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Biomedical subjects

L Glasser

Publications and source records attributed to L Glasser.

59 records · Page 4Linked to original sources
PubMedDate not supplied

The effect of platelets and red cells on granulocytes stored at 22 degrees C.

Granulocyte concentrates contain varying numbers of platelets and red cells depending upon the method of collection. Either platelet or red cell concentrations may be as high as 2.0 X 10(12) per I. Studies were done on unwashed and washed granulocyte concentrates and on pure granulocyte suspensions with known numbers of platelets or red cells added. These suspensions or concentrates were stored for 72 hours at 22 degrees C. In both experiments, the following were measured: leukocyte and absolute granulocyte counts, dye exclusion, chemotaxis, plasma glucose, plasma pH, and osmolality. Red cell contamination did not adversely effect granulocyte storage. Platelets, however, did contribute to the functional deterioration of stored granulocytes. In the presence of high concentrations of platelets, both granulocyte dye exclusion and chemotaxis were adversely affected at 48 hours of storage. In another experiment, fresh autologous granulocytes incubated for 18 hours in hydroxyethyl starch-citrated-plasma obtained from stored granulocyte concentrates showed a progressive decrease in chemotaxis related to the age of the stored plasma. Glucose supplementation of the spent plasma maintained chemotactic activity. Contamination of granulocyte concentrates with other cells and the availability of glucose to granulocytes are variables affecting the short-term liquid storage of granulocytes at 22 degrees C.

Blood Glucose↗
PubMedDate not supplied

Granulocyte concentrates. Glucose concentrations and glucose utilization during storage at 22 degrees C.

Neutrophil metabolism is dependent on the available stores of either exogenous or endogenous glucose. We studied 38 granulocyte concentrates collected by four different centrifugal cytapheresis systems: Haemonetics model 30, IBM 2997, Fenwal CS-3000, and Fenwal Celltrifuge II. Extracellular glucose concentrations were measured immediately following collection and after 24 hours of storage. The Fenwal CS-3000 produced the highest concentrations of platelets and leukocytes, and the units had the lowest initial glucose levels and pH. After 24 hours of storage, there was a mean glucose of 1.7 mg per dl in the Fenwal CS-3000 granulocyte concentrates and 9 of 12 units had no detectable glucose. In contrast, concentrates collected from other systems had sufficient glucose available to support neutrophil metabolism for a longer period.

Blood Glucose↗
PubMedDate not supplied

Neutrophil collection using modified fluid gelatin. Effect on in vitro and in vivo neutrophil function.

Modified fluid gelatin (MFG) is a possible alternative to hydroxyethyl starch (HES) for use as a red cell sedimenting agent in neutrophil collection procedures. Since the efficacy of neutrophil transfusion therapy depends upon the integrity of the infused cells, we examined the in vitro and in vivo function of neutrophils collecting using MFG. Neutrophils were collected from 17 normal subjects by continuous flow centrifugation using MFG in place of HES. In vitro measurement of neutrophil function included dye exclusion, phagocytosis, candidacidal activity, staphylococcal killing, chemotaxis, and random migration. For in vivo studies, neutrophils were labeled with 3H-diisopropylfluorophosphate, reinfused into the donor, and blood kinetics and skin chamber accumulation of the labeled cells were measured. In vivo results were compared with results from previous studies using neutrophils collected with HES or by phlebotomy. MFG neutrophils were normal by all in vitro functional criteria and localized normally at an in vivo inflammatory site. Intravascular survival (T 1/2 = 3.3 +/- 0.9 hours) was significantly shorter than normal (T 1/2 = 7.3 +/- 2.3 hours) but was identical to that of cells collected using HES. Thus, on the basis of neutrophil functional capabilities, MFG appears to be an acceptable alternative to HES in collection of neutrophils for transfusion.

Cell Separation↗
PubMedDate not supplied

Modified fluid gelatin. An alternative macromolecular agent for centrifugal leukapheresis.

We studied a French modified fluid gelatin (MFG), substituting it for hydroxyethyl starch (HES) in leukapheresis procedures using three currently available blood cell separators, and observing its effects on the function of platelets and granulocytes. As a cell-collecting agent, we found MFG to be as effective as HES with intermittent flow centrifugation (Haemonetics), and slightly less so with one continuous flow device (IBM 2997). MFG was clearly less effective than HES with the Fenwal CS-3000 continuous flow separator, although we have reason to believe it would be possible to improve efficiency with this machine by changing the operating variables. Tests of platelet and granulocyte function showed negligible alteration with either agent and no difference between them. MFG disappears much more rapidly from the circulation than HES (after a single injection, it is undetectable by the third day). Reaction frequency with MFG was about the same as that of HES, with perhaps somewhat more frequent allergic manifestations.

Blood Platelets↗