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Biomedical subjects

L He

Publications and source records attributed to L He.

At least 235 records · Page 13Linked to original sources
PubMed1999

[Hemodynamic monitoring in 52 serious burn patients in ten years].

OBJECTIVE: To determine the benefit of hemodynamic monitoring for resuscitation of burn shock. METHODS: A group of 52 burned patients with mean burn area of (69.9 +/- 20.1)% TBSA burn injury (range 31%-100%), and mean III burn area of (60.4 +/- 13.6)% TBSA were included in this study. Swan-Ganz catheters were inserted to monitor hemodynamics including RAP, PAP, PAWP, HR, CO, CI, and SI, etc. after admission at 8, 16, 24, 36, 48, 72, and 96 hours postburn. RESULTS: It was reasonably safe to perform invasive monitoring during early resuscitation. With the guide of hemodynamic monitoring, evidence of global hypovolemia disappeared at 24 hours after burn injury with appropriate resuscitation therapy. CONCLUSION: Invasive hemodynamic monitoring may be necessary to optimize resuscitation of serious burn patients with reasonable safety.

Adolescent↗
PubMed1999

[Enhanced antitumor effects induced by lymphotactin gene-modified dendritic cells after pulsed with tumor antigen peptide].

OBJECTIVE: To evaluate the potent effects of lymphotactin on tumor immunogene therapy and to improve the therapeutic efficacy of dendritic cells (DCs)-based vaccine, the protective and therapeutic effects of tumor antigen peptide-pulsed lymphotactin gene-modified dendritic cells were investigated. METHODS: In the tumor model of 3LL Lewis lung carcinoma, mouse bone marrow DCs transduced with mouse lymphotactin gene by adenovirus vector (Ltn-DC) were pulsed with MHC-I-restricted, 3LL cell-specific tumor peptide Mut 1 (FEQNTAQP), and used to vaccinate syngeneic mice or to treat the preestablished tumor-bearing mice with spontaneous pulmonary metastases. RESULTS: Immunization with Mut 1 peptide-pulsed Ltn-DC induced specific CTL against 3LL cells and induced protective antitumor immunity, which rendered the immunized mice resistant completely to 3LL tumor challenge. In vivo depletion of immune cell subsets with mAbs demonstrated that the protective immunity induced by Mut1 peptide-pulsed Ltn-DC in the induction phase was dependent on both CD4+ T cells and CD8+ T cells rather than NK cells, and in the effector phase on CD8+ T cells rather than CD4+ T cells or NK cells. CD28/CTLA4 pathway of T cell costimulation and IFN-gamma were also necessary for induction of antitumor immunity by Ltn-DC pulsed with Mut1 peptide. Treatment with Mut1 peptide-pulsed Ltn-DC significantly inhibited the 3LL spontaneous pulmonary metastases of the preestablished tumor-bearing mice and exhibited obvious therapeutic effects. CONCLUSIONS: Our data suggest that Ltn gene modified DCs are more potent in the induction of protective and therapeutic antitumor immunity through the preferential chemotaxis of DCs on T cells. Vaccination with tumor antigen-pulsed Ltn-DC may be a novel approach to immunotherapy of cancer.

Animals↗
PubMed1999

[Study on inflammatory response and alteration of biochemical components in BALF from Pneumocystis carinii pneumonia].

OBJECTIVE: To study the cellular and biochemical changes in airways in pneumocystis carinii pneumonia (PCP). METHODS: The cellular and biochemical changes in bronchoalveolar lavage fluid (BALF) between PCP and bacterial pneumonia in rat models induced by cortisone acetate(GC) injections were compared. The activities of alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and type IV collagenase(matrix metalloproteinases, MMP-2, MMP-9) in BALF were measured. The rats were divided into four groups: (1) normal controls: 6 healthy SD rats; (2) negative control: 6 rats with GC injection over 8 weeks without lung infection; (3) bacterial pneumonia: 11 rats with GC injection over 8 weeks complicating bacterial pneumonia without other pathogens isolated; (4) PCP: 14 rats with GC injection during 8-12 weeks complicating PCP without other pathogens isolated. RESULTS: In PCP the total cell counts (6.9 +/- 2.6) x 10(6)/L and the percentage of PMNs (18.6 +/- 6.0)% in BALF were increased compared with negative control(P < 0.01), but were lower than those in bacterial pneumonia group(P < 0.01). ALB in BALF in PCP (623 +/- 484) g/L was increased compared with negative control (36 +/- 16) g/L(P < 0.05) and bacterial pneumonia(91 +/- 43) g/L(P < 0.05). The activities of ALP and LDH in PCP [(217 +/- 81) U/L, (208 +/- 82) U/L respectively] were higher than those in negative control [(75 +/- 37) U/L, (86 +/- 61) U/L, P < 0.05)] and bacterial pneumonia [(90 +/- 45) U/L, (130 +/- 75) U/L, P < 0.05]. The activities of Type IV collagenase, including MMP-2, MMP-9, were increased in PCP compared with negative control (P < 0.001) and bacterial pneumonia (P < 0.001, P < 0.01), and the activities of MMP-2 and MMP-9 had positive correlations with total cell counts in BALF (r = 0.652, P < 0.05; r = 0.803, P < 0.001). CONCLUSIONS: It suggests that there are differences in the cellular and biochemical aspects of lung inflammation and injury between PCP and bacterial pneumonia.

Animals↗
PubMed1999

[Gingival crevicular fluid enzyme levels at different destruction sites in the same teeth].

OBJECTIVE: To direct selecting proper sampling methods and sites in clinical research. METHODS: Gingival crevicular fluid (GCF) was sampled separately at the mesial-buccal and distal-buccal sites in the same teeth, which were destroyed differently. Clinical parameters were recorded in the subject sites and its adjacent sites. Aspartate Aminotransferase (AST) and Alkaline Phosphatase (ALP) in the GCF were determined. Difference of the enzyme levels between the two sampling sites, and relations between the enzyme level and all the clinical parameters were assessed. RESULTS: GCF-AST, ALP per sample at severely diseased sites were much higher than that from healthy and/or mild sites in the same teeth. Significant positive correlation (P < 0.001, r > 0.5) was demonstrated between enzyme levels in the sampling sites and probing depth (PD) and attachment level (AL) of the lingual sites. However, there was mild significant correlation (P < 0.05, r = 0.275) between ALP level in the sampling sites and PD of the proximal-buccal sites of its adjacent teeth. No significant correlation (P > 0.05) was found between AST level in the sampling sites and PD, AL of the adjacent teeth sites, and also between ALP level in the sampling sites and AL of the adjacent teeth sites. CONCLUSION: Site-specificity of periodontal disease was further demonstrated. GCF components were influenced reciprocally more obviously in the same proximal than by other sites.

Adult↗
PubMed1999

[Gastric colonization and its effect on pathogenesis of nosocomial pneumonia].

OBJECTIVE: To study the gastric colonization and its effect on development of nosocomial pneumonia (NP). METHODS: We investigated the microorganism (mainly pathogens) constitution, isolating sequences and risk factors in gastric, oropharyngeal and lower respiratory tract flora. Microbiologic cultures of serially taken samples of gastric aspirates, oropharyngeal swabs and bronchoalveolar lavages (BALs) or sputums were performed at the time of ICU admission and subsequently every morning in 52 surgical patients with nasogastric tube. Etiologic diagnosis of NP was based on quantitative cultured of BAL or sputum. Sequences of colonization were examined by comparing isolates of the same species with concordance of minimum inhibitory concentration values to 12 antibiotics. RESULTS: A total of 237 pairs of gastric and oropharyngeal specimens were taken. 77 strains of gram- negative bacilli (GNB) isolated from stomach, more than the number of GNB isolated from oropharynx (51 strains), P = 0.007. Comparing the species, Enterobacteriaceae isolated from stomach was much more than that from oropharynx (46 vs 24 strains, P = 0.004), and in most instances stomach colonization was before oropharynx colonization one to two days. Isolating rates of Pseudomonas were the same in both kinds of specimens. On contrast, Acinetobacter colonization in oropharynx was much more than that in stomach (10 vs 3 strains, P = 0.049). Risk factors for GNB colonization in stomach included increased gastric pH, medications inhibiting gastric acid secretion (such as cimitidine, omeprazole), and systemic use of cephalosporins. 10 patients developed NP and 5GNB (Enterobacter cloacae 2, Pseudomonas aeruginosa 2, Acinetobacter anitratum 1) isolated from BAL or sputum in 4 of 10 patients. A sequence of colonization from the stomach to the respiratory tract leading to pneumonia was observed in 3 patients. CONCLUSIONS: It was suggested that the stomach is likely to be an important source of pathogens leading to NP, especially Enterobacteriaceae pneumonia.

Adult↗
PubMed1999

[Precise positioning for reconstruction of anterior cruciate ligament].

OBJECTIVE: To supply the basement for precise positioning for anterior cruciate ligament (ACL) reconstruction in the knee and recommend a precise measurement method during the operation. METHODS: Bone tunnels along ACL attachments were made in 20 cadaveric knees. Steel wire was introduced through the tunnel and the length changes were measured during passive knee flexion. We developed an Isometer for getting continuous parameters. RESULTS: Anterosuperior (AS) fibers were lengthened during knee flexion from 30 to 120 degrees, combined with the increase of anterior restraints. Length changes of the posterosuperior and central (C, PS) fibers were minimal. The length of the anteroinferior and posteroinferior (AI and PI) fibers were shortened with the decrease of the anterior restraints. CONCLUSIONS: The central and posterosuperior (C, PS) parts of femoral attachment would be the ideal reconstruction point. In case of old injury of ACL with ambiquous landmark, the Isometer is suggested in identifying the reconstruction point.

Anterior Cruciate Ligament↗
PubMed1999

[Effect of escharectomy during burn shock stage on stress response].

OBJECTIVE: To investigate changes in hormones of plasma and the effect of escharectomy during burn shock stage on early stress response after burn. METHODS: Sixteen male minipigs were subjected to a 35% TBSA III degree burn injury. The burned minipigs were randomly divided into two groups: escharectomy during burn shock stage group (S group) and escharectomy group after burn shock stage (N group). The sequential changes in plasma levels of insulin, glucagon, cortisol, GH, IGF-I, albumin were analyzed. RESULTS: The serum concentration of insulin, glucagon, cortisol increased to some degree, whereas the serum concentration of GH, IGF-I decreased obviously after burn. As compared with the N group, no significant difference in the serum concentration of glucagon, cortisol, GH, IGF-I, was observed. CONCLUSIONS: Escharectomy during burn shock stage could not aggravate stress response after burn.

Animals↗
PubMed1999

[The effect of early escheractomy on expression of ICAM-1 and E-selectin mRNA of endothelial cells].

OBJECTIVES: To examine the effect of burned serum on change in endothelial cell ICAM-1 and E-selectin mRNA expression and whether these alterations can be corrected by the means of escharectomy during burn shock period. METHODS: One hundred and seventy-six Wistar rats with 30% III degree TBSA on back were studied. Endothelial cell ICAM-1 and E-selectin mRNA were tested with the method of RT-PCR. RESULTS: ICAM-1 and E-selectin expression showed an increase after stimulation by serum of 4 hours post burn, reaching peak levels 168 hours after injury. ICAM-1 and E-selectin expression levels could decrease to baseline within 168 hours in the rats which underwent escharectomy during shock stage, but kept higher than normal in rats without escharectomy or underwent 96 hours post burn. CONCLUSIONS: These findings suggest that eschar can induce endothelial cells to express adhesion molecule. Early escharectomy is important to prevent adhesion molecule expression and SIRS.

Animals↗
PubMed1999

[Quality of life as a time-dependent covariable in cox regression analysis and its application].

OBJECTIVE: To present a statistical method for combined evaluation of the quality of life and survival time and to study some issues in its application. METHODS: Based on Cox regression model, quality of life was used as a time-dependent covariable in analysis of the influence factors for the effectiveness of detoxification in 212 drug addicts. RESULTS: The influence factors entered the regression equation were ways of detoxification for drug abusers, marital status, mode of drug abuse, frequency of detoxification, maternal educational level, maternal occupation, family atmosphere and help and encourage from their relatives and friends, with their hazard ratios of 0.673, 1.315, 1.319, 0.867, 1.120, 1.318, 0.724 and 1.150, respectively. CONCLUSION: Combined statistical analysis of quality of life and survival time was a better method for the evaluation of the effectiveness of detoxification and its influence factors, which depended mainly on their mother's qualification, family atmosphere, mode and length of drug abuse, etc.

Humans↗
PubMed1999

[A clinicopathological study of pancreatoblastoma].

OBJECTIVE: To investigate the clinicopathological, immunohistochemical features and the histogenesis of pancreatoblastoma (PB) in 14 pediatric cases, including 9 male and 5 female patients, their age ranged from 1.5 to 8 years with a mean of 4.4 years. METHODS: Routine pathological, histochemical, and immunohistochemical methods were utilized to analyse the PB specimens. RESULTS: Tumors of 6 patients had metastasized to the liver, spleen and lymph nodes, 8 patients were alive 10 months to 105 months after diagnosis. Histologically, the tumors were composed of dense epithelial elements separated by fibro-stromal tissue, resulting in a nesting or organoid pattern. Immunohistochemically and histochemically, the tumors exhibited acinar, endocrine and ductal differentiation. The positive rates for were: CK 100%, EMA 86%, NSE 79%, CEA 71%, SYN 64%, CgA 43%, alpha1-AT 57%, PAS 100% and mucicarmine 57%. CONCLUSIONS: PB is the most common malignant pancreatic neoplasm of childhood. PB arises from primitive pluripotential cells capable of differentiating into 3 pancreatic cell types: acinar, endocrine and ductal. The prognosis is better than pancreatic carcinoma in adults.

Carcinoembryonic Antigen↗
PubMed1999

[Preventing and treating effect of total oil of Microula sikkimensis (Hemsl.) on experimental hyperlipemia in rats].

OBJECTIVE: To study the effect of the total oil of Microula sikkimensis on an experimental hyperlipemia model of rat. METHOD: Using feeding method to set up the experimental hyperlipemia model and determining the biochemical criteria. RESULT: The total oil of Microula sikkimensis significantly decreased the contents of cholesterol, triglyceride and MDA in blood serum, and increased the rate between total cholesterol and high density lipoprotein. CONCLUSION: The study provides an important basis for exploiting and utilizing the total oil of Microula sikkimensis.

Animals↗
PubMed1999

[Analysis of C5 fractions and carbonyl compounds by capillary gas chromatography].

We used two capillary columns with different characters--SE-54 (30 m x 0.25 mm) and Squalane (92 m x 0.24 mm) to analyse C5 fractions and carbonyl compounds in the mixtures of complex chemical composition. The physical properties of some components are very similar, especially for 1,3-cyclopentadiene and cis-1,3-pentadiene. 1,3-Cyclopentadiene in the feed will directly affect the activity of catalyst. In the past we only used SE-54 column to separate 1,3-cyclopentadiene and cis-1,3-pentadiene, but the results were unsatisfactory. Later we found that squalane column could separate them fairly good, but it could not yet separate dicyclopentadiene from 1,3-cyclopentadiene and carbonyl compounds in C5 fractions very well. In this work SE-54 capillary column and squalane capillary column were used together to analyse C5 fractions, and SE-54 column was used to analyse carbonyl compounds. The results are satisfactory.

Carbon↗
PubMed1999

[Laryngotracheobronchial involvement in relapsing polychondritis].

OBJECTIVE: To investigate the clinical cause, treatments and prognosis of laryngotracheobronchial involvement in relapsing polychondritis (RP). METHODS: Thirteen patients of RP with laryngotracheobronchial involvement were review retrospectively. RESULTS: From 1983 to 1998, 20 patients with RP were managed in this hospital. Of them, 13 (65%) involving the laryngotracheobronchial region were observed. Respiratory tract lesions might be diffuse or localized and occurred in larynx (46%), trachea (69%) and bronchus (69%). Corticosteroids, immunosuppressive agents and dapsone were used in these patients. Tracheotomy was performed in 4 patients with airway obstruction. Montgomery T-tube or self-expanding metal stent were used in 2 patients with tracheal collapse. Of 13 case, 2 died from respiratory complications. The 5-years survival rate was 78.6%. CONCLUSION: Respiratory tract involvement is the most severe manifestation of the relapsing polychondritis and the main cause of death despite aggressive medical therapy or tracheotomy.

Adrenal Cortex Hormones↗
PubMed1999

[Studies on the infrared spectra of mico2-OR or micro2-SR-bridged double-cubane-like clusters containing MoFe3S4 cores].

The IR spectra of micro2-OR or micro2-SR- bridged double-cubane-like clusters containing MoFe3S4 cores (Et4N)3 [Mo2Fe6S8Cl6 (OMe)3] (1), (Et4N) [Mo2Fe6S8 (SPh)6 (OMe)3 (2), (Bu4N)3 Mo2Fe6S (SPh)6 (OMe)3] (3), (Et4N)3 [Mo2Fe6S8 (SBu(t))6(OMe)3](4), (Et4N)3[Mo2Fe6S8 (SPh)9](5) have been investigated. Their characteristic frequencies (v)Mo-micro2O(R), (v)Mo-micro2S(R), (v)Mo-S, (v)Fe-S*, (v)Fe-SR, (v)Fe-Cl were assigned by comparing their vibrational frequencies with their structural parameters and by comparing the IR spectra of them with that of the linear clusters. The electrophilic induced effects in different way and the relationship between deltav (i.e. (v)Mo-S* (v)Fe-S*) and Fe --> Mo charge transfer have been discussed. Finaly, the sameness and difference between the double-cubane-like and linear Mo-Fe-S clusters were demonstrated.

English Abstract↗
PubMed1998

Spatial and temporal expression of AP-1 responsive rod photoreceptor genes and bZIP transcription factors during development of the rat retina.

PURPOSE: The promoter region of the rod-specific beta subunit of cGMP PDE (beta-PDE) and opsin genes contains highly conserved cis-acting elements, which include an AP-1 and/or Nrl response element (NRE: An extended AP-1 like sequence). Transactivation of AP-1 or NRE appears necessary to drive expression of these rod-specific genes during adulthood, however, their role during development is relatively unknown. Therefore, we determined the spatial and temporal relationships between rod morphological and functional development, rod-specific gene expression, and expression of the bZIP transcription factors c-fos, junD and Nrl. METHODS: Retinas from 0-45 day old (PN0-45) dark- and light-adapted Long-Evans rats were used. Morphological development was monitored by light and electron microscopy. Whole retinal trypsin-activated cGMP-PDE activity and rhodopsin content were measured biochemically. The expression of opsin, beta-PDE, c-fos, junD and Nrl mRNAs were determined by Northern blot analysis. The cellular localization of Nrl was examined with in situ hybridization. RESULTS: The mRNAs for opsin, beta-PDE and c-fos were observed at PN0-2, while cGMP-PDE activity and rhodopsin were detected first at PN5: coincident with rod outer segment development. The developmental pattern of cGMP-PDE activity and rhodopsin accumulation paralleled the expression of beta-PDE and opsin mRNA and all reached their maximal levels by PN45. Nrl expression, for all three transcripts found in the rat retina, was low on PN2 and reached its maximal level at PN14. The c-fos and Nrl expression preceded beta-PDE and opsin mRNA expression by 1-2 days. Nrl expression was detected first in the distal post-mitotic retina at PN5 and then in all nuclear layers during retinal development. Maximal expression shifted from the ganglion cells to the outer nuclear layer as the neural retina matured. In contrast, junD expression was highest at PN0 and declined to a stable level by PN10. CONCLUSIONS: Colocalization of Nrl and c-Fos suggests that expression of rod-specific genes, which utilize AP-1 or NRE sites in their promoter, could be regulated through the formation of Nrl-Fos dimers. We hypothesize that Nrl and c-Fos play a fundamental role in the initiation and regulation of the rod-specific gene expression in developing and adult rod photoreceptors.

3',5'-Cyclic-GMP Phosphodiesterases↗