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Biomedical subjects

L H Wu

Publications and source records attributed to L H Wu.

86 records · Page 5Linked to original sources
PubMed1982

Bronchogenic carcinoma: a study of patients treated at Capital Hospital, Peking, China.

From 1961 to 1972, 123 patients with lung cancer underwent operations at Capital Hospital, Peking, China. Ninety-six patients had resectable lesions and 27 did not, a resectability rate of 78%. Four patients (4.2%) died immediately postoperatively. Complications occurred in 8 (8.3%) patients. Five-year survival in this group of 92 survivors was 26 (28.3%). Ten-year survival among 49 patients was 10 (20.4%). Among the 92 five-year survivors, 41.2% had squamous cell carcinoma, 25% had adenocarcinoma, and 16% had undifferentiated carcinoma. Patients with squamous cell carcinoma had a much longer survival than the others. Six factors appear to influence survival after resection: cell type, presence of lymph node metastases, presence of tumor emboli in blood vessels, sex, age, and location. Men about 50 years of age, with a peripherally located squamous cell tumor and with no tumor emboli or lymph node metastases, have a good chance of surviving a pulmonary resection for ten years.

Adenocarcinoma↗
PubMed1979

Manual fluorometry of phenylalanine from blood specimens collected on filter paper: a modified procedure.

In this manual fluorometric method, blood samples are used that have been impregnated on the filter paper, a convenient collection technique that is widely used to screen newborns for phenylketonuria. The modified procedure, based on the method of McCaman and Robins [J. Lab Clin. Med. 59, 885 (1962)], includes elution of phenylalanine from specimens on filter paper, removal of proteins by precipitation with trichloroacetic acid at 0 degrees C, and then reaction with ninhydrin-peptide reagent for color development. The standard curve is linear to at least 200 mg/L and the CV is 6.3% for a phenylalanine concentration of 27 mg/L. The modified procedure is suitable both for early screening for phenylketonuria and for monitoring blood phenylalanine of phenylketonurics during dietary therapy.

Blood Specimen Collection↗
PubMed1978

Serum creatine phosphokinase and pyruvate kinase in neuromuscular disorders and Duchenne dystrophy carriers.

Serum levels of creatine phosphokinase (CPK) and pyruvic kinase (PK) were determined in 42 controls, 57 patients with various neuromuscular disorders, and 23 female relatives of Duchenne dystrophy patients. CPK and PK enzyme activities were increased in comparable numbers of muscle disease patients and female carriers; there was no advantage in the combined use of the two tests. We did not confirm earlier reports suggesting that determination of PK is more valuable than CPK in carrier detection.

Adolescent↗
PubMedDate not supplied

Survival of faecal coliforms and hygiene risks in soils treated with municipal sewage sludges.

An incubation experiment was conducted to monitor effect of sewage sludge application on changes in numbers of faecal coliforms in soils over time after sludge application and evaluate the hygiene risks. Soil faecal coliform counts were made after 1, 7, 14, 28, 56 and 84 days of incubation. The faecal coliform counts in the sludge-treated soils decreased substantially with time and were similar to those in the untreated controls after incubation for 56 days. Land application of air-dried sludges increased the hygiene risks due to the re-growth of faecal coliforms, and the counts of faecal coliforms in soil treated with air dried sludge from Suzhou (91% DM) were 50 times higher than in soils with fresh dewatered sludge from Suzhou (15% DM) after 7 days of incubation. The main factors affecting the changes in faecal coliform counts were sludge type and incubation time. Sludge type determined the faecal coliform counts and the ability of the faecal coliforms to re-establish, and indigenous microorganisms competed with the faecal coliforms for nutrients during the incubation process.

Animals↗
PubMedDate not supplied

Degradation of benzo[a]pyrene in soil with arbuscular mycorrhizal alfalfa.

Mycorrhizal and non-mycorrhizal alfalfa (Medicago sativa) was grown in pots containing soil artificially contaminated with various levels of benzo[a]pyrene (B[a]P) (0, 1, 10 and 100 mg kg(-1)). Soil and plants were sampled after 30, 40, 50, 60 and 90 days and compared with unlanted pots. The percentage of mycorrhizal root length colonized by Glomus caledoniun was not significantly affected by the addition of B[a]P up to 10 mg kg(-1) but was significantly lower at 100 mg kg(-1)B[a]P compared with low concentrations (p < 0.05). There was no difference in soil polyphenol oxidase and dehydrogenase activity among the controls and applications of 1 and 10 mg kg(-1) of B[a]P. However, enzyme activities were significantly higher at 100 mg kg(-1) B[a]P compared with the other three treatments, and there was no mycorrhizal effect. Over a period of 90 days the concentration of B[a]P in soil in which alfalfa was grown was significantly lower than in unplanted soil (p < 0.05). Degradation rates of B[a]P added at 1, 10 and 100 mg kg(-1) without G. caledonium were 76, 78 and 53%, and with mycorrhizal inoculation were 86, 87 and 57%. The degradation rate in unplanted soil was significantly lower than in planted soil, and was significantly higher in medium- and low-B[a]P treatments than in the high B[a]P concentration tested. There is a possibility of enhancement phytoremediation of PAHs in rhizosphere soil with arbuscular mycorrhizal fungi.

Benzo(a)pyrene↗
PubMedDate not supplied

Nutrients can enhance phytoremediation of copper-polluted soil by Indian mustard.

An orthogonally designed experiment was conducted to study the single and combined effects of N, P and K on phytoremediation of Cu-polluted soil by Indian mustard. Addition of fertilizer N and P significantly increased Indian mustard shoot yield. Two high treatments combined with N resulted in the highest yields, followed by low-P combined with N. In contrast, high P with no N gave no yield increase and K had no effect on yield of Indian mustard. Nitrogen and P increased the amount of chlorophyll in the leaves, indicating that the yield increases were due to enhanced photosynthesis. Nitrogen application had no effect on plant Cu concentrations but addition of P slightly decreased plant Cu concentrations, likely a dilution effect resulting from the increase in yield. Among the treatments, N and P applied at 100 and 200 mg kg(-1) respectively with no K application resulted in the highest Cu uptake. Thus, a combination of low N and high P produced a yield increase in Indian mustard that was more than adequate to compensate for a slight decrease in Cu concentration, resulting in the highest Cu removal from the contaminated soil.

Biodegradation, Environmental↗