Cytoplasmic crystalloids in the ovary of a woolly monkey.
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Animals↗
Biomedical subjects
L G Adams
Publications and source records attributed to L G Adams.
An experimental infection of Trypanosoma cruzi in striped skunks (Mephitis mephitis).
Four adult (3 male, 1 female) captive-raised, decented, striped skunks (Mephritis mephitis) were infected experimentaly with a field strain (Texas-Tulane) of Trypanosoma cruzi, originally isolated from a naturally-infected dog. Two skunks were injected intravenously with approximately 4.5 x 10(6) viable T. cruzi trypomastigotes. Two skunks were inoculated per os and per conjunctivum with 10 ml of phosphate buffered saline containing macerated, T. cruzi-infected triatomine intestines and intestinal contents. The skunks had minimal clinical manifestations with no mortalities occurring during 46 days post-exposure. Sera from all skunks were positive at 24 days post-inoculation (PI) by the direct and latex agglutination tests. Blood cultures from the 4 skunksd were positive for T. cruzi at day 24 PI and 3 were positive at day 46 PI. All skunks had mild to moderately severe chronic granulomatous myocarditis of the atria and ventricles. Typical T. cruzi amastigotes were present within myocardial fibers in 3 of 4 skunks.
Animals↗
A study of the toxicity of imidocarb dipropionate in cattle.
The toxic effects of imidocarb dipropionate (3,3'-bis[2-imidazolin-2-yl] carbanilide dipropionate) were studied in calves injected twice intramuscularly with 0, 5, 10 or 20 mg/kg dosages. Transient, dosage dependent signs of toxicosis consisted of excessive salivation, serous nasal discharge, diarrhoea and dyspnoea. Elevations in blood urea nitrogen concentrations and serum glutamic oxalacetic transaminase activities were related to dosage and markedly increased at the high dosage. Renal hyperaemia, hepatomegaly, pulmonary congestion and oedema, hydrothorax, hydroperitoneum, hydropericardium and mortality occurred at the 20 mg/kg dosage. Microscopic lesions observed at the high dosage included acute severe renal tubular necrosis and focal hepatocellular necrosis. Injection site reactions varied from microscopic areas of necrotising myositis at the 5 mg/kg dosage to focal grossly visible areas of necrosis, encapsulated by granulation tissue and surrounded by fascial oedema at the 20 mg/kg dosage.
Animals↗
Serum complement and blood platelet adhesiveness in acute canine ehrlichiosis.
Three dogs inoculated with Ehrlichia canis served as infected controls (group I), 3 dogs treated with cobra venom factor (CVF) after inoculation with E canis were the principals (group II), 3 noninfected dogs were the noninfected controls (group III), and 1 noninfected dog given CVF was the noninfected, decomplemented control (group IV). Thrombocytopenia occurred in all E. canis-inoculated dogs (groups I and II) within 3 weeks after they were inoculated. However, the severity of the thrombocytopenia was moderated in the group II dogs (decomplemented E canis-inoculated). Hypocomplementemia occurred in the CVF-treated dogs (groups II and IV) immediately and persisted for up to 5 days after administration of CVF. Hypocomplementamia also occurred in all E canis-infected dogs (groups I and II) during the period of maximal thrombocytopenia. The mean platelet retention was significantly decreased in all E canis-inoculated dogs and in the CVF-control dog (groups I, II, and IV). There was no correlation between platelet retention and the number of platelets in group III (nontreated, noninoculated) controls. The correlation between the decrease in platelet retention and the number of platelets was significant in the complement-intact infected dogs, yet was not significant in the decomplemented infected dogs. Data indicated that complement consumption occurred in acute canine ehrlichiosis and coincided, to a degree, with the development of thrombocytopenia. Decomplementation of inoculated dogs moderated the severity of the thrombocytopenia. Platelet function is also altered in acute stages of the disease.
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Brucella abortus in coyotes. I. A serologic and bacteriologic survey in eastern Texas.
Prevalence of Brucella abortus serum antibodies in coyotes from east central Texas was determined by the buffered Brucella antigen (card test), rivanol, standard agglutination tube, and cold complement fixation tube tests. Eighteen percent (9 of 51) of the coyotes were positive serologically. B. abortus biotype 1 was isolated from various tissues from 7 of 43 coyotes by bacteriologic culture. Congenital transmission was found.
Animals↗
Effects of cyclophosphamide on the lymphoid tissues and humoral and cellular immune responsiveness of young calves.
Cyclophosphamide (CY) was given IV to 5-month-old calves (ten doses; each dose of 5.0 mg/kg, 2-day intervals between doses). The effects of CY on circulating leukocytes, lymphoid tissues, and the humoral and cellular immune responses were assessed. The numbers of total leukocytes, lymphocytes, and neutrophils and platelets decreased significantly. The lymphocyte population was depleted in the cortex of the thymus and B-dependent areas of the spleen and lymph nodes. Significant decreases occurred in the frequency of the peripheral blood lymphocytes-bearing surface immunoglobulin (Ig) and in serum IgM and IgG concentrations. Primary serum antibody responses to avian erythrocytes and Brucella abortus strain 19 antigens were diminished or delayed. The blastogenic responses of peripheral blood lymphocytes to phytohemagglutinin P, concanavalin A, pokeweed mitogen, and to purified protein derivative and B abortus antigens were enhanced as was the delayed hypersensitivity reaction to the tuberculin skin test. While a diminished humoral immune response was associated with CY treatment, the cell-mediated response was potentiated. The effect of CY was transitory with most variables returning to near base line within 24 days after CY was ceased.
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Methods to improve the health of cattle in the tropics: immunisation and chemoprophylaxis against haemoparasitic infections.
A study of methods to improve the health of native cattle in tropical areas of Colombia showed an advantage using immunisation techniques against haemoparasitic infections in comparison with other control methods. The control of anaplasmosis and babesiosis by immunisation of cattle with fully virulent Anaplasma marginale, Babesia argentina and B. bigemina is feasible in tropical cattle when the postimmunisation reaction is controlled by appropriate drug therapy. Chemoprophylaxis was found less effective in controlling haemoparasitic diseases; however, treated was found less effective in controlling haemoparasitic diseases; however, treated cattle surviving the acute stage of infection showed weight gains not significantly different from those of the immunised calves. Both methods were found to be advantageous with calves born and raised in an endemic area of anaplasmosis and babesiosis. Tick and gastrointestinal parasitic control without haemoparasitic control in calves had an advantage over no control system at all. These methods though were inferior to the immunisation and chemoprophylactic techniques.
Anaplasmosis↗
Methods to improve the health of cattle in the tropics: conclusions and economic appraisal.
The results of the immune responses of immunised and chemoprophylactically treated calves to tick-borne (Boophilus microplus) challenge indicate that the system of immunisation was effective in protecting cattle against Anaplasma marginale, Babesia argentina (bovis), and B. bigemina. However, chemoprophylaxis was effective only against Babesia spp. but not against A. marginale. Both methods showed a substantial advantage over no control system when using native cattle breeds in a zone endemic for bovine anaplasmosis and babesiosis. Based on the net economic gain per calf starting the experiment, sizeable differences were noted at 308 days between the calves in the immunised group, chemoprophylaxis group, tick and gastrointestinal parasite control group and the experiment control group.
Anaplasmosis↗
Lacrimal cyst (dacryops) in 2 dogs.
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Animals↗
Naturally occurring trypanosomiasis (Chagas' disease) in dogs.
Nine fatal cases of canine American trypanosomiasis (Chagas' disease) were encountered from November, 1972, through November, 1975. Of the 9 cases, 7 occurred in dogs from 5 central Texas counties, and all but one case was diagnosed during the months of September, October, or November. The source of infection was discovered in only one case--that being a doghouse heavily infested with Triatoma lectularius occulta. Each of 6 bugs collected had infective forms of the protozoan, Trypanosoma cruzi. Trypanosomiasis usually is not considered in the differential clinical diagnosis of cardiac dysfunction in man or other mammals in the United States. An antemortem diagnosis was made in only 1 of the 9 dogs, with diagnosis in the remaining dogs being made at necropsy. The predominant microscopic lesion in all dogs was necrotizing granulomatous myocarditis associated with the amastigotes of T cruzi.
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Comparisons of the complement-fixation and indirect fluorescent antibody reactions in the detection of bovine babesiasis.
Complement-fixation (CF) and indirect fluorescent antibody (IFA) antigens were prepared from Babesia bigemina isolates obtained in Texas. These serologic procedures were evaluated on 130 serum samples sequentially collected from 5 B bigemina-infected mature cattle, beginning on the day of exposure and continuing for 175 day thereafter. Both tests were effective in detecting specific antibodies for the first 84 days of infection, with 57 of 60 (95%) serums tested being positive on the CF test and 57 of 57 (100%) tests being positive to the IFA test. During the interval from 98 to 175 days, 24 of 60 (40%) of the serums tested were positive with the CF test, and 53 of 56 (95%) were positive with the IFA test. During the first 84 days, a similar linear regression occurred in both CF and IFA serum titers, but after 98 days the IFA regression flattened out, whereas the CF titers decreased below the sensitivity threshold in 60% of the serums tested.
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Experimentally induced anaplasmosis in neonatal isohemolytic anemia-recovered calves.
Anaplasma marginale infections were induced in 3 calves previously affected with neonatal immunohemolytic anemia (NIA). Similar infections were induced in 6 splenectomized and 7 intact calves. The response to infection by 3 calves (NIA recovered) closely resembled infections seen in splenectomized calves, being markedly more severe than similar infections in intact calves. Spleens from 3 (NIA recovered) calves after splenectomy were about one-tenth normal size. Marked recrudescing anaplasmal infections were not detected after splenectomy of the calves (NIA recovered), whereas marked recrudescing infections were observed after splenectomy of 2 intact calves having recovered from the primary infections.
Anaplasmosis↗
Ultrastructural renal lesions in goats given a lethal dose of imidocarb diproprionate.
Ultrastructural renal lesions were studied in 20 goats given one intramuscular injection of a lethal dose of imidocarb diproprionate (6.75 mg/kg). The lesions were limited to proximal convoluted tubular epithelial cells and at postinjection (PI) hour 3 included: plasma membrane disruption, smooth endoplasmic reticulum proliferation and dilatation, swelling and increased electron density of mitochondria, and cytoplasmic ground substance rarefaction. At PI hour 12, disruption of plasma membranes, with fragmentation of the microvilli, loss of junctional complexes, and cellular detachments from in tact basement membranes were observed in many tubular epithelial cells. Mitochondrial configurational alterations became more prominent at 6 and 12 hours, with the formation of large membrane-bounded structures containing fragmented cristae, clear vacuoles, and amorphous matrical material. The intramitochondrial foci of electron-dense amorphous flocculent material, which was first observed at 6 hours and increased in electron density and in number by 12 and 24 hours, were probably comprised of denatured, clumped matrical protein. The 2nd type of intramitochondrial and cytoplasmic electron-dense deposit was observed PI hour 24. The basement membranes remained intact in most proximal tubules to support regenerating epithelial cells on PI day 4 and thereafter.
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Influence of dexamethasone on the recrudescence of Anaplasma marginale in splenectomized calves.
Dexamethasone was administered at the dose rate of 0.2 mg/kg of body weight to 11 splenectomized Anaplasma-carrier calves (groups 1 and 3) on Monday, Wednesday, and Friday for 3 weeks. Observations were made on these calves and on 7 nontreated, comparable calves (group 2) to determine the influence of treatment on carrier infections. Dexamethasone treatment was associated in every instance with an exacerbation of the Anaplasma parasitemia and a decrease in packed red cell volume. The episode of acute anaplasmosis was of short duration, resembling the primary response, except that complement-fixation response did not increase accordingly. Serum protein electrophoresis of serums from 4 calves (group 3) undergoing the drug-induced response failed to show any significant change during the 3-week treatment period, but did show a significant increase in gamma-globulin immediately after treatment.
Anaplasmosis↗
Hepatic fatty cirrhosis in Texas white-tailed deer.
Hepatic fatty cirrhosis (HFC) has been known to occur in certain domestic livestock species since 1931. Early studies in Texas indicated that HFC was restricted to five western counties. Recently HFC was identified in white-tailed deer (Odocoileus virginianus texanus) in South Texas for the first time. Of the deer examined, 25% were affected. The etiology of HFC was not determined.
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Clinical, histologic, and histochemical study of imidocarb diproprionate toxicosis in goats.
The toxic effects of imidocarb diproprionate (3,3'-bis [2 imidazolin-2yl]-carbanilde diproprionate) were evaluated in adult goats given (intramuscular injection) a lethal dose (6.75 mg/kg). The immediate clinical signs of toxicosis were transient excessive salivation and diarrhea. Anorexia, dyspnea, recumbency, and death occurred between postinjection days (PID) 4 and 8, during which time 7 goats died and 4 moribund goats were euthanatized. There were marked increases in mean serum urea nitrogen concentration and significant increases in serum glutamic oxalacetic transminase activity and in the mean number of circulating neutrophils after PID 4. Renal hyperemia and enlargement were evident by PID1. Serosanguineous fluid in the trachea and major bronchi, pulmonary congestion and edema, hydrothorax, hydroperitoneum, and less frequently hydropericardium were observed on and after day 4. Microscopic renal tubular lesions rapidly progressed from pyknotic epithelial nuclei observed at 6 and 12 hours to acute tubular necrosis of epithelium of the proximal convoluted tubules on days 1 and 2. Pulmonary congestion and edema; hemorrhage into alveoli, bronchioles, and bronchi; and intracytoplasmic lipid vacuoles within the hepatocytes in the periacinar zones of the hepatic lobules were observed on or after day 4. Succinic dehydrogenase and adenosine triphosphatase activities decreased progressively in the epithelial cells of the proximal convoluted tubules. The decreases in cellular enzymatic activity occurred shortly after the appearance of microscopic lesions in the tubular epithelium.
Adenosine Triphosphatases↗
Evaluation of the indirect fluorescent antibody test for detecting Trypanosoma vivax in South American cattle.
The indirect fluorescent antibody test (IFAT) as used in Africa for detecting bovine trypanosomiasis was adapted for use in South America and evaluated. Antigen consisted of Trypanosoma vivax laden bovine blood fixed in a 60 : 40 : : acetone : methanol solution. The test detected initial titres of 1 : 50 and 1 : 100 at an average of 13.1 and 15.9 days post parasitaemia (PP). Maximum titres as high as 1 : 400 developed in eight calves at an average of 23.4 days PP. In another calf, 109 days PP were required. Efficacy in detecting sero-positive calves throughout the course of infection was 81.1 and 96.4 per cent at serum dilutions of 1 : 100 and 1 : 50 respectively. No false positive reactions occurred when sera from 36 haemoparasite-free calves were tested. Cross reactivity did not occur when sera from calves singularly infected with Trypanosoma theileri, T evansi, Anaplasma marginale, Babesia argentina, B bigemina and Eperythrozoon spp were similarly tested in the IFAT. No significant differences were found in IFAT results of surveys in which both conventional serum samples and sera eluted from dried blood samples on filter paper from the same calf were used.
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Viral particles associated with malignant catarrhal fever in deer.
Viral particles associated with malignant catarrhal fever in white-tailed deer (Odocoileus virginianus) and axis deer (Asix axis) are described. Morphologically, the virus resembled the togaviruses and was unlike the herpesvirus which causes wildebeest-associated malignant catarrhal fever (snotsiekte).
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