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Biomedical subjects

L H Ma

Publications and source records attributed to L H Ma.

10 recordsLinked to original sources
PubMed2006

Association between polymorphisms of vitamin D receptor gene ApaI, BsmI and TaqI and muscular strength in young Chinese women.

UNLABELLED: The association between the polymorphisms of vitamin D receptor gene ApaI, BsmI and TaqI and isokinetic muscular strength was examined in 109 healthy, young Chinese women. METHODS: Genomic DNA was extracted from mouthwash samples and vitamin D receptor genotypes were determined with the established methods. The peak torque of the dominant limb in concentric and eccentric knee extensors and flexors and elbow extensors and flexors was measured using Cybex-Norm-770 isokinetic dynamometer at slow and fast velocities. The distribution frequency of vitamin D receptor gene alleles was analyzed by chi-square test. The difference in muscular strength between VDR genotype groups was analyzed using one-way ANOVA. The ApaI, BsmI and TaqI alleles and genotype frequencies appeared to be in a Hardy-Weinberg equilibrium. Subjects with the vitamin D receptor ApaI null (A allele) mutation (AA) exhibited significantly lower knee and elbow concentric or eccentric peak torque than those with aa homozygous or Aa heterozygous. The BB & Bb group showed significantly higher peak torque in concentric knee flexors than bb group. No association was found between vitamin D receptor gene TaqI and muscular strength. The data indicated that vitamin D receptor gene ApaI and BsmI polymorphisms rather than TaqI polymorphism might be associated with muscular strength.

Adult↗
PubMed2002

Culture-derived Babesia orientalis exoantigens used as a vaccine against buffalo babesiosis.

Buffalo babesiosis represents a major problem for the livestock industry in China. The pathogen of this disease was isolated and identified as Babesia orientalis and subsequently propagated in vitro, using the microaerophilus stationary phase culture system. The aim of this study was to determine the efficacy of exoantigens derived from B. orientalis cultures to induce a protective immunity against challenge exposure to virulent organisms in laboratory and field. The results showed that exoantigens of B. orientalis could induce a high degree of protection against challenge with a virulent B. orientalis strain. The animals in the vaccinated group exhibited a slight decrease in haemoglobin levels and blood cell counts, whereas animals in the control group showed typical clinical symptoms and died between days 11 and 16 after challenge. In another approach, 82 buffaloes kept in B. orientalis-endemic areas were injected with exoantigens plus Freund's adjuvant in two doses at an interval of 2 weeks, one month before the endemic period. An additional 86 buffaloes served as unvaccinated controls. During the endemic period, the clinical signs of all buffaloes were monitored and the antibody response was investigated, using the latex agglutination test. During the endemic period, none of the vaccinated buffaloes showed clinical signs or died, whereas five control buffaloes showed clinical signs and two of them died. The mean antibody titre of vaccinated animals was higher than that of the control animals. These results showed that the culture-derived exoantigens of B. orientalis could be used as a vaccine to prevent buffalo babesiosis.

Animals↗
PubMed2000

[Analysis on genetic polymorphism of mtDNA in endurance athletes and sedentary subjects].

AIM: To analysis the sequence variation of the origin region (D-loop) of mitochondrial DNA (mtDNA) in elite endurance athletes and sedentary subjects in order to find the genetic markers related with performance. METHODS: Using a new established PCR method by virtue of tracing sample analysis, the restricted fragment length polymorphism (RFLP) of D-loop in mtDNA was assessed in 76 elite Chinese endurance athletes (EEA), 33 endurance athletes with average-level(GEA) and 20 sedentary control(sc). RESULTS: There is a significant difference in distribution of the polymorphism in mtDNA D-loop between the EEA and SC(chi 2 = 33.3, P < 0.01). CONCLUSION: The sequence variation of D-loop region of mtDNA in the elite endurance athletes may contribute to the individual difference in aerobic performance and trainability.

Adult↗
PubMed1992

Protective effect of zinc on liver injury induced by D-galactosamine in rats.

The protective effects of zinc on liver injury induced by D-galactosamine (GalN) were investigated in rats in vivo and in vitro. Zinc supplementation (50 mg/kg/d) for 5 d of rats treated with GalN (1.5 g/kg, ip) could reduce their mortality rate, restore liver pathomorphological changes, maintain zinc content, inhibit the lipid peroxidation, hasten the protein synthesis, and improve liver function. In vitro, zinc supplement could abate the death of GalN-intoxicated hepatocytes, decrease malonaldehyde (MDA) content, and maintain reduced glutathione (GSH). It is concluded that zinc has protective effects on GalN-induced liver damage. Its effects may be owing to inhibition of lipid peroxidation and hastening of protein syntheses.

Animals↗
PubMed1991

Effect of tumor necrosis factor-alpha on the proliferation of leukemic cells from children with B-cell precursor-acute lymphoblastic leukemia (BCP-ALL): studies of primary leukemic cells and BCP-ALL cell lines.

The effect of recombinant tumor necrosis factor-alpha (rTNF-alpha) on the primary leukemic blasts and leukemic cell lines derived from children with B-cell precursor acute lymphoblastic leukemia (BCP-ALL) was studied. The proliferation of leukemic cells from the bone marrow of 11 of 13 patients (seven at diagnosis, four in relapse) and from the 697 (BCP-ALL) cell line was significantly inhibited by rTNF-alpha at the lowest dose tested (0.1 ng/mL), as measured by 3H-TdR uptake. The degree of inhibition was variable, ranging from 17% to 78%. Furthermore, a dose-dependent inhibitory effect was observed, with approximately 70% mean inhibition of DNA synthesis detected when cells from 12 of 13 patients were incubated with 100 ng/mL of rTNF-alpha for 3 days. In contrast, rTNF-alpha did not inhibit another BCP-ALL cell line (EU-1/ALL) established recently in our laboratory. Studies indicated that the TNF-alpha gene was expressed by the primary leukemic blasts of one TNF-resistant case in his third relapse and by EU-1 cells. Also, TNF-alpha protein was detected by Western blot analysis and enzyme-linked immunoabsorbent assay in the supernatant of EU-1 cells; this is the first report of TNF production by a BCP-ALL cell lines. The production of TNF-alpha mRNA and protein was not detected in the 697 cell line and in the primary leukemic blasts from six patients (four at diagnosis, two in relapse) whose leukemic cells were inhibited by TNF. The partially purified TNF-alpha obtained from the EU-1 cell line also suppressed the proliferation of TNF-sensitive primary leukemic cells, and this inhibitory activity was abolished by an anti-TNF-alpha specific antibody. Our results demonstrate that TNF-alpha is an inhibitor of in vitro proliferation of BCP-ALL cells from most patients. The TNF-resistant leukemic cells from a few patients and the EU-1 cell line express TNF mRNA, suggesting that the induction of TNF gene expression is associated with the development of TNF resistance.

Blotting, Western↗
PubMed1989

Physical growth of Chinese children from birth to 6 years old in urban and rural areas in 1985.

During 1985 a survey of the physical growth of 175,290 Chinese children less than 7 years in age was undertaken in the urban and rural areas of ten provinces. The results of weight, height, and head circumference are similar to those generally found as regards the differences between boys and girls. The children in rural areas were lighter and shorter. From the age of 3-6 months onwards the weights and heights of all groups were less than international standards. Nutrition is probably the major factor in these differences in growth.

Body Height↗