Search PubMed⌕ Search

Biomedical subjects

L Haas

Publications and source records attributed to L Haas.

At least 73 records · Page 4Linked to original sources
PubMed1988

Detection of Aleutian disease virus DNA in tissues of naturally infected mink.

Organs of naturally infected mink were examined for the presence of Aleutian disease virus (ADV) DNA by in situ hybridization. Spleen, lymph nodes, thymus, bone marrow, kidney, liver, lung and small intestine were found to be positive for ADV to differing extents. Infected lymphoid organs showed a focal distribution of positive cells. Southern blot analysis of DNA extracted from infected organs revealed replicative forms of viral DNA in spleen and bone marrow samples only. These findings are consistent with a lymphotropism of ADV in vivo. Compared to the situation after experimental infection of mink these results indicate additional sites of virus replication and/or persistence of the naturally occurring disease.

Aleutian Mink Disease↗
PubMed1987

[Reuse of angiography catheters. II. Effect of multiple sterilizations on the properties of the catheter material--physicochemical studies].

The reuse of sterile medical devices designated for single use is a controversial practice, known to be performed in many countries. As far as catheters for angiography are concerned, various methods for cleaning and sterilisation are in use. However, interactions of detergents and ethylene oxide used in reprocessing with polyethylene materials of the catheters have not been investigated systematically. This paper presents a physico-chemical characterisation of common angiographic catheters. The interaction of polyethylene and ethylene oxide is examined.

Angiography↗
PubMed1987

[Recycling of angiography catheters. III. Effect of multiple sterilizations on the characteristics of catheter material. Experimental studies on mechanical behavior].

Reprocessing of angiographic catheters is becoming common practice. Physico-chemical and mechanical integrity of polyethylene, however, is conditional for any method of reprocessing in use. In this paper data are presented concerning mechanical stability of polyethylene catheters when repeatedly exposed to ethylene oxide, leading to the conclusion that reuse of catheters seems to be possible without loss of mechanical safety.

Angiography↗
PubMed1986

Replication of Aleutian disease virus in mink lymphocytes infected in vitro.

The kinetics of Aleutian disease virus (ADV) replication in mink lymphocytes was followed by the analysis of virus-specific antigens, infectious virus, and viral DNA. Stimulation with pokeweed mitogen (PWM; 20 micrograms/ml) increased the synthesis of ADV DNA in cultivated cells of the B-cell fraction. Maximum virus titers [10(6.5) fluorescence-forming units (FFU)/ml] were achieved after incubating infected cells for 60 h at 32 degrees. At this time, 10.4% of the cells in the B-cell-enriched fraction contained ADV-specific antigens and there was an average of 125 ADV genome equivalents per antigen-positive cell. ADV replication also was detected in T-cell-enriched fractions (with up to 10(5.3) FFU/ml, 5.3% of cells were antigen-positive, with 20 ADV genome equivalents per antigen-positive cell), but was 10 times lower compared with ADV replication in the B-cell fraction.

Aleutian Mink Disease Virus↗