which acts as an effective material of intraocular tamponade.

Adolescent↗
PubMed1994

Expression of vesicle-associated membrane protein 2 (VAMP-2)/synaptobrevin II and cellubrevin in rat skeletal muscle and in a muscle cell line.

Molecular studies have identified a family of synaptic vesicle-associated membrane proteins (VAMPs, also known as synaptobrevins) which have been implicated in synaptic vesicle docking and/or fusion with plasma membrane proteins. Here we demonstrate the expression of two members of this family, VAMP-2/synaptobrevin II and cellubrevin, in skeletal muscle, a tissue with both constitutive and regulated membrane traffic. The 18 kDa VAMP-2 polypeptide was detected in purified membrane fractions from adult skeletal muscle and from L6 myotubes in culture, demonstrating that the presence of this protein in the isolated muscle membrane fractions is not the result of contamination by ancillary tissues such as peripheral nerve. Furthermore, skeletal muscle and the muscle cell line also expressed cellubrevin, a VAMP-2 homologue of 17 kDa; which is much less abundant in brain cells. Both VAMP-2 and cellubrevin were preferentially isolated in membrane fractions rich in plasma membranes, and were less concentrated in light microsomes and other internal membrane fractions of mature muscle or muscle cells in culture. Interestingly, both VAMP-2 and cellubrevin were much more abundant in the differentiated L6 myotubes than in their precursor myoblasts, suggesting that they are required for functions of differentiated muscle cells. The identity of both polypeptides was further confirmed by their susceptibility to proteolysis by Clostridium tetanus toxin. Expression of these products was further established by the presence of mRNA transcripts of VAMP-2 and cellubrevin, but not of VAMP-1, in both skeletal muscle and L6 myotubes. In contrast, other synaptic vesicle and docking/fusion components were undetectable, such as VAMP-1, SNAP25 and syntaxin 1A/1B, as were synaptophysin and synapsin Ia/Ib, proteins which are believed to be involved in sensing the signal for neuronal exocytosis. It is concluded that VAMP-2 and cellubrevin are expressed in skeletal muscle cells and may each participate in specific processes of intracellular membrane traffic.

Amino Acid Sequence↗
PubMed1994

Body mass, fat distribution and cardiovascular risk factors in a lean population of south China.

The associations of body mass index and abdominal adiposity, represented by an elevated waist/hip circumference ratio, with cardiovascular risk factors were examined in men and women, aged 28-69 years, from urban and rural areas of Guangzhou, China. Mean body mass index ranged from 20.1 to 21.9 kg/m2 across the four sex- and area-groups. Mean waist/hip ratio was 0.84 in men and 0.80 in women. After accounting for age and body mass index, waist/hip ratio was associated negatively (p < 0.05) with fasting serum HDL cholesterol (both sexes), and positively with serum triglycerides (both sexes), total and LDL cholesterol (men only), uric acid (both sexes), glucose (women only), and mean systolic blood pressure (women only). Body mass index was associated in a similar direction with most of these risk factors. These data confirm that abdominal adiposity is independently associated with cardiovascular disease risk factors, even in a lean Asian population.

Abdomen↗
PubMed1994

[Therapeutic effectiveness of zuzhongping on patients with arteriosclerostic cerebral infarction].

Forty-six patients with acute arteriosclerostic cerebral infarction were randomly divided into two groups: control group and treatment group. Each of them included 23 patients respectively. The patients in the control group were given Dextran-40 but the ones in the treatment group were given the mixture of Zuzhongping. The course of treatment was 3 weeks. It was found that there was a significant difference (P < 0.01) in the score percentage, before and after treatment of neurological defects, between the control group and the treatment group, and the former (29.70 +/- 33.52) was much lower than the latter (45.40 +/- 27.60). The total curative rate of the treatment group (87.0%, 20/23) was significantly higher than that of the control group (60.9%, 14/23). There was an obviously prolonged KPTT (kaolin partial thromboplastin time) value and a decreased Fb (fibrinogen) level in the treatment group. Before treatment they were 32.43 +/- 4.03 sec and 6.18 +/- 1.77 g/L respectively, but after treatment, 52.96 +/- 10.50 sec and 4.5 +/- 0.95 g/L respectively. The authors suggest that the significant therapeutic efficacy of Zuzhongping in the patients with acute arteriosclerostic cerebral infarction is related to its action of anticoagulation, modification of PGI2 and TXA2 level in the body, decreased blood Fb level, hyperglycemia, etc.

Aged↗
PubMed1994

[Relationship between summating potentials and perilymphatic fistula].

Serial electrocochleogram (ECochG) recordings were obtained within 24 hours from 40 guinea pigs with experimentally induced PLF, and the cochlears of 30 guinea pigs (60 ears) were histopathologically observed under light microscope. -SP occurred in 100% experimented ears within 1-3 hours after PLF, but distention of Ressner's membrane was found only in 30% ones. CAP thresholds and N1 latencies of 10 animals were improved, accompanying disappearance of -SP in 6 of the 10 animals in 24 hours after PLF. It is presumed that -SP is not a specifically electrophysiologic indicator of ELH, and that the generation of -SP may chiefly result from reversible damage of cochlear hair cells. The mechanical factor of basilar membrane displacement can hardly be considered as a main cause of SP abnormality. The mechanism of the ELH following PLF was also discussed.

Animals↗
PubMed1994

[Histopathology of nonacoustic labyrinth following head injury in guinea pigs].

The histopathological changes in nonacoustic labyrinth induced by experimental head injury were studied in 11 guinea pigs. The animals were divided into two groups. The first group were killed after the pain sense recovered and the second group were allowed to survive for 15 days. The temporal bones were serially sectioned and observed under light microscope. The pathological findings of the vestibular organs included arrangement disturbance, lytic, exfoliate and vacuolization of the sensory epithelia, massive spherical bodies in the region of cilium. The otolithic membranes were exfoliated in the utricular and saccular maculae. There was the otolith by the ductus reuniens separated from the saccular maculae in one ear. There were basophilic staining homogenous deposit on the cristae ampullaris. These findings showed that impairments of vestibulae following head injury were obvious. The secondary impairments, cupulolithiasis and obstruction of the ductus reuniens, from the utricular and saccular maculae were one of the pathologic changes in hearing loss and vertigo following head injury.

Animals↗
PubMed1994

[Changes in vestibular function and furosemide test after experimental endolymphatic hydrops in guinea pigs].

The present study reports a successful anial model of endolymphatic hydrops (EH) in guinea pigs (n = 48) through a surgical obliteration of the endolymphatic sac and duct. Electronystagmography was calibrated by optokinetic nystagmus. During the formation of EH the vestibular functions (n = 16) were dynamically observed by ratation test. After eight weeks (n = 25) the furosemide tests with different doses were carried out by sinusoidal angular acceleration test. The results showed that the excitability of vestibular function in the operated ears was enhanced for the first week after operation, and then declined. At the eighth week it was significantly improved after intravenous injection of furosemide at the clinical equivalent dose level (1.25 mg/kg). The present results strongly support the usefulness of furosemide test in the diagnosis of EH. The possible mechanisms of its effect are discussed.

Animals↗
PubMed1994

[The effects of electroacupuncture treatment on nucleolar organizer regions of adrenal cortex in ovariectomized rats].

The present paper reports the morphometric analysis of nucleolar organizer regions (NORs) of the adrenal cortex in ovariectomized rats following electroacupuncture (EA) using argyrephil (Ag-NOR) method for NORs. Animals were divided into four groups, the control group (CT group, n = 4), the EA group (n = 3), the ovariectomized group (OV group, n = 4) and the ovariectomized electroacupuncture group, (OV+EA group, n = 7). The number of AgNORs of 100 cells from zona fasciculata of the adrenal cortex in each case of all groups was counted at random and the mean +/- SE (number/cell) in each group was calculated as follows: OV+EA group 2.71 +/- 0.26, OV group 1.62 +/- 0.15, EA group 1.21 +/- 0.04 and CT group 1.48 +/- 0.03. The mean of AgNORs in OV+EA group differed highly significantly from the other three groups (P < 0.01) tested by ANOVA and LSD method, No significant distinction was found among the OV group, EA group and CT group. Gross specimen examination showed that adrenal glands in OV+EA were significantly heavier than those in the other three groups (P < 0.01). Vaginal smears showed that a response like that of estrogen-induced with exfoliative cells appeared in the OV+EA group rats following EA. EA had no influence on the change of exfoliative cells in EA group. The results suggest that EA may promote the synthesis and secretion of the adrenal steroid hormones, the androgen of which will then be transformed into estrogen in other tissues, thus compensating the deficiency of estrogen induced by ovariectomy.

Adrenal Cortex↗
PubMed1994

[Chemical constituents of flower of David lily].

Three compounds were isolated from the flower of Lilium devidii. On the basis of chemical reaction, UV, MS, 1HNMR and 13CNMR spectral data, they were identified as beta-sitosterol, stigmasterol and emodin. These compounds are obtained from the plant for the first time.

Drugs, Chinese Herbal↗