Biomedical subjects
Publications and source records attributed to L Guilloux.
The authors discuss different in vitro tests that provide evidence for: involvement of atopy in clinical symptoms (eosinophilia, total IgE titre, histaminemia); the identification of the responsible allergen (specific IgE, basophil degranulation, and release of histamine); following the course of hyposensitization treatment (specific IgG).
In the present work a study has been made of the prevalence of allergy to two storage mites, Tyrophagus putrescentiae and Lepidoglyphus destructor in a population of 105 young adults in the east of France. Combination of skin tests and specific IgE measurements shows that 43.10% of the subjects were sensitive to Tyrophagus and 44.95% to Lepidoglyphus. Interpretation of skin tests remains uncertain, particularly concerning the source of the allergen used and it is most often also necessary to measure the specific serum IgE. As in many studies, most sensitization was found amongst farmers. In contrast, no difference was seen between rural and city dwellers, subjects who were in contact with small animals and those in contact with food for livestock if they were not farmers, not between declared asthmatics who lived in a humid place and those who lived in an environment that was drier. Certainly these mites appeared in the present study to be much more ubiquitous than supposed previously and the fact that they seemed to be carriers of allergens and antigens that were different from those of Dermatophagoides raises the question as to whether the need for hyposensitization treatment for them has been under-estimated.
The goal of this work was the study of the prevalence of allergy to two mites, Tyrophagus putrescentiae and Lepidoglyphus destructor in a population of 105 young asthmatic men from East of France. The association of skin tests and specific IgE shows that 43.10% are allergic to Tyrophagus and 44.95% to Lepidoglyphus destructor. The significance of skin tests is doubtful and depends on the laboratories which provide the allergens. Therefore, specific IgE has to be performed. As in other studies it has been observed that farmers are very often sensitized. But, nose difference has been noticed between people living in town or in country, between those having contacts with pets or not, feeding the cattle or not (except for the farmers). In the same way, no difference has been noticed between the asthmatics living in a wet country and/or wet house and those living in a dry area.
PHA polypeptides were separated by SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose filters which were then cut into identical discs and used to perform blastogenic stimulation on cultured human peripheral blood lymphocytes. This method offers the following advantages: the high resolution of SDS-polyacrylamide gel electrophoresis, the sensitive immunochemical identification of the polypeptides involved in the mitogenic responses of lymphocytes, the determination in the same experiment of the molecular weight, antigenicity and biological activities of the proteins.
Rapid, specific and reproducible radioimmunoprecipitation assay (RIP) for measuring IgG antibodies against Yellow Jacket Venom (YJV) has been developed. The YJV-I125 is added to serum sample and the immune complexes are precipitated by a second antibody with polyethylene glycol. IgG antibodies were measured by RIP in 24 patients receiving Yellow Jacket Venom immunotherapy. RIP assays were compared for 159 sera to a solid phase procedure (RAST IgG).
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Phadiatop is a new in vitro screening test for respiratory allergy. This test, based on the RAST procedure, detects in serum, the presence of specific IgE to a mixture of common inhalent allergens. Among 70 patients (26 children and 44 adults) consulting for respiratory syndrome, Phadiatop was positive in 31 cases. There were a good correlation between this new test and skin tests (59% for adults and 92% for children), total IgE (70% for adults and 65% for children) and RAST (93% for adults and 96% for children). Phadiatop, with a specificity of 100%, a sensitivity of 82% (76% for adults and 92% for children) and an efficiency of 90% (86% for adults and 96% for children), is a more accurate test than total IgE and could be an excellent in vitro screening test for respiratory allergy.
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